LOCUS       sequence-234013-        2630 bp    DNA     circular SYN 15-APR-2025
DEFINITION  ssRNA expression for dsRNA production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2630)
  TITLE     Petr Svoboda lab unpublished
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: U6-Mos-as
FEATURES             Location/Qualifiers
     source          1..2630
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        77..325
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     77..97
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     248..267
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(626..644)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        712..816
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(817..885,886..1677)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1035..1054)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1848..2436
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2337..2356
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2590..2607
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
       61 cgactggaaa gcaattgagg gcctatttcc catgattcct tcatatttgc atatacgata
      121 caaggctgtt agagagataa ttggaattaa tttgactgta aacacaaaga tattagtaca
      181 aaatacgtga cgtagaaagt aataatttct tgggtagttt gcagttttaa aattatgttt
      241 taaaatggac tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct tggctttata
      301 tatcttgtgg aaaggacgaa acaccgcggc cgctaacaac atctagggaa tacttgaggc
      361 acttccccaa actcggttgt tctctgcagc tgagaggctc cggtgagcgg gtggcaccgt
      421 agatgacttg gtgtagagtc acgttgcccc caaactccat gattatggta cctaggctgt
      481 tggagtcttc gggcgtgcgc gtgctggcag ccacaacccg aactatgttg tcgtggcgta
      541 gtcttgcaat gttcagttca gcccagaaac tccgctggga tgcacgtagg tccttggtgc
      601 gcggccgctt ttttctgcag gggcggcctc gtgatacgcc tatttttata ggttaatgtc
      661 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
      721 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
      781 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
      841 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
      901 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
      961 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     1021 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     1081 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     1141 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     1201 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     1261 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     1321 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     1381 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     1441 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     1501 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     1561 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     1621 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     1681 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     1741 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     1801 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     1861 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     1921 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     1981 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     2041 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     2101 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     2161 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     2221 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     2281 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     2341 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     2401 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     2461 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     2521 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     2581 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
//
