LOCUS       sequence-234860-        2958 bp    DNA     circular SYN 15-APR-2025
DEFINITION  _-factor GPCR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Shaw WM, Yamauchi H, Mead J, Gowers GF, Bell DJ, Oling D, Larsson N,
            Wigglesworth M, Ladds G, Ellis T
  TITLE     Engineering a Model Cell for Rational Tuning of GPCR Signaling.
  JOURNAL   Cell. 2019 Apr 18;177(3):782-796.e27. doi: 
            10.1016/j.cell.2019.02.023. Epub 2019 Apr 4.
  PUBMED    30955892
REFERENCE   2  (bases 1 to 2958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2019.02.023"; journalName: "Cell"; date: 
            "2019-04-18- 18"; volume: "177"; issue: "3"; pages: "782-796.e27"
COMMENT     Sequence Label: pWS067
FEATURES             Location/Qualifiers
     source          1..2958
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1325..1359)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1430..2089)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2004..2023
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      2271..2859
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2760..2779
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agtcggtctc atatgtctga tgcggctcct tcattgagca atctatttta tgatccaacg
       61 tataatcctg gtcaaagcac cattaactac acttccatat atgggaatgg atctaccatc
      121 actttcgatg agttgcaagg tttagttaac agtactgtta ctcaggccat tatgtttggt
      181 gtcagatgtg gtgcagctgc tttgactttg attgtcatgt ggatgacatc gagaagcaga
      241 aaaacgccga ttttcattat caaccaagtt tcattgtttt taatcatttt gcattctgca
      301 ctctatttta aatatttact gtctaattac tcttcagtga cttacgctct caccggattt
      361 cctcagttca tcagtagagg tgacgttcat gtttatggtg ctacaaatat aattcaagtc
      421 cttcttgtgg cttctattga gacttcactg gtgtttcaga taaaagttat tttcacaggc
      481 gacaacttca aaaggatagg tttgatgctg acgtcgatat ctttcacttt agggattgct
      541 acagttacca tgtattttgt aagcgctgtt aaaggtatga ttgtgactta taatgatgtt
      601 agtgccaccc aagataaata cttcaatgca tccacaattt tacttgcatc ctcaataaac
      661 tttatgtcat ttgtcctggt agttaaattg attttagcta ttagatcaag aagattcctt
      721 ggtcttaagc agttcgatag tttccatatt ttactcataa tgtcatgtca atctttgttg
      781 gttccatcga taatattcat cctcgcatac agtttgaaac caaaccaggg aacagatgtc
      841 ttgactactg ttgcaacatt acttgctgta ttgtctttac cattatcatc aatgtgggcc
      901 acggctgcta ataatgcatc caaaacaaac acaattactt cagactttac aacatccaca
      961 gataggtttt atccaggcac gctgtctagc tttcaaactg atagtatcaa caacgatgct
     1021 aaaagcagtc tcagaagtag attatatgac ctatatccta gaaggaagga aacaacatcg
     1081 gataaacatt cggaaagaac ttttgtttct gagactgcag atgatataga gaaaaatcag
     1141 ttttatcagt tgcccacacc tacgagttca aaaaatacta ggataggacc gtttgctgat
     1201 gcaagttaca aagagggaga agttgaaccc gtcgacatgt acactcccga tacggcagct
     1261 gatgaggaag ccagaaagtt ctggactgaa gataataata atttaggatc ctgagaccag
     1321 accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat ggagttctga
     1381 ggtcattact ggatctatca acaggagtcc aagcgagctc gatatcaaat tacgccccgc
     1441 cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat
     1501 cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat
     1561 aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat
     1621 caaaactggt gaaactcacc cagggattgg ctgaaacgaa aaacatattc tcaataaacc
     1681 ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta
     1741 gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct
     1801 catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca
     1861 ttgccatacg aaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg
     1921 gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa
     1981 cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat
     2041 gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct
     2101 tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat
     2161 ggtgaaagtt ggaacctctt acgtgcccga tcaatcatga ccaaaatccc ttaacgtgag
     2221 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     2281 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     2341 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     2401 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     2461 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     2521 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     2581 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     2641 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     2701 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     2761 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     2821 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     2881 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     2941 gattctgtgg ataaccgt
//
