LOCUS       sequence-23408-e         799 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Takemaru K, Yamaguchi S, Lee YS, Zhang Y, Carthew RW, Moon RT
  TITLE     Chibby, a nuclear beta-catenin-associated antagonist of the 
            Wnt/Wingless pathway.
  JOURNAL   Nature. 2003 Apr 24. 422(6934):905-9.
  PUBMED    12712206
REFERENCE   2  (bases 1 to 799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2003 Apr 24. 422(6934):905-9."
COMMENT     Sequence Label: XE225 mouse Chibby-pCS2+
FEATURES             Location/Qualifiers
     source          1..799
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    431..565
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(455..474)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     509..528
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(678..698)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(678..696)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(717..733)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(717..733)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(730..752)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    741..757
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 gnttnngaca ngcctctctt tggcagcata ttcagcccaa agaagacccc tcctcggaag
       61 tcggcctctc tctccaacct acattcttta gatcggtcaa cgcgggaact ggagctgggg
      121 ctcgactatg gaactcctac catgaacctg gcggggcaga gcctgaagtt tgaaaacggc
      181 cagtgggtgg cagactccgt gattagtggg ggtgtggacc ggagggaaac tcagcgcctg
      241 cgcaaacgaa accaacagtt agaagaagaa aacaatctcc tgcggctgaa agtggacatt
      301 ctgctggaca tgctttcaga aaccactgct gagtcccacc taaaggacaa agagctggat
      361 gaactgaagg ttaccaaccg caggaggaag tgactcgagc ctctagaact atagtgagtc
      421 gtattacgta gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag
      481 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
      541 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
      601 tcagggggag gtgtgggagg ttttttaatt cgcggccgcg gcgccaatgc attgggcccg
      661 gtacccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca
      721 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cncacaacat acgagccggg
      781 agcataaatg aagccgggg
//
