LOCUS       sequence-235297-        9687 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expresses GFP tagged BioID and human FAM21C in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9687)
  AUTHORS   Hoyer MJ, Chitwood PJ, Ebmeier CC, Striepen JF, Qi RZ, Old WM, 
            Voeltz GK
  TITLE     A Novel Class of ER Membrane Proteins Regulates ER-Associated 
            Endosome Fission.
  JOURNAL   Cell. 2018 Sep 20;175(1):254-265.e14. doi: 
            10.1016/j.cell.2018.08.030. Epub 2018 Sep 13.
  PUBMED    30220460
REFERENCE   2  (bases 1 to 9687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.08.030"; journalName: "Cell"; date: 
            "2018-09-20- 20"; volume: "175"; issue: "1"; pages: "254-265.e14"
COMMENT     Sequence Label: GFP-BioID-FAM21
FEATURES             Location/Qualifiers
     source          1..9687
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      243..252
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             249..965
                     /codon_start=1
                     /product="Aequorea coerulescens GFP"
                     /label=AcGFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMDELYK"
     CDS             join(987..1334,1335..1337,1338..1946)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             complement(3313..3336)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     protein_bind    5568..5579
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     polyA_signal    6111..6232
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6148..6167)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6202..6221
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(6239..6694)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6376..6397)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6588..6607
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6721..6825
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6827..7184
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6827..7018
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6850..6870)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7035..7170
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7219..8013
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7219..8013
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7273..7292)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7883..7902
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8201..8220)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8245..8292
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      8621..9209
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9110..9129
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        9384..9687
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gcgctaccgg
      241 tcgccaccat ggtgagcaag ggcgccgagc tgttcaccgg catcgtgccc atcctgatcg
      301 agctgaatgg cgatgtgaat ggccacaagt tcagcgtgag cggcgagggc gagggcgatg
      361 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cctgtgccct
      421 ggcccaccct ggtgaccacc ctgagctacg gcgtgcagtg cttctcacgc taccccgatc
      481 acatgaagca gcacgacttc ttcaagagcg ccatgcctga gggctacatc caggagcgca
      541 ccatcttctt cgaggatgac ggcaactaca agtcgcgcgc cgaggtgaag ttcgagggcg
      601 ataccctggt gaatcgcatc gagctgaccg gcaccgattt caaggaggat ggcaacatcc
      661 tgggcaataa gatggagtac aactacaacg cccacaatgt gtacatcatg accgacaagg
      721 ccaagaatgg catcaaggtg aacttcaaga tccgccacaa catcgaggat ggcagcgtgc
      781 agctggccga ccactaccag cagaataccc ccatcggcga tggccctgtg ctgctgcccg
      841 ataaccacta cctgtccacc cagagcgccc tgtccaagga ccccaacgag aagcgcgatc
      901 acatgatcta cttcggcttc gtgaccgccg ccgccatcac ccacggcatg gatgagctgt
      961 acaagtccgg actcagatct cgagctaagg acaacaccgt gcccctgaag ctgatcgccc
     1021 tgctggccaa cggcgagttc cactctggcg agcagctggg agagaccctg ggaatgagca
     1081 gagccgccat caacaagcac atccagacac tgagagactg gggagtggac gtgttcaccg
     1141 tgcctggcaa gggctacagc ctgcctgagc ctatccagct gctgaacgcc aagcagatcc
     1201 tgggacagct ggatggcgga agcgtggccg tgctgcctgt gatcgactcc accaatcagt
     1261 acctgctgga cagaatcgga gagctgaagt ccggcgacgc ctgcatcgcc gagtaccagc
     1321 aggctggcag aggaggcaga ggacggaagt ggttcagccc attcggagcc aacctgtacc
     1381 tgtccatgtt ctggagactg gagcagggac ctgctgctgc catcggactg agtctggtga
     1441 tcggaatcgt gatggccgag gtgctgagaa agctgggagc cgacaaggtg agagtgaagt
     1501 ggcctaatga cctgtacctc caggaccgca agctggctgg catcctggtg gagctgacag
     1561 gcaagacagg cgatgccgct cagatcgtga tcggagccgg aatcaacatg gccatgagaa
     1621 gagtggagga gagcgtggtg aaccagggct ggatcaccct gcaggaggct ggcatcaacc
     1681 tggaccggaa caccctggcc gccatgctga tcagagagct gagagccgct ctggagctgt
