LOCUS       sequence-235012-        9575 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Bacterial expression of MBP-Pumillio2 fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9575)
  AUTHORS   Tichon A, Gil N, Lubelsky Y, Havkin Solomon T, Lemze D, Itzkovitz S,
            Stern-Ginossar N, Ulitsky I
  TITLE     A conserved abundant cytoplasmic long noncoding RNA modulates 
            repression by Pumilio proteins in human cells.
  JOURNAL   Nat Commun. 2016 Jul 13;7:12209. doi: 10.1038/ncomms12209.
  PUBMED    27406171
REFERENCE   2  (bases 1 to 9575)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-13"; volume: "7:12209. doi"; pages: " 
            10.1038/ncomms12209"
COMMENT     Sequence Label: pMAL-PUM2
FEATURES             Location/Qualifiers
     source          1..9575
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..80
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             81..1163
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(100..119)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        join(1406..1411,1412..1427,1428..1434)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    1442..1458
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1447..1469
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             1528..2628
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKTEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGSYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     regulatory      2485..2494
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     2602..2625
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             2641..2658
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             2680..2700
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(5668..5685)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5668..5684)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5677..5699)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5863..5880)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      6033..6119
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      6211..6238
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        6257..6348
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6349..6417,6418..7209)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6567..6586)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(7251..7764)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7446..7467)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7658..7677
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      7875..8463
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8364..8383
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8617..8634
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8649..8789
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8715..8734)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8875..8897
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(8891..9082)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 ccgacaccat cgaatggtgc aaaacctttc gcggtatggc atgatagcgc ccggaagaga
       61 gtcaattcag ggtggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg
      121 gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa
      181 cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac
      241 aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc
      301 acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg
      361 tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc
      421 ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca
      481 ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga
      541 cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc
      601 tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg
      661 cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag
      721 cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga
      781 atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa
      841 tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg
      901 acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc
      961 gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga
     1021 agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata
     1081 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     1141 cccgactgga aagcgggcag tgagcgcaac gcaattaatg taagttagct cactcattag
     1201 gcacaattct catgtttgac agcttatcat cgactgcacg gtgcaccaat gcttctggcg
     1261 tcaggcagcc atcggaagct gtggtatggc tgtgcaggtc gtaaatcact gcataattcg
     1321 tgtcgctcaa ggcgcactcc cgttctggat aatgtttttt gcgccgacat cataacggtt
     1381 ctggcaaata ttctgaaatg agctgttgac aattaatcat cggctcgtat aatgtgtgga
     1441 attgtgagcg gataacaatt tcacacagga aacagccagt ccgtttaggt gttttcacga
