LOCUS       sequence-234131-        8415 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8415)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 8415)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_194920-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..8415
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1086..1105
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1128..1137
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3573..3590
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3642..3697
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3696..3715)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5933..5951)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6019..6123
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6124..6192,6193..6984)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6342..6361)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7155..7743
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7644..7663
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7897..7914
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8031..8052
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8067..8072,8073..8090,8091..8097)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8105..8121
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8110..8132
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8129..8145
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8129..8145
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcctcac tctcttccgc atcgctgtct gcgagggcca gctgttgggc tcgcggttga
      661 ggacaaactc ttcgcggtct ttccagtact cttggatcgg aaacccgtcg gcctccgaac
      721 ggtactccgc caccgaggga cctgagcgag tccgcatcga ccggatcgga aaacctctcg
      781 agaaaggcgt ctaaccagtc acagtcgcaa ggtaggctga gcaccgtggc gggcggcagc
      841 gggtggcggt cggggttgtt tctggcggag gtgctgctga tgatgtaatt aaagtaggcg
      901 gtcttgagac ggcggatggt cgaggtgagg tgtggcaggc ttgagatcca gctgttgggg
      961 tgagtactcc ctctcaaaag cgggcattac ttctgcgcta agattgtcag tttccaaaaa
     1021 cgaggaggat ttgatattca cctggcccga tctggccata cacttgagtg acaatgacat
     1081 ccactttgcc tttctctcca caggtgtcca ctcccaggtc caagtttgcc accatggagt
     1141 ttcagaccca ggtactcatg tccctgctgc tctgcatgtc tggtgcggcc gccgacaaga
     1201 acatcatcct gaacaacaac cccagcgact tctaccagca gctgaacgcc tggcccgact
     1261 tccagctggc cgccttcacc gacctgaacg ccgaccggcg gatggacatc atcttcgcca
     1321 acggcgacgg cagcaagctg ttcgccgtgc tgcagagcga caacaagggc ttccagagca
     1381 ccagcagcat cctgagcagc gtgaagttca gcccccctgt ggaaatcatc ggcaacgtgc
     1441 tgcccaagcc cgtgcggggc gtggccacca gcgatttcaa cggcgacagc cggatcgacc
     1501 tgctgctggt cacaggcagc agcgacggcg gacccttcga cgtgtacgtg gcctacggcc
     1561 tgagcggcga gaaccggggc aagttcgaca gccccaagtt cctggacacc ttcaccagcc
     1621 agcccctcgt gtgcgacctg aacagcgaca tgatcgccga tgtgtacggc gaggtgtccg
     1681 gccacagagt ggcctacatc ggcggcaaca agctgaccaa gctgagcgcc acctacagcg
     1741 gccccagctg gtccagactg ggctacagcg ccttcggcga catcaatggc gacggcgtgc
     1801 ccgacatcgt gatcctggtg gacaacgaga accggcagca gctgcaggtc atcctgcgga
     1861 cccagagcag catcggcagc ctgcccgatg tggccaacgc cgagctgatc gacctggacc
     1921 ccaagctgct gagcaccccc cagagcgtgc tgggcctgtt cgtgctgaac gacttcgact
     1981 acgacggcct gattgatctg ctgctgcccg tgtgccggga cgccaagtgc ctggatacca
     2041 gctccatcaa catgtacagc ttccgggacc ggaagtggtc ccccgtgaac gtgatctggg
     2101 agcccagcaa cgtgaaagaa aacaccatgc ggtggtccct gaccagcacc cctcagatca
     2161 agagcggcct gctgaccagc tccctgatcg gacccgctgt gggcgacgtg aacatggacg
     2221 gcaagcccga tctgggcctg ggcgtgacct cttggagcga gaacggccag aacatgggcg
     2281 tgtaccccgg cgtgttcgtg aacaagggcc tgaacagcaa cggcatcctg accttccagc
     2341 tgaccctggt gcccggcgtg cagctgcccc ccgacaacag cagactgcgg cagctggcct
     2401 tcttcgacca gagcgacgac tccatcctgg atttcttcat cgtgtccctg aacagccacg
     2461 accagcccag cgcccagctg ttcctgagca ccgtggatac cgacccctac ttcctgaaag
     2521 tgaccgtgct gaatggcctg tgcagcagcg ccgacagctg ctccgacggc aagctgcctt
     2581 acggcctgac cgtgcccggc atcagcagca acctgaatac cgaggccgct gccggcggaa
     2641 ggcatctgtc tgccgctctg ctgagcagcc agagctgctg cagtgccctg caggtcccct
     2701 tcgccatctt cggcctgggc cagttcgcca actacgtgga aaagctgacc atcagcatcc
     2761 cccccagcaa agagctgatc agaagccggc tgctgagctt catcgtgccc aaggcccaga
     2821 ttgtgatcaa cccctacccc ctggacaacc ctagcgcctg gaccatgaag ctgtttctgc
     2881 agcccctgta caacatgaag gtgctgtata tcgccatcac tggcgcgccg tcgacctcca
     2941 tcacggccta taagagtgag ggggagtcag cggagttctc cttcccactc aaccttggag
     3001 aggaaagcct gcagggagag ttgagatgga aggcagagaa ggctccttct tcccagtcct
     3061 ggatcacctt ctccctaaag aaccaaaagg tgtctgtgca gaagtctact agcaacccca
     3121 agttccagct gtccgaaacg ctcccactca cccttcagat accccaggtc tcccttcagt
     3181 ttgctggttc tggcaacctg accctgactc tggacagagg gatactgtat caggaagtga
     3241 acctggtggt gatgaaagtg actcagcccg acagcaacac tttgacctgt gaggtgatgg
     3301 gacccacctc acccaagatg agactgatct tgaagcagga gaatcaggag gccagggtct
     3361 ccaggcagga gaaagtgatt caagtgcagg cccctgaagc aggggtgtgg caatgtctac
     3421 tgagtgaagg tgaagaggtc aagatggact ccaagatcca ggttttatcc aaagggttga
     3481 attccggcag cctgcaccac atcctggacg cccagaagat gctgtggaac cacagggaca
     3541 ggaacctgcc tcctctggcc cctctgggcc cccaccacca tcaccaccat tgagtctaga
     3601 cacgtgatta attaaggatc ccccgacctc gacctctggc taataaagga aatttatttt
     3661 cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc
