LOCUS       sequence-233929-        4587 bp    DNA     circular SYN 15-APR-2025
DEFINITION  QUAS::CFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Mao S, Qi Y, Zhu H, Huang X, Zou Y, Chi T
  TITLE     A Tet/Q Hybrid System for Robust and Versatile Control of Transgene 
            Expression in C. elegans.
  JOURNAL   iScience. 2019 Jan 25;11:224-237. doi: 10.1016/j.isci.2018.12.023. 
            Epub 2018 Dec 27.
  PUBMED    30634168
REFERENCE   2  (bases 1 to 4587)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2019-01-25"; volume: "11:224-237. doi"; pages: " 
            10.1016/j.isci.2018.12.023. Epub 2018 Dec 27"
COMMENT     Sequence Label: TC812
FEATURES             Location/Qualifiers
     source          1..4587
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2013..2035)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2135..2154
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2232..2250)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2318..2422
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2423..2491,2492..3283)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2641..2660)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3454..4042
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3943..3962
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4196..4213
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4330..4351
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4366..4371,4372..4389,4390..4396)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4404..4420
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4409..4431
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4428..4444
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4428..4444
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aattcggatc cgggtaatcg cttatcctcg gataaacaat tatcctcacg ggtaatcgct
       61 tatccgctcg ggtaatcgct tatcctcggg taatcgctta tccttaagct tgatatctct
      121 aggatcgatt ttttgcaaat tacgagcgtt gtagggggcg gagcgatagg tcctataggt
      181 tttggtatat catcattcat tcattcattg gtacattcat ttacccacct tcctctttct
      241 gagcttctct ggagttctgt gcttcctttt tcccttatct ttatactgta atttttaact
      301 ttcagccggt agaaaaaatg gtttctaagg gagaagaact tttcactgga gttgttccaa
      361 ttcttgttga attggatgga gatgttaacg gacataagtt ctcagtttct ggagaaggag
      421 aaggagatgc tacatacgga aaacttactt tgaagttcat ctgtacaact ggaaaattgc
      481 cagttccatg ggtaagttta aacatatata tactaactaa ccctgattat ttaaattttc
      541 agccaacact tgttacaact ttgacttggg gagttcaatg ctttgcaaga tacccagatc
      601 atatgaagca acatgatttc tttaagtcag ctatgccaga aggatacgtt caagaaagaa
      661 caattttctt taaggatgat ggaaactaca agacacgagc agaggttaag ttcgaaggag
      721 atactcttgt taacagaatc gaattgaagg gaatcgattt taaagaagat ggaaacatcc
      781 ttggacataa gttggaatac aatgctatct ctgataacgt ttacatcact gcagataagc
      841 aaaagaatgg aattaaagct aacttcaaga ttcgacataa tattgaagat ggatcagttc
      901 aacttgctga tcattaccaa caaaacacac caattggaga tggaccagtt cttttgccag
      961 ataaccatta cctttcaact caatctaagt tgtcaaagga tccaaacgaa aagagagatc
     1021 atatggttct tttggaattc gttacagctg caggaattac tcttggaatg gatgaattgt
     1081 ataaataagc attcgtagaa ttccaactga gcgccggtcg ctaccattac caacttgtct
     1141 ggtgtcaaaa ataatagggg ccgctgtcat cagagtaagt ttaaactgag ttctactaac
     1201 taacgagtaa tatttaaatt ttcagcatct cgcgcccgtg cctctgactt ctaagtccaa
     1261 ttactcttca acatccctac atgctctttc tccctgtgct cccaccccct atttttgtta
     1321 ttatcaaaaa acttctctta atttctttgt tttttagctt cttttaagtc acctctaaca
     1381 atgaaattgt gtagattcaa aaatagaatt aattcgtaat aaaaagtcga aaaaaattgt
     1441 gctccctccc cccattaata ataattctat cccaaaatct acacaatgtt ctgtgtacac
     1501 ttcttatgtt ttttacttct gataaatttt tttgaaacat catagaaaaa accgcacaca
     1561 aaatacctta tcatatgtta cgtttcagtt tatgaccgca atttttattt cttcgcacgt
     1621 ctgggcctct catgacgtca aatcatgctc atcgtgaaaa agttttggag tatttttgga
     1681 atttttcaat caagtgaaag tttatgaaat taattttcct gcttttgctt tttggggttt
     1741 cccctattgt ttgtcaagat ttcgaggacg gcgtttttct tgctaaaatc acaagtattg
     1801 atgagcacga tgcaagaaag atcggaagaa ggtttgggtt tgaggctcag tggaaggtga
     1861 gtagaagttg ataatttgaa agtggagtag tgtctatggg gtttttgcct taaatgacag
     1921 aatacattcc caatatacca aacataactg tttcctacta gtcggccgta cgggcccttt
     1981 cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
     2041 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     2101 ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga
     2161 gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
     2221 cggccttaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     2281 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     2341 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     2401 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     2461 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     2521 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     2581 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     2641 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     2701 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     2761 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     2821 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     2881 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     2941 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     3001 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     3061 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     3121 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     3181 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     3241 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     3301 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     3361 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     3421 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     3481 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     3541 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     3601 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     3661 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     3721 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     3781 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     3841 tgagcattga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     3901 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     3961 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     4021 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     4081 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     4141 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     4201 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     4261 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
     4321 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
     4381 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
     4441 tgaccatgat tacgccaagc tgtaagttta aacatgatct tactaactaa ctattctcat
     4501 ttaaattttc agagcttaaa aatggctgaa atcactcaca acgatggata cgctaacaac
     4561 ttggaaatga aataagcttg catgccc
//
