LOCUS       sequence-233806-        2371 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Plasmid donor for expression of proteins under the control of a 
            minimal HSP70 promoter in baculovirus/insect cell systems.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Monika Golas
  TITLE     Plasmids for expression of proteins in insect cells
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pGS-BacD-23005
FEATURES             Location/Qualifiers
     source          1..2371
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(20..41)
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     protein_bind    244..277
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      330..686
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     CDS             complement(1081..1740)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1655..1674
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(1741..1843)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     polyA_signal    2015..2149
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2039..2058)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2093..2112
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ttgtattcaa taattacttc ttggcagatt tcagtagttg cagttgattt acttggttgc
       61 tggttacttt taattgattc actttaactt gcactttact gcagattgtt tagcttgttc
      121 agctgcgctt gtttatttgc ttagctttcg cttagcgacg tgttcacttt gcttgtttga
      181 attgaattgt cgctccgtag acgaagcgcc tctatttata ctccggcgcc atgtgcctgg
      241 cagataactt cgtataatgt atgctatacg aagttatggt accgcggccg cgtagaggat
      301 ctgttgatca gcagttcaac ctgttgatag tacgtactaa gctctcatgt ttcacgtact
      361 aagctctcat gtttaacgta ctaagctctc atgtttaacg aactaaaccc tcatggctaa
      421 cgtactaagc tctcatggct aacgtactaa gctctcatgt ttcacgtact aagctctcat
      481 gtttgaacaa taaaattaat ataaatcagc aacttaaata gcctctaagg ttttaagttt
      541 tataagaaaa aaaagaatat ataaggcttt taaagctttt aaggtttaac ggttgtggac
      601 aacaagccag ggatgtaacg cactgagaag cccttagagc ctctcaaagc aattttgagt
      661 gacacaggaa cacttaacgg ctgacatggg aattagcttc acgctgccgc aagcactcag
      721 ggcgcaaggg ctgctaaagg aagcggaaca cgtagaaagc cagtccgcag aaacggtgct
      781 gaccccggat gaatgtcagc tgggaggcag aataaatgat catatcgtca attattacct
      841 ccacggggag agcctgagca aactggcctc aggcatttga gaagcacacg gtcacactgc
      901 ttccggtagt caataaaccg gtaaaccagc aatagacata agcggctatt taacgaccct
      961 gccctgaacc gacgaccggg tcgaatttgc tttcgaattt ctgccattca tccgcttatt
     1021 atcacttatt caggcgtagc aaccaggcgt ttaagggcac caataactgc cttaaaaaaa
     1081 ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac
     1141 atggaagcca tcacaaacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc
     1201 gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc
     1261 cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgagacga aaaacatatt
     1321 ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga
     1381 atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt
     1441 ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc
     1501 accgtctttc attgccatac ggaattccgg atgagcattc atcaggcggg caagaatgtg
     1561 aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat
     1621 atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg
     1681 ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat
     1741 tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct
     1801 tatttcatta tggtgaaagt tggaccctct tacgtgccga tcaacgtctc attttcgcca
     1861 aaagttggcc cagatctatg tcgggtgcgg agaaagaggt aatgaaatgg cacctagggg
     1921 ttatgatagt tattgctcag cggtggcagc agccaactca gcttcctttc gggctttgtt
     1981 agcagccgga tcttctaggc tcaagcagtg atcagatcca gacatgataa gatacattga
     2041 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
     2101 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
     2161 ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta
     2221 aaacctctac aaatgtggta tggctgatta tgatcctcta gtacttctcg acaagcttgt
     2281 cgagactgca ggctctagat tcgaaagcgg ccgcgactag tgagctcgtc gacgtaggcc
     2341 tttgaattcc gcgcgcttcg gaccgggatc c
//
