LOCUS       sequence-234091-       12166 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expresses disordered protein TAF15(1-208) fused with fluorescent 
            protein miRFP670 and optogenetic protein Cry2WT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12166)
  AUTHORS   Shin Y, Chang YC, Lee DSW, Berry J, Sanders DW, Ronceray P, Wingreen
            NS, Haataja M, Brangwynne CP
  TITLE     Liquid Nuclear Condensates Mechanically Sense and Restructure the 
            Genome.
  JOURNAL   Cell. 2018 Nov 29;175(6):1481-1491.e13. doi: 
            10.1016/j.cell.2018.10.057.
  PUBMED    30500535
REFERENCE   2  (bases 1 to 12166)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.10"; journalName: "Cell"; date: "2018-11-29- 
            29"; volume: "175"; issue: "6"; pages: "1481-1491.e13"
COMMENT     Sequence Label: pHR-TAF15N-miRFP670-Cry2WT
FEATURES             Location/Qualifiers
     source          1..12166
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    43..160
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        313..720
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             1421..2365
                     /codon_start=1
                     /product="bright monomeric near-infrared fluorescent 
                     protein engineered from a bacterial phytochrome, with an 
                     emission maximum of 670 nm (Shcherbakova et al., 2016)"
                     /label=miRFP670
                     /label=ORF frame 1
                     /translation="MVAGHASGSPAFGTASHSNCEHEEIHLAGSIQPHGALLVVSEHDH
                     RVIQASANAAEFLNLGSVLGVPLAEIDGDLLIKILPHLDPTAEGMPVAVRCRIGNPSTE
                     YCGLMHRPPEGGLIIELERAGPSIDLSGTLAPALERIRTAGSLRALCDDTVLLFQQCTG
                     YDRVMVYRFDEQGHGLVFSECHVPGLESYFGNRYPSSTVPQMARQLYVRQRVRVLVDVT
                     YQPVPLEPRLSPLTGRDLDMSGCFLRSMSPCHLQFLKDMGVRATLAVSLVVGGKLWGLV
                     VCHHYLPRFIRFELRAICKRLAERIATRITALES"
     CDS             2378..3871
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     misc_feature    4023..4611
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4076..4096)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4494..4505)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4614..4630)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4615..4631)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4721..4954
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4979..4997)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4980..4997)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5287..5306)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5520..5539)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5639..5661
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5699..5717)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5785..5889
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5890..5958,5959..6750)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6108..6127)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6921..7509
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7410..7429
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7663..7680
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7750..7770)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7755..8084
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7935..8070
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          9218..9283
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9413..9433
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9858..9992
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9895..9914)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9949..9968
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10160..10793
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10840..10965
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11462..11695
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11880..11924
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 cggatctcga cggtatcgcc aaatggcagt attcatccac aattttaaaa gaaaaggggg
       61 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
      121 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
      181 cagagatcca gtttggatcg ataagcttga tatcgaattc ctgcagcccc gataaaataa
      241 aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc
      301 aagctagctg cagtaacgcc attttgcaag gcatggaaaa ataccaaacc aagaatagag
      361 aagttcagat caagggcggg tacatgaaaa tagctaacgt tgggccaaac aggatatctg
      421 cggtgagcag tttcggcccc ggcccggggc caagaacaga tggtcaccgc agtttcggcc