     1501 gcacttcacc aacaaggacc atagattatg aaaactgaag aaggtaaact ggtaatctgg
     1561 attaacggcg ataaaggcta taacggtctc gctgaagtcg gtaagaaatt cgagaaagat
     1621 accggaatta aagtcaccgt tgagcatccg gataaactgg aagagaaatt cccacaggtt
     1681 gcggcaactg gcgatggccc tgacattatc ttctgggcac acgaccgctt tggtggctac
     1741 gctcaatctg gcctgttggc tgaaatcacc ccggacaaag cgttccagga caagctgtat
     1801 ccgtttacct gggatgccgt acgttacaac ggcaagctga ttgcttaccc gatcgctgtt
     1861 gaagcgttat cgctgattta taacaaagat ctgctgccga acccgccaaa aacctgggaa
     1921 gagatcccgg cgctggataa agaactgaaa gcgaaaggta agagcgcgct gatgttcaac
     1981 ctgcaagaac cgtacttcac ctggccgctg attgctgctg acgggagtta tgcgttcaag
     2041 tatgaaaacg gcaagtacga cattaaagac gtgggcgtgg ataacgctgg cgcgaaagcg
     2101 ggtctgacct tcctggttga cctgattaaa aacaaacaca tgaatgcaga caccgattac
     2161 tccatcgcag aagctgcctt taataaaggc gaaacagcga tgaccatcaa cggcccgtgg
     2221 gcatggtcca acatcgacac cagcaaagtg aattatggtg taacggtact gccgaccttc
     2281 aagggtcaac catccaaacc gttcgttggc gtgctgagcg caggtattaa cgccgccagt
     2341 ccgaacaaag agctggcaaa agagttcctc gaaaactatc tgctgactga tgaaggtctg
     2401 gaagcggtta ataaagacaa accgctgggt gccgtagcgc tgaagtctta cgaggaagag
     2461 ttggcgaaag atccacgtat tgccgccacc atggaaaacg cccagaaagg tgaaatcatg
     2521 ccgaacatcc cgcagatgtc cgctttctgg tatgccgtgc gtactgcggt gatcaacgcc
     2581 gccagcggtc gtcagactgt cgatgaagcc ctgaaagacg cgcagactaa ttcgagctca
     2641 catcatcatc atcatcatga ttatgatatc ccaaccaccg aaaatttgta ttttcaaggg
     2701 atgaatcatg attttcaagc tcttgcatta gaatctcggg gaatgggaga gcttttgcct
     2761 accaaaaagt tttgggaacc tgatgattca acaaaagatg gacaaaaagg catatttctt
     2821 ggggatgatg aatggagaga gactgcatgg ggagcttctc accattcaat gtcccagcct
     2881 attatggtac agagaagatc tggacagggt tttcatggaa acagtgaagt aaatgcaata
     2941 ctgtctccgc gatcagaaag tggaggcctt ggtgtgagca tggtagaata tgtattaagt
     3001 tcttctcctg ctgataaatt ggattctcga tttaggaagg gaaattttgg cactagagat
     3061 gctgaaacag atggacctga gaaaggagat caaaaaggca aggcttctcc atttgaggag
     3121 gaccaaaaca gagatcttaa acaaggagat gatgatgatt ctaaaataaa tggcagaggt
     3181 ttgccaaatg gaatggatgc cgattgcaaa gattttaatc gtactcctgg aagtcgtcaa
     3241 gcctctccaa ctgaagtagt tgagcgcttg ggccccaata ctaatccctc agaaggactg
     3301 gggcctcttc ctaatcctac agctaataaa ccacttgttg aagaattttc aaatcctgaa
     3361 actcagaatc tggatgccat ggaacaagtt ggtctggaat ccttacagtt tgactatcct
     3421 ggtaatcagg taccaatgga ctcttcagga gctactgtag gcctttttga ctacaattcc
     3481 cagcagcagc tctttcagag gactaatgca ctaacagttc aacagttaac tgcagctcaa
     3541 cagcagcaat atgcattagc agcagctcag cagccacata tagctggtgt attctcagca
     3601 ggccttgctc cagctgcatt tgtgccaaat ccatacatta ttagtgctgc tcctccaggg
     3661 accgatccgt atactgcagc aggattggct gcagcagcta cattagcagg tccagcagtg
     3721 gttccacctc agtattacgg cgttccatgg ggggtgtatc cagccaactt atttcagcag
     3781 caagctgcag ctgcggcaaa taacacagcc agtcagcaag cagcatcaca agctcagcct
     3841 ggacagcaac aggttctccg tgctggagca ggtcagcgtc ctcttactcc caatcagggt
     3901 cagcaagggc agcaagcaga atcacttgcg gcagctgcag cagcaaatcc aacattggct
     3961 tttggtcagg gtcttgctac tggcatgcca ggctatcaag tactagctcc aactgcctat
     4021 tatgatcaga ctggtgcctt agtggttggc cctggagcaa ggactggcct tggagctcca
     4081 gttcggttaa tggctccaac acctgtttta attagttcag cagcagcaca agctgcagca
     4141 gcagcagcag ctggaggaac tgcaagtagc cttacaggca gcacaaatgg tctgtttcgg
     4201 ccaattggca ctcagccacc acagcagcag caacagcagc caagcactaa tctgcaatct
     4261 aattcatttt atggaagcag ttctttgact aatagctccc agagtagttc tttattttct
     4321 catggacctg gtcaacctgg aagtacatct cttggctttg gaagtggtaa ctctttgggt
     4381 gctgctatag gctcagccct cagtggattt ggttcatcag ttggcaggac aaatggtagt
     4441 ggtcgatata tctctgcagc acctggagca gaagcaaaat atcgaagtgc ttcaagcact
     4501 tccagtctat ttagctccag cagccagctc tttcctcctt cccggcttcg gtataatagg
     4561 tctgatatta tgccttctgg ccgcagtaga ttattggaag atttcagaaa caaccgcttc
     4621 ccaaaccttc agcttagaga cttgattgga catatagttg agttttctca agaccagcat
     4681 ggttctagat tcatacagca aaaactagag agagctactc cagctgagcg acagatggta
     4741 tttaatgaaa ttctgcaagc agcctatcaa ttaatgactg atgtttttgg caactatgtt
     4801 atacagaagt tttttgagtt tgggagtctg gatcaaaaat tagccctggc tactcgtatt
     4861 cgtggtcatg ttctaccctt agccttgcag atgtatggct gccgcgttat tcagaaagca
     4921 ttagaatcta tttcttctga ccagcagagt gaaatggtaa aggagctgga tggtcatgtg
     4981 ctcaaatgtg tgaaagatca gaatggaaac catgttgtac aaaaatgtat cgaatgtgtt
     5041 cagccacagt cactacagtt catcattgat gctttcaagg gacaagtatt tgtgctttca
     5101 actcatcctt atggctgcag agtaattcag cgcatcctag agcattgcac tgcagaacag
     5161 accttaccta tcttagaaga actccaccaa catacagagc agttggtaca ggatcagtat
     5221 ggcaattatg ttattcagca tgtactggaa cacggtcgac ctgaagacaa gagcaaaatt
     5281 gtttccgaaa tcaggggaaa ggttttagcc ctgagtcaac acaaatttgc cagcaatgta
