LOCUS       sequence-235412-        2441 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Promoter pTEF of Y. lipolytica for GoldenMOCS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Egermeier M, Sauer M, Marx H
  TITLE     Golden Gate based metabolic engineering strategy for wild-type 
            strains of Yarrowia lipolytica.
  JOURNAL   FEMS Microbiol Lett. 2019 Jan 29. pii: 5304171. doi: 
            10.1093/femsle/fnz022.
  PUBMED    30698703
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS 
            Microbiol Lett. 2019 Jan 29. pii: 5304171. doi: 
            10.1093/femsle/fnz022."
COMMENT     Sequence Label: BB1_12_YlpTEF
FEATURES             Location/Qualifiers
     source          1..2441
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     5..22
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6..22
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(742..758)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(742..758)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      806..837
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        838..940
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             941..1756
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCLKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1014..1033)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      1780..1807
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1819..2406)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1899..1918)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cccgtgtaaa acgacggcca gtttatctag tcagcttgat tctagctgat cgtggaccgg
       61 aaggtgagcc agtgagttga ttgcagtcca gttacgctgg agtctgaggc tcgtcctgaa
      121 tgatatgcga ccgccggagg gttgcgtttg agacgggcga cagatcgaca ctgctcgatc
      181 cgctcgcacc gaagacgcgg agagacaccg ggttggcggc gcatttgtgt cccaaaaaac
      241 agccccaatt gccccaattg accccaaatt gacccagtag cgggcccaac cccggcgaga
      301 gcccccttct ccccacatat caaacctccc ccggttccca cacttgccgt taagggcgta
      361 gggtactgca gtctggaatc tacgcttgtt cagactttgt tctagtttct ttgtctggcc
      421 atccgggtaa cccatgccgg acgcaaaata gactactgaa aatttttttg ctttgtggtt
      481 gggactttag ccaagggtat aaaagaccac cgtccccgaa ttacctttcc tcttcttttc
      541 tctctctcct tgtcaactca cacccgaaat cgttaagcat ttccttctga gtataagaat
      601 cattcaacat ggcgtcttcg gatcgacgag agcagcgcga ctggatcact actggaccgc
      661 gagctgtgct gcgacccgtg atcttacggc attatacgta tgatcggtcc acgatcagct
      721 agattatcta gtcagcttga tgtcatagct gtttcctgag gctcaatact gaccatttaa
      781 atcatacctg acctccatag cagaaagtca aaagcctccg accggaggct tttgacttga
      841 tcggcacgta agaggttcca actttcacca taatgaaata agatcactac cgggcgtatt
      901 ttttgagtta tcgagatttt caggagctaa ggaagctaaa atgagccata ttcaacggga
      961 aacgtcttgc ttgaagccgc gattaaattc caacatggat gctgatttat atgggtataa
     1021 atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgattgt atgggaagcc
     1081 cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga
     1141 tgagatggtc aggctaaact ggctgacgga atttatgcct cttccgacca tcaagcattt
     1201 tatccgtact cctgatgatg catggttact caccactgcg atcccaggga aaacagcatt
     1261 ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt
     1321 cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacggcg atcgcgtatt
     1381 tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttggtgcga gtgattttga
     1441 tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata aactcttgcc
     1501 attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga
     1561 cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca
     1621 ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct
     1681 ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc acttgatgct
     1741 cgatgagttt ttctaatgag gacctaaatg taatcacctg gctcaccttc gggtgggcct
     1801 ttctgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     1861 atgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     1921 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     1981 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     2041 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     2101 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     2161 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     2221 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     2281 tctgctgaag ccagttacct cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     2341 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     2401 tctcaagaag atcctttgat tttctaccga agaaaggccc a
//