     1741 tcgagcagga gggactggct ccttacctga gcagatggga gaagctggac aacttcatca
     1801 acagacctgt gaagctgatc atcggcgaca aggaaatctt cggcatctcc agaggaatcg
     1861 acaagcaggg agctctgctg ctggagcagg acggaatcat caagccctgg atgggcggag
     1921 aaatctccct gagaagcgca gagaaggagc tcggtaccat gatgaaccgg acgacccccg
     1981 accaggagct ggtgccggcg tcggagcccg tgtgggagcg gccgtggtcg gtggaggaga
     2041 tccgcaggag cagccagagc tggtcgctgg cggccgacgc gggcctacta cagtttctac
     2101 aggaattctc acagcaaact atctctagga cccatgaaat caagaaacaa gtggacggac
     2161 taatccggga aaccaaagcc acagattgtc gcctgcataa tgtcttcaat gacttcctta
     2221 tgctctctaa tacccagttc atagagaatc gtgtatatga tgaagaagtg gaggagccag
     2281 tactcaaggc tgaggcagaa aaaacagagc aggagaagac acgagagcag aaagaagtag
     2341 atctcattcc taaagtccag gaggctgtga actatggctt acaagtattg gacagtgcct
     2401 ttgagcaact tgatatcaaa gcaggaaact cagactccga ggaagatgat gctaatgggc
     2461 gggtggaact gatccttgaa ccaaaggatc tatacattga tcgtccttta ccatatctca
     2521 ttgggtcaaa gctgttcatg gaacaagaag atgtaggtct tggagagctg tccagtgaag
     2581 aaggctctgt gggcagtgat cgtggcagta ttgtggacac tgaggaagag aaagaagagg
     2641 aggagtcaga tgaagatttt gcccatcaca gtgacaatga acaaaaccag cacaccacac
     2701 aaatgagtga tgaggaagag gatgatgatg gctgtgacct ttttgctgac tctgagaagg
     2761 aggaggaaga tattgaggac attgaagaaa atactagacc taaaagaagc agacctacat
     2821 cgtttgcaga tgagctggct gcccgcatca agggggatgc catgggtcga gtggacgagg
     2881 agccgacaac cttaccctca ggagaagcaa aacctcggaa gacactcaaa gagaagaagg
     2941 aaaggagaac tccttcagac gatgaagagg ataacttatt cgcacccccc aagctgaccg
     3001 acgaggactt ctcgccattt ggctctggag gtggcctgtt cagtggcggc aaggggctat
     3061 ttgatgatga ggacgaggag agtgacctct tcacggaagc ctcccaggat cggcaagctg
     3121 gagcctctgt taaggaggag tcttcatcat ccaaacctgg aaagaagatc ccagcaggag
     3181 ctgtttctgt atttttagga gacacggatg tgtttggtgc tgcctccgtt ccatcactga
     3241 aggagccaca gaagcctgag cagcccactc caaggaaaag cccctacggt ccccctccca
     3301 ctggcctctt tgatgatgat gatggtgatg atgatgacga ctttttctcg gcaccccaca
     3361 gcaaaccttc taaaacacgc aaagtccaat ccactgccga tatctttggt gacgaagaag
     3421 gagatctgtt caaagaaaaa gccgtagcat cgccagaagc cactgtgagt cagacagatg
     3481 aaaataaagc aagagcagaa aaaaaggtta ccttatctta cagcaaaaat ctcaagccct
     3541 catcagaaac aaagactcaa aaaggcttat tttcagatga ggaggactct gaggatttgt
     3601 tttcttctca aagtgcgagt aacttaaaag gtgcatctct gctgcctggc aagctcccca
     3661 cgtcggtttc cctgtttgat gatgaagatg aagaggataa tctttttggg ggtacagctg
     3721 ctaagaagca gacattgtct ctacaagctc agagagaaga gaaagcaaaa gcctccgagc
     3781 tctccaaaaa gaaagcatct gccctgttgt tcagcagtga tgaggaggac cagtggaata
     3841 ttcctgcttc acagacccac ttagcatctg acagcaggtc taaaggagaa cccagggatt
     3901 ctgggaccct ccagagccag gaggccaagg ctgtgaaaaa gaccagtctc tttgaggaag
     3961 acaaagaaga tgatcttttt gccattgcca aggacagcca aaagaagacc cagagagtgt
     4021 cactcctctt tgaagacgat gttgatagcg gaggctctct gtttggctct cctcccacat
     4081 ctgttcctcc tgcaacaaag aaaaaagaga ctgtctctga ggcaccacct ttgctgttca
     4141 gcgatgaaga agagaaggag gcacaacttg gagtgaagtc tgtggataag aaggttgaga
     4201 gtgccaagga gtcattaaaa tttgggagaa ctgatgtggc tgagtcagaa aaggaaggac
     4261 ttttgactag atctgctcag gaaacagtca agcattctga tttattttct tcatcatccc
     4321 catgggacaa aggaaccaag cctagaacca aaactgttct tagcttgttt gatgaggaag
     4381 aggataaaat ggaagatcaa aacattatcc aggctccaca gaaagaagta ggaaagggct
     4441 gcgatcctga tgcccacccc aagagcacag gtgtcttcca ggatgaagag ctgcttttca
     4501 gccacaagct ccaaaaggac aatgacccag atgttgacct ttttgctggc accaaaaaaa
     4561 ccaagctgtt agagccaagt gttgggagcc tgtttgggga tgatgaagat gatgatcttt
     4621 tcagctctgc caagtcccag cctttggtac aagagaaaaa gagagtagtg aaaaaagacc
     4681 actctgttaa ctctttcaaa aaccagaaac atcctgaatc cattcaaggt agtaaagaaa
     4741 aaggcatatg gaagccggaa acacctcagg actcatcagg tctcgctcca tttaaaacca
     4801 aagaaccatc cactcggatc gggaagatac aagcaaattt agcgatcaac ccagcggcct
     4861 tgctgcccac agcggcttcc cagatctctg aagtaaagcc tgttttgcca gaattggctt
     4921 ttccttcatc tgaacacaga aggagccacg gtctagaaag tgtgcctgtc cttcccggga
     4981 gtggggaggc cggtgtgagt tttgatcttc cagctcaggc agacacctta cacagtgcaa
     5041 acaagagccg tgtcaagatg agagggaagc gtagaccgca gacccgtgca gctaggcggc
     5101 tggctgctca ggagtccagc gaggctgagg acatgagcgt ccccagagga cccattgcac
     5161 agtgggctga tggcgccatt tccccaaatg gccatcggcc acagctcaga gcagccagtg
     5221 gagaagacag cactgaggag gccctggcag ctgccgctgc accttgggaa ggtggtcctg
     5281 tgcctggagt ggacacaagc ccctttgcaa agtctctggg tcattccaga ggggaggctg
     5341 acctttttga ttctggggac attttttcca cgggcactgg atctcagtcc gtggagagaa
     5401 caaaacccaa ggcaaagata gcagagaatc ctgccaaccc accagtgggt ggtaaagcaa
