LOCUS       sequence-23352-e         915 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Yu X, Zhan X, D'Costa J, Tanavde VM, Ye Z, Peng T, Malehorn MT, Yang
            X, Civin CI, Cheng L
  TITLE     Lentiviral vectors with two independent internal promoters transfer 
            high-level expression of multiple transgenes to human hematopoietic 
            stem-progenitor cells.
  JOURNAL   Mol Ther. 2003 Jun . 7(6):827-38.
  PUBMED    12788657
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther. 
            2003 Jun . 7(6):827-38."
COMMENT     Sequence Label: EF.CMV.RFP
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     LTR             254..487
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    559..680
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(596..615)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     650..669
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      720..855
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(876..895)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(877..895)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tcgagcgcac cgagggccgc caccacctgt tcctgtagcg gccgcgactc tagatcataa
       61 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
      121 tgaacctgaa acataaaatg aatgcaattg ttgttgttga cgggcccgcg gaattcgagc
      181 tcggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
      241 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct
      301 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
      361 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
      421 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
      481 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
      541 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat
      601 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
      661 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca
      721 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
      781 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
      841 gcttttttgg aggcctaggg gacgtaccca attcgcccta tagtgagtcg tattacgcgc
      901 gctcactggc cgtcg
//