     3721 aaatcatttg gtcgagatcc ctcggagatc tctagctaga ggatcgatcc ccgccccgga
     3781 cgaactaaac ctgactacga catctctgcc ccttcttcgc ggggcagtgc atgtaatccc
     3841 ttcagttggt tggtacaact tgccaactgg gccctgttcc acatgtgaca cgggggggga
     3901 ccaaacacaa aggggttctc tgactgtagt tgacatcctt ataaatggat gtgcacattt
     3961 gccaacactg agtggctttc atcctggagc agactttgca gtctgtggac tgcaacacaa
     4021 cattgccttt atgtgtaact cttggctgaa gctcttacac caatgctggg ggacatgtac
     4081 ctcccagggg cccaggaaga ctacgggagg ctacaccaac gtcaatcaga ggggcctgtg
     4141 tagctaccga taagcggacc ctcaagaggg cattagcaat agtgtttata aggccccctt
     4201 gttaacccta aacgggtagc atatgcttcc cgggtagtag tatatactat ccagactaac
     4261 cctaattcaa tagcatatgt tacccaacgg gaagcatatg ctatcgaatt agggttagta
     4321 aaagggtcct aaggaacagc gatatctccc accccatgag ctgtcacggt tttatttaca
     4381 tggggtcagg attccacgag ggtagtgaac cattttagtc acaagggcag tggctgaaga
     4441 tcaaggagcg ggcagtgaac tctcctgaat cttcgcctgc ttcttcattc tccttcgttt
     4501 agctaataga ataactgctg agttgtgaac agtaaggtgt atgtgaggtg ctcgaaaaca
     4561 aggtttcagg tgacgccccc agaataaaat ttggacgggg ggttcagtgg tggcattgtg
     4621 ctatgacacc aatataaccc tcacaaaccc cttgggcaat aaatactagt gtaggaatga
     4681 aacattctga atatctttaa caatagaaat ccatggggtg gggacaagcc gtaaagactg
     4741 gatgtccatc tcacacgaat ttatggctat gggcaacaca taatcctagt gcaatatgat
     4801 actggggtta ttaagatgtg tcccaggcag ggaccaagac aggtgaacca tgttgttaca
     4861 ctctatttgt aacaagggga aagagagtgg acgccgacag cagcggactc cactggttgt
     4921 ctctaacacc cccgaaaatt aaacggggct ccacgccaat ggggcccata aacaaagaca
     4981 agtggccact cttttttttg aaattgtgga gtgggggcac gcgtcagccc ccacacgccg
     5041 ccctgcggtt ttggactgta aaataagggt gtaataactt ggctgattgt aaccccgcta
     5101 accactgcgg tcaaaccact tgcccacaaa accactaatg gcaccccggg gaatacctgc
     5161 ataagtaggt gggcgggcca agataggggc gcgattgctg cgatctggag gacaaattac
     5221 acacacttgc gcctgagcgc caagcacagg gttgttggtc ctcatattca cgaggtcgct
     5281 gagagcacgg tgggctaatg ttgccatggg tagcatatac tacccaaata tctggatagc
     5341 atatgctatc ctaatctata tctgggtagc ataggctatc ctaatctata tctgggtagc
     5401 atatgctatc ctaatctata tctgggtagt atatgctatc ctaatttata tctgggtagc
     5461 ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt
     5521 atatgctatc ctaatctgta tccgggtagc atatgctatc ctaatagaga ttagggtagt
     5581 atatgctatc ctaatttata tctgggtagc atatactacc caaatatctg gatagcatat
     5641 gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagcatag
     5701 gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagtatat
     5761 gctatcctaa tttatatctg ggtagcatag gctatcctaa tctatatctg ggtagcatat
     5821 gctatcctaa tctatatctg ggtagtatat gctatcctaa tctgtatccg ggtagcatat
     5881 gctatcctca tgataagctg tcaaacatga gaattttctt gaagacgaaa gggcctcgtg
     5941 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     6001 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     6061 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     6121 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     6181 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     6241 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     6301 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     6361 tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     6421 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     6481 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     6541 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     6601 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     6661 atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     6721 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     6781 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     6841 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     6901 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     6961 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     7021 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     7081 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     7141 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     7201 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     7261 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     7321 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     7381 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     7441 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     7501 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     7561 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     7621 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     7681 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     7741 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     7801 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     7861 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     7921 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     7981 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     8041 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     8101 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
     8161 ctctagctag aggtcgacca attctcatgt ttgacagctt atcatcgcag atccgggcaa
     8221 cgttgttgcc attgctgcag gcgcagaact ggtaggtatg gaagatctat acattgaatc
     8281 aatattggca attagccata ttagtcattg gttatatagc ataaatcaat attggctatt
     8341 ggccattgca tacgttgtat ctatatcata atatgtacat ttatattggc tcatgtccaa
     8401 tatgaccgcc atgtt
//