     5461 agagccccat gtttcctgct ctaggcgagg ccagcagtga tgatgatctc tttcagtctg
     5521 ctaaaccaaa accagcaaag aaaacaaatc cctttcctct cctggaagat gaggatgacc
     5581 tctttacaga tcagaaagtc aagaagaatg agacaaaatc cagtagtcag caggatgtca
     5641 tattaacaac acaagatatt tttgaggatg atatatttgc tacggaagca attaaaccct
     5701 ctcagaaaac cagagagaag gagaaaacat tggaatctaa tttatttgat gataacattg
     5761 atatctttgc tgacttaact gtaaaaccaa aagaaaagtc caaaaagaaa gtggaagcca
     5821 agtctatatt tgatgatgat atggatgaca tcttctccac tggtatccag gctaagacaa
     5881 ccaaaccaaa aagccgatct gcacaggccg cacctgaacc aagatttgaa cacaaggtgt
     5941 ccaacatctt tgatgatccc ctgaatgcct ttggaggcca gggatccacc ggatctagat
     6001 aactgatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
     6061 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
     6121 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     6181 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct taacgcgtaa
     6241 attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt
     6301 tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata
     6361 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac
     6421 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa
     6481 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc
     6541 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
     6601 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
     6661 cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg
     6721 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     6781 caataaccct gataaatgct tcaataatat tgaaaaagga agagtcctga ggcggaaaga
     6841 accagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     6901 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     6961 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     7021 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     7081 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     7141 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaagatcga tcaagagaca
     7201 ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct
     7261 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
     7321 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
     7381 ggtgccctga atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc
     7441 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
     7501 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
     7561 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
     7621 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
     7681 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
     7741 aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg
     7801 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
     7861 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
     7921 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
     7981 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg
     8041 accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa
     8101 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
     8161 tcatgctgga gttcttcgcc caccctaggg ggaggctaac tgaaacacgg aaggagacaa
     8221 taccggaagg aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacggtgttg
     8281 ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac
     8341 cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa
     8401 gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcct
     8461 caggttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     8521 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     8581 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     8641 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     8701 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     8761 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     8821 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     8881 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     8941 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     9001 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     9061 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     9121 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     9181 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     9241 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     9301 ataaccgtat taccgccatg cattagttat taatagtaat caattacggg gtcattagtt
     9361 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     9421 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     9481 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     9541 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     9601 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     9661 tacgtattag tcatcgctat taccatg
//