     5341 gtagaaaagt gtgttactca tgcctcccgt gctgagagag ctttactgat tgacgaggtt
     5401 tgctgccaga atgatggtcc tcacagtgcc ttatacacca tgatgaagga ccagtatgcc
     5461 aattacgtgg ttcaaaagat gattgatatg gctgaacctg ctcagagaaa gataatcatg
     5521 cacaagattc gacctcacat tactactttg cgcaaataca catacgggaa gcatatactg
     5581 gccaagttgg aaaagtatta tttgaagaat agcccggacc taggacctat tggaggacca
     5641 ccaaatggaa tgctgtaaaa gcttggcact ggccgtcgtt ttacaacgtc gtgactggga
     5701 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     5761 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     5821 atggcagctt ggctgttttg gcggatgaga taagattttc agcctgatac agattaaatc
     5881 agaacgcaga agcggtctga taaaacagaa tttgcctggc ggcagtagcg cggtggtccc
     5941 acctgacccc atgccgaact cagaagtgaa acgccgtagc gccgatggta gtgtggggtc
     6001 tccccatgcg agagtaggga actgccaggc atcaaataaa acgaaaggct cagtcgaaag
     6061 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc
     6121 cgccgggagc ggatttgaac gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc
     6181 cgccataaac tgccaggcat caaattaagc agaaggccat cctgacggat ggcctttttg
     6241 cgtttctaca aactcttttg tttatttttc taaatacatt caaatatgta tccgctcatg
     6301 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     6361 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     6421 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     6481 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttc
     6541 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc
     6601 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     6661 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     6721 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     6781 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     6841 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     6901 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     6961 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     7021 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     7081 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     7141 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     7201 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt accccggttg
     7261 ataatcagaa aagccccaaa aacaggaaga ttgtataagc aaatatttaa attgtaaacg
     7321 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
     7381 aggccgaaat cggcaaaatc ccttataaat caaaagaata gcccgagata gggttgagtg
     7441 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
     7501 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcacccaaa tcaagttttt
     7561 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
     7621 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
     7681 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
     7741 ttaatgcgcc gctacagggc gcgtaaaagg atctaggtga agatcctttt tgataatctc
     7801 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     7861 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     7921 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     7981 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     8041 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     8101 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     8161 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     8221 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     8281 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cgaaacagga
     8341 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     8401 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     8461 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     8521 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     8581 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     8641 gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     8701 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc
     8761 tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc
     8821 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     8881 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa
     8941 gctcatcagc gtggtcgtgc agcgattcac agatgtctgc ctgttcatcc gcgtccagct
     9001 cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg
     9061 cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg
     9121 taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg
     9181 cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga
     9241 gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg
     9301 gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc
     9361 gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg
     9421 cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct
     9481 aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc
     9541 gcacccgtgg ccaggaccca acgctgcccg aaatt
//