      481 ccggcccgag gccaagaaca gatggtcccc agatatggcc caaccctcag cagtttctta
      541 agacccatca gatgtttcca ggctccccca aggacctgaa atgaccctgc gccttatttg
      601 aattaaccaa tcagcctgct tctcgcttct gttcgcgcgc ttctgcttcc cgagctctat
      661 aaaagagctc acaacccctc actcggcgcg ccagtcctcc gacagactga gtcgcccggg
      721 ggggatctgg agctctcgag aattctcacg cgacagtgga tccggagcta ccatgtcgga
      781 ttctggaagt tacggtcagt ctgggggtga gcagcaaagt tattctacct atggaaatcc
      841 aggcagccaa ggctatggac aagcatcaca aagctattct ggctatgggc aaacgactga
      901 ttcctcttat ggacagaact acagcggtta ctccagttat ggacaaagtc agtcaggtta
      961 ttcacagtcc tatggtggtt atgagaatca aaagcagagc tcatatagcc agcaaccata
     1021 taataaccag ggacagcagc aaaacatgga atcatcagga agccaaggtg gaagagcacc
     1081 ttcctatgac cagccagact atggtcaaca agattcatat gaccagcagt caggctatga
     1141 tcaacatcaa ggctcatatg atgagcagtc aaattatgat cagcagcatg attcctatag
     1201 tcaaaaccag cagtcctatc attcacaaag ggaaaactac agccaccaca cacaagatga
     1261 ccgtcgtgat gtgagtaggt atggagaaga taatagagga tatggcgggt cacagggagg
     1321 aggtagaggg cgtgggggat atgacaagga tggaagaggt cctatgacag gatcaagtgg
     1381 tggtgaccgc ggtggcggag gcggctccgg aggcggaagc atggtagcag gtcatgcctc
     1441 tggcagcccc gcattcggga ccgcctctca ttcgaattgc gaacatgaag agatccacct
     1501 cgccggctcg atccagccgc atggcgcgct tctggtcgtc agcgaacatg atcatcgcgt
     1561 catccaggcc agcgccaacg ccgcggaatt tctgaatctc ggaagcgtac tcggcgttcc
     1621 gctcgccgag atcgacggcg atctgttgat caagatcctg ccgcatctcg atcccaccgc
     1681 cgaaggcatg ccggtcgcgg tgcgctgccg gatcggcaat ccctctacgg agtactgcgg
     1741 tctgatgcat cggcctccgg aaggcgggct gatcatcgaa ctcgaacgtg ccggcccgtc
     1801 gatcgatctg tcaggcacgc tggcgccggc gctggagcgg atccgcacgg cgggttcact
     1861 gcgcgcgctg tgcgatgaca ccgtgctgct gtttcagcag tgcaccggct acgaccgggt
     1921 gatggtgtat cgtttcgatg agcaaggcca cggcctggta ttctccgagt gccatgtgcc
     1981 tgggctcgaa tcctatttcg gcaaccgcta tccgtcgtcg actgtcccgc agatggcgcg
     2041 gcagctgtac gtgcggcagc gcgtccgcgt gctggtcgac gtcacctatc agccggtgcc
     2101 gctggagccg cggctgtcgc cgctgaccgg gcgcgatctc gacatgtcgg gctgcttcct
     2161 gcgctcgatg tcgccgtgcc atctgcagtt cctgaaggac atgggcgtgc gcgccaccct
     2221 ggcggtgtcg ctggtggtcg gcggcaagct gtggggcctg gttgtctgtc accattatct
     2281 gccgcgcttc atccgtttcg agctgcgggc gatctgcaaa cggctcgccg aaaggatcgc
     2341 gacgcggatc accgcgcttg agagcgcggc cacgcgtatg aagatggaca aaaagactat
     2401 agtttggttt agaagagacc taaggattga ggataatcct gcattagcag cagctgctca
     2461 cgaaggatct gtttttcctg tcttcatttg gtgtcctgaa gaagaaggac agttttatcc
     2521 tggaagagct tcaagatggt ggatgaaaca atcacttgct cacttatctc aatccttgaa
     2581 ggctcttgga tctgacctca ctttaatcaa aacccacaac acgatttcag cgatcttgga
     2641 ttgtatccgc gttaccggtg ctacaaaagt cgtctttaac cacctctatg atcctgtttc
     2701 gttagttcgg gaccataccg taaaggagaa gctggtggaa cgtgggatct ctgtgcaaag
     2761 ctacaatgga gatctattgt atgaaccgtg ggagatatac tgcgaaaagg gcaaaccttt
     2821 tacgagtttc aattcttact ggaagaaatg cttagatatg tcgattgaat ccgttatgct
     2881 tcctcctcct tggcggttga tgccaataac tgcagcggct gaagcgattt gggcgtgttc
     2941 gattgaagaa ctagggctgg agaatgaggc cgagaaaccg agcaatgcgt tgttaactag
     3001 agcttggtct ccaggatgga gcaatgctga taagttacta aatgagttca tcgagaagca
     3061 gttgatagat tatgcaaaga acagcaagaa agttgttggg aattctactt cactactttc
     3121 tccgtatctc catttcgggg aaataagcgt cagacacgtt ttccagtgtg cccggatgaa
     3181 acaaattata tgggcaagag ataagaacag tgaaggagaa gaaagtgcag atctttttct
     3241 taggggaatc ggtttaagag agtattctcg gtatatatgt ttcaacttcc cgtttactca
     3301 cgagcaatcg ttgttgagtc atcttcggtt tttcccttgg gatgctgatg ttgataagtt
     3361 caaggcctgg agacaaggca ggaccggtta tccgttggtg gatgccggaa tgagagagct
     3421 ttgggctacc ggatggatgc ataacagaat aagagtgatt gtttcaagct ttgctgtgaa
     3481 gtttcttctc cttccatgga aatggggaat gaagtatttc tgggatacac ttttggatgc
     3541 tgatttggaa tgtgacatcc ttggctggca gtatatctct gggagtatcc ccgatggcca
     3601 cgagcttgat cgcttggaca atcccgcgtt acaaggcgcc aaatatgacc cagaaggtga
     3661 gtacataagg caatggcttc ccgagcttgc gagattgcca actgaatgga tccatcatcc
     3721 atgggacgct cctttaaccg tactcaaagc ttctggtgtg gaactcggaa caaactatgc
     3781 gaaacccatt gtagacatcg acacagctcg tgagctacta gctaaagcta tttcaagaac
     3841 ccgtgaagca cagatcatga tcggagcagc agcccgggat ccaccggtcg ccaccggttc
     3901 agggagcgga tccggctcag ggagtgcggc cgcaactccc acctgcaaca tgcgtgactg
     3961 actgaggccg cgactctaga gtcgacctgc aggcatgcaa gcttgatatc aagcttatcg
