LOCUS       sequence-23334-e         510 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  modified pENTR vector for C-terminal STREP tag fusion with your gene
            of interest.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Dubin MJ, Bowler C, Benvenuto G
  TITLE     A modified Gateway cloning strategy for overexpressing tagged 
            proteins in plants.
  JOURNAL   Plant Methods. 2008 Jan 22. 4(1):3.
  PUBMED    18211686
REFERENCE   2  (bases 1 to 510)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods. 2008 Jan 22. 4(1):3."
COMMENT     Sequence Label: pE1c
FEATURES             Location/Qualifiers
     source          1..510
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    96..195
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(213..236)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     protein_bind    complement(303..402)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(421..444)
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     terminator      complement(452..479)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 cttttncgng ttttttgttc atgatgatat atttttatct tgtgcaatgt aacatcagag
       61 attttgagac acgggccaga gctgcagctg gatggcaaat aatgatttta ttttgactga
      121 tagtgacctg ttcgttgcaa caaattgata agcaatgctt tcttataatg ccaactttgt
      181 acaagaaagc tgggtctaga tatctcgagc tatttttcga actgcggatg gctccatccg
      241 ccgcttgcgg ccgcatgtcc gaaatctgta ccggatccag tcgactgaat tggttccttt
      301 aaagcctgct tttttgtaca aagttggcat tataaaaaag cattgcttat caatttgttg
      361 caacgaacag gtcactatca gtcaaaataa aatcattatt tggggcccga gcttaagtaa
      421 ctaactaaca ggaagagttt gtagaaacgc aaaaaggcca tccgtcagga tggccttctg
      481 cttagtttga tgcctggcag tttatggcgg
//
