LOCUS       sequence-233804-        2339 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Plasmid donor for expression of proteins under the control of a 
            minimal HSP70 promoter in baculovirus/insect cell systems.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Monika Golas
  TITLE     Plasmids for expression of proteins in insect cells
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pGS-BacD-23004
FEATURES             Location/Qualifiers
     source          1..2339
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     198..219
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     primer_bind     complement(323..342)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    367..415
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     protein_bind    617..650
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      652..1008
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     CDS             1473..2264
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cgatactagt cgccggagta taaatagagg cgcttcgtct acggagcgac aattcaattc
       61 aaacaagcaa agtgaacacg tcgctaagcg aaagctaagc aaataaacaa gcgcagctga
      121 acaagctaaa caatctgcag taaagtgcaa gttaaagtga atcaattaaa agtaaccagc
      181 aaccaagtaa atcaactgca actactgaaa tctgccaaga agtaattatt gaatacaaga
      241 agacttgatc acccgggatc tcgagccatg gtgctagcag ctgatgcata gcatgcggta
      301 ccgggagatg ggggaggcta actgaaacac ggaaggagac aataccggaa ggaacccgcg
      361 ctatgacggc aataaaaaga cagaataaaa cgcacgggtg ttgggtcgtt tgttcataaa
      421 cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattgggac
      481 caatacgccc gcgtttcttc cttttcccca ccccaacccc caagttcggg tgaaggccca
      541 gggctcgcag ccaacgtcgg ggcggcaagc cctgccatag ccactacggg tacgtttaaa
      601 cccatgtgcc tggcagataa cttcgtataa tgtatgctat acgaagttat ggtacgtact
      661 aagctctcat gtttcacgta ctaagctctc atgtttaacg tactaagctc tcatgtttaa
      721 cgaactaaac cctcatggct aacgtactaa gctctcatgg ctaacgtact aagctctcat
      781 gtttcacgta ctaagctctc atgtttgaac aataaaatta atataaatca gcaacttaaa
      841 tagcctctaa ggttttaagt tttataagaa aaaaaagaat atataaggct tttaaagctt
      901 ttaaggttta acggttgtgg acaacaagcc agggatgtaa cgcactgaga agcccttaga
      961 gcctctcaaa gcaattttga gtgacacagg aacacttaac ggctgacatg ggaattagct
     1021 tcacgctgcc gcaagcactc agggcgcaag ggctgctaaa ggaagcggaa cacgtagaaa
     1081 gccagtccgc agaaacggtg ctgaccccgg atgaatgtca gctgggaggc agaataaatg
     1141 atcatatcgt caattattac ctccacgggg agagcctgag caaactggcc tcaggcattt
     1201 gagaagcaca cggtcacact gcttccggta gtcaataaac cggtaagtag cgtatgcgct
     1261 cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt
     1321 tcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca tccaagcagc
     1381 aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc
     1441 agggcagtcg ccctaaaaca aagttaaaca tcatgaggga agcggtgatc gccgaagtat
     1501 cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg
     1561 ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt gatattgatt
     1621 tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc
     1681 ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca
     1741 ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg
     1801 gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg
     1861 atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg
     1921 cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct
     1981 taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt
     2041 tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg
     2101 actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg
     2161 cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg
     2221 tccactacgt gaaaggcgag atcaccaagg tagtcggcaa ataatgtcta acaattcgtt
     2281 caagccgacg gatctatgtc gggtgcggag aaagaggtaa tgaaatggca cctaggtat
//