     4021 ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     4081 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     4141 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     4201 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     4261 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     4321 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     4381 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
     4441 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     4501 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     4561 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc
     4621 gtcgacctcg agggaattaa ttcgagctcg gtacctttaa gaccaatgac ttacaaggca
     4681 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     4741 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     4801 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     4861 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     4921 agaccctttt agtcagtgtg gaaaatctct agcagcatct agaattaatt ccgtgtattc
     4981 tatagtgtca cctaaatcgt atgtgtatga tacataaggt tatgtattaa ttgtagccgc
     5041 gttctaacga caatatgtac aagcctaatt gtgtagcatc tggcttactg aagcagaccc
     5101 tatcatctct ctcgtaaact gccgtcagag tcggtttggt tggacgaacc ttctgagttt
     5161 ctggtaacgc cgtcccgcac ccggaaatgg tcagcgaacc aatcagcagg gtcatcgcta
     5221 gccagatcct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg
     5281 ctggcgccta tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca
     5341 tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg
     5401 ccatctcctt gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac
     5461 tgggctgctt cctaatgcag gagtcgcata agggagagcg tcgaatggtg cactctcagt
     5521 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
     5581 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     5641 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc
     5701 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     5761 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     5821 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     5881 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     5941 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     6001 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     6061 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     6121 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     6181 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     6241 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     6301 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     6361 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     6421 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     6481 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     6541 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     6601 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     6661 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     6721 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     6781 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     6841 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     6901 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     6961 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     7021 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     7081 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     7141 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     7201 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     7261 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     7321 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     7381 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     7441 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     7501 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     7561 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     7621 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     7681 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     7741 tgcagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     7801 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     7861 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     7921 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     7981 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     8041 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctt ggacacaaga
     8101 caggcttgcg agatatgttt gagaatacca ctttatcccg cgtcagggag aggcagtgcg
     8161 taaaaagacg cggactcatg tgaaatactg gtttttagtg cgccagatct ctataatctc
     8221 gcgcaaccta ttttcccctc gaacactttt taagccgtag ataaacaggc tgggacactt
     8281 cacatgagcg aaaaatacat cgtcacctgg gacatgttgc agatccatgc acgtaaactc
