LOCUS       sequence-233867-       10156 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expresses mouse Type I procollagen alpha-2 chain (Col1a2) with 
            Gly610Cys mutation and mCerulean between signal sequence and exon 6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10156)
  AUTHORS   Omari S, Makareeva E, Roberts-Pilgrim A, Mirigian L, Jarnik M, Ott 
            C, Lippincott-Schwartz J, Leikin S
  TITLE     Noncanonical autophagy at ER exit sites regulates procollagen 
            turnover.
  JOURNAL   Proc Natl Acad Sci U S A. 2018 Oct 23;115(43):E10099-E10108. doi: 
            10.1073/pnas.1814552115. Epub 2018 Oct 4.
  PUBMED    30287488
REFERENCE   2  (bases 1 to 10156)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1814552115"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2018-10-23- 23"; volume: "115"; issue: "43"; pages: 
            "E10099-E10108"
COMMENT     Sequence Label: mCerulean-pro-alpha-2(I)-G610C
FEATURES             Location/Qualifiers
     source          1..10156
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(805..807,808..1518)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(847..868)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1108..1127)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     complement(5513..5530)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5519..5743
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5789..6217
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5876..5895)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6086..6107
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6226..6246)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6231..6560
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6411..6546
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6473..6492
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6627..7421
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6681..6700)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7291..7310
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7595..7716
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7632..7651)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7686..7705
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7765..7781)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7765..7781)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7778..7800)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7789..7805
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7813..7819,7820..7837,7838..7843))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7858..7879
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7996..8013)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8167..8755)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8247..8266)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8926..9717,9718..9786))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9549..9568
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9787..9891)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9966..9985)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagcgttt aaacttaagc ttggtaccga gctcggatcc actagtccag tgtggtggaa
      721 ttcgtagaca tgctcagctt tgtggatacg cggactctgt tgctgcttgc agtaacttcg
      781 tgcctagcaa catgccaaga tatcgtgagc aagggcgagg agctgttcac cggggtggtg
      841 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
      901 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
      961 ctgcccgtgc cctggcccac cctcgtgacc accctgacct ggggcgtgca gtgcttcgcc
     1021 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     1081 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     1141 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     1201 gacggcaaca tcctggggca caagctggag tacaacgcca tcagcgacaa cgtctatatc
     1261 accgccgaca agcagaagaa cggcatcaag gccaacttca agatccgcca caacatcgag
     1321 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     1381 gtgctgctgc ccgacaacca ctacctgagc acccagtcca agctgagcaa agaccccaac
     1441 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     1501 atggacgagc tgtacaagga tatctattct gacaaaggag tttcatctgg ccctggacca
     1561 atgggtttaa tgggacccag aggccctcct ggtgctgttg gagcccctgg ccctcaaggt
     1621 ttccaaggac ctgctggtga acctggtgag cctggtcaaa cgggtcctgc aggtccccgt
     1681 ggtccagctg gctctcctgg caaggctggt gaggatggtc accctggaaa acccggaaga
     1741 cctggggaga gaggagtcgt tggaccacag ggtgctcgtg gtttccccgg gactcctgga
     1801 cttcctggct tcaaaggcgt gaaaggacac agtggtatgg atggattaaa gggacagcct
     1861 ggtgcacagg gtgtgaaggg tgaacccggt gcccctggag agaatggaac tccaggtcaa
     1921 gcaggagccc gagggcttcc tggtgagaga gggcgtgtcg gagctcctgg tccagctggt
     1981 gcccgaggca gtgatggaag cgttggcccc gtcggccctg ctggtcccat tgggtctgct
     2041 ggaccccctg gtttcccagg tgctcctggt cccaagggtg aacttgggcc cgttggtaac
     2101 cctggccctg ctggtcctgc tggtccccgt ggtgaagtgg gtcttccagg tctttctggc
     2161 cctgttggac ctcctggcaa ccctggaaca aatgggctca ctggcgccaa gggtgctact
     2221 ggactccctg gtgtcgctgg ggctcccggt ctccctggcc cccgtggtat tcctggcccc
     2281 gctggtgctg ctggtgctac aggtgccaga ggacttgttg gtgagcctgg tcctgctggc
     2341 tccaaaggag aatccggtaa caagggtgag cctggctctg ttggagccca aggtcctcct
     2401 ggtcccagcg gtgaagaagg aaagagaggg tctcccggag aagctggatc tgctggccct
     2461 gcagggcctc cagggcttag aggcagccct ggttctcgag gtcttcctgg agctgatggc
     2521 agagctggtg taatgggtcc tcctggcaat cgtggttcaa ccggccctgc tggaatccga
     2581 ggtcctaatg gagatgccgg tcgacctggg gaacctggtc tcatgggacc cagaggtctt
     2641 cctgggtctc ctggaaatgt tggcccatct ggtaaagaag gccctgtggg tctccctggc
     2701 attgatggca gacctggccc aatcggccca gctggaccaa gaggtgaagc tggcaacatt
     2761 ggattccctg gacccaaagg cccctctggt gatcctggca aacctggtga gagaggacac
     2821 cccggtcttg ctggtgctcg gggagctcca ggacccgatg gcaacaatgg agctcagggc
     2881 ccccctggac cccagggtgt tcaaggtggc aaaggtgaac aaggccctgc tggtcctcct
     2941 ggcttccagg gtctccctgg tccctcaggt actactggag aagttggcaa gcccggagaa
     3001 aggggtcttc ctggtgaatt cggtctccct ggtcctgctg gtccaagagg agaacgtggt
     3061 accccgggtg agagtggagc tgctggccct tctggtccta ttggaagccg aggtcccagt
     3121 ggagccccag ggcctgatgg aaacaagggt gaagctggtg cagtcggtgc tccaggcagt
     3181 gctggtgcct ctgggcctgg tgggcttcca ggagagaggg gtgctgctgg catacctggg
     3241 ggcaaaggag aaaagggtga aactggtctc cgaggtgaca ctggcaacac tggtagagat
     3301 ggtgctcgtg gcattcctgg tgctgtaggt gcccctggtc ctgctggggc ctcaggtgac
     3361 cggggtgaag ctggtgctgc ctgtccttct ggcccagctg gtcctcgggg tagccctggt
     3421 gaacgtggtg aagttggccc tgctggcccc aatggatttg ctggtcctgc tggtgctgct
     3481 ggccaacccg gtgctaaagg agagaaggga accaaagggc ctaagggtga aaatggcatt
     3541 gttggtccaa ccgggtctgt tggagctgct ggcccatctg gtcctaatgg cccacctggt
     3601 cctgttggaa gtcgtggtga tggagggccc cctggtatga ctggcttccc tggtgctgct
     3661 ggacggactg gtccccccgg acccagtggt attgctggcc cccctggccc ccctggtgct
     3721 gctggaaagg aagggattcg tggtccccgt ggcgaccaag gtccagttgg ccgaactgga
     3781 gaaacaggtg catctggacc ccctggattt gttggtgaaa agggtccctc tggagaacct
     3841 ggtaccgcag gagcccctgg taccgcaggt cctcagggtc ttcttggtgc tcccggtatt
     3901 ctgggtctcc ctggctctag aggtgaacgt ggtctgccag gtattgctgg tgctctgggt
     3961 gaacctggtc ctctgggcat ctcaggccct cctggtgccc gtggtccccc tggtgctgtg
     4021 ggcagccctg gagttaatgg tgcccctggt gaagctggtc gtgatggtaa ccctggcagc
     4081 gatggtcccc caggtcgtga tggtcagcct ggacacaagg gagagcgtgg ttaccctggc
     4141 agcattggtc cgactggtgc tgcaggtgcc cctggtcctc acggttctgt gggtcctgct
     4201 gggaaacatg gaaaccgagg tgaacctggt cctgccggtt ctgttggtcc tgttggtgct
     4261 gttggtccaa gaggtcctag tggtcctcaa ggcatccgag gtgacaaagg agaacctggt
     4321 gataaagggc acaggggtct tcctggctta aaaggataca gtggattgca gggtcttcct
     4381 ggtcttgctg gcctacatgg tgaccaagga gctcctggtc ctgtgggtcc tgctggtccc
     4441 aggggtcctg ctgggccttc tggacccgtt ggcaaagatg gtagatctgg gcaacctggc
     4501 ccagtcgggc ctgctggtgt tcgtggttct cagggtagcc aaggtcccgc tggtccccct
     4561 ggtccccctg gacctcctgg tcctcctggt gtcagcggag gtggctatga ctttggtttt
     4621 gaaggagact tctacagggc tgaccagcct cgctcccagc cttcactcag acccaaggac
     4681 tatgaagttg atgcaactct gaaatctctc aataaccaga ttgagaccct tctcactcct
     4741 gaaggctcta gaaagaaccc tgctcgcacg tgccgggact taagactcag ccacccagag
     4801 tggaacagcg attactactg gattgaccct aaccaaggat gtactatgga tgccatcaaa
     4861 gtgtactgtg atttctctac tggtgaaacc tgcatccagg cccaacctgt aaacacccca
     4921 gcgaagaact catacagccg cgcccaggcc aacaagcatg tctggttagg agagactatc
     4981 aatggtggca gccagtttga atacaacgta gaaggggtgt cctccaagga aatggcaact
     5041 cagctcgcct tcatgcgcct gctagccaac cgtgcttctc agaacatcac ctaccactgc
     5101 aagaacagca ttgcgtacct ggatgaggag acgggcagct tgaacaaggc tgtgcttctg
     5161 cagggttcca acgatgttga acttgttgct gagggcaaca gcaggttcac ctactctgtc
     5221 ctagtcgatg gctgctccaa aaagacaaat gaatggggca agacaatcat tgaatacaaa
     5281 acaaataagc catctcgcct gccgttcctt gacattgcac ctctggacat tggtggtgct
     5341 gaccaagaat tccgtgtgga ggttggcccc gtctgtttca aataagtgaa ctcaacataa
     5401 attaaaaacc aaaaacccct gaaaaaactt tctctttgcc gtttcctcct ttaaaaaaaa
     5461 aaaaaagggc ggccgctcga gtctagaggg cccgtttaaa cccgctgatc agcctcgact
     5521 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     5581 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     5641 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     5701 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
     5761 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg
     5821 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     5881 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     5941 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     6001 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     6061 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     6121 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     6181 aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt
     6241 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     6301 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     6361 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     6421 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     6481 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg
     6541 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc
     6601 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc
     6661 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
     6721 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
     6781 acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca
     6841 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
     6901 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
     6961 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
     7021 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
     7081 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
     7141 ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
     7201 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
     7261 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
     7321 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
     7381 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga
     7441 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt
     7501 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
     7561 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
     7621 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     7681 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
     7741 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     7801 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     7861 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     7921 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     7981 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     8041 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     8101 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     8161 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     8221 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     8281 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     8341 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     8401 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     8461 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     8521 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     8581 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     8641 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     8701 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     8761 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     8821 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     8881 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     8941 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     9001 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     9061 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     9121 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     9181 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     9241 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     9301 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     9361 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     9421 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     9481 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     9541 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     9601 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     9661 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     9721 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     9781 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     9841 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     9901 ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact
     9961 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg
    10021 ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc
    10081 gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg
    10141 gccagatata cgcgtt
//