     8341 gcaagccgac tgatgccttc tgaacaatgg aaaggcatta ttgccgtaag ccgtggcggt
     8401 ctgtaccggg tgcgttactg gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta
     8461 tttccagcta cgatcacgac aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg
     8521 atggcgaagg cttcatcgtt attgatgacc tggtggatac cggtggtact gcggttgcga
     8581 ttcgtgaaat gtatccaaaa gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc
     8641 cgctggttga tgactatgtt gttgatatcc cgcaagatac ctggattgaa cagccgtggg
     8701 atatgggcgt cgtattcgtc ccgccaatct ccggtcgcta atcttttcaa cgcctggcac
     8761 tgccgggcgt tgttcttttt aacttcaggc gggttacaat agtttccagt aagtattctg
     8821 gaggctgcat ccatgacaca ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa
     8881 catcctgaaa cctcgacgct agtccgccgc tttaatcacg gcgcacaacc gcctgtgcag
     8941 tcggcccttg atggtaaaac catccctcac tggtatcgca tgattaaccg tctgatgtgg
     9001 atctggcgcg gcattgaccc acgcgaaatc ctcgacgtcc aggcacgtat tgtgatgagc
     9061 gatgccgaac gtaccgacga tgatttatac gatacggtga ttggctaccg tggcggcaac
     9121 tggatttatg agtgggcccc ggatctttgt gaaggaacct tacttctgtg gtgtgacata
     9181 attggacaaa ctacctacag agatttaaag ctctaaggta aatataaaat ttttaagtgt
     9241 ataatgtgtt aaactactga ttctaattgt ttgtgtattt tagattccaa cctatggaac
     9301 tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa aacctgtttt gctcagaaga
     9361 aatgccatct agtgatgatg aggctactgc tgactctcaa cattctactc ctccaaaaaa
     9421 gaagagaaag gtagaagacc ccaaggactt tccttcagaa ttgctaagtt ttttgagtca
     9481 tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc
     9541 tgcactgcta tacaagaaaa ttatggaaaa atattctgta acctttataa gtaggcataa
     9601 cagttataat cataacatac tgttttttct tactccacac aggcatagag tgtctgctat
     9661 taataactat gctcaaaaat tgtgtacctt tagcttttta atttgtaaag gggttaataa
     9721 ggaatatttg atgtatagtg ccttgactag agatcataat cagccatacc acatttgtag
     9781 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
     9841 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     9901 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     9961 aactcatcaa tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc
    10021 atcccaaact tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac
    10081 tctaaacctg tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat
    10141 gtactacaaa cttagtagtt ggaagggcta attcactccc aaagaagaca agatatcctt
    10201 gatctgtgga tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg
    10261 ccaggggtca gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca
    10321 gataaggtag aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg
    10381 catgggatgg atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca
    10441 tttcatcacg tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct
    10501 tgctacaagg gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg
    10561 gagtggcgag ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct
    10621 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
    10681 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
    10741 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
    10801 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
    10861 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
    10921 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
    10981 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
    11041 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
    11101 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
    11161 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
    11221 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
    11281 agtaagacca ccgcacagca agcggccggc cgctgatctt cagacctgga ggaggagata
    11341 tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag
    11401 gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa
    11461 taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa
    11521 tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt
    11581 tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc
    11641 agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga
    11701 tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga
    11761 gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa
    11821 ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa
    11881 agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca
    11941 taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt
    12001 taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat
    12061 tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag
    12121 aagaaggtgg agagagagac agagacagat ccattcgatt agtgaa
//
