LOCUS       sequence-235945-        2159 bp    DNA     circular SYN 15-APR-2025
DEFINITION  MoClo golden gate assembly AB part for J23151 (strength ~900x 
            Anderson promoter; see 
            http://parts.igem.org/Promoters/Catalog/Anderson) - Please see 
            Supplemental Documents for annotated Genbank file.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2159)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p4m
FEATURES             Location/Qualifiers
     source          1..2159
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(147..938,939..1007))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     770..789
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1008..1112)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1180..1198
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1202..1245)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    1248..1269
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    1350..1370
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(1376..1393)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1547..2135)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1627..1646)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
       61 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
      121 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
      181 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
      241 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgcga cccacgctca
      301 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
      361 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
      421 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
      481 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
      541 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
      601 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
      661 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
      721 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
      781 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
      841 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
      901 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
      961 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     1021 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     1081 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     1141 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggcag
     1201 aatttcagat aaaaaaaatc cttagctttc gctaaggatg atttctggaa ttcgcggccg
     1261 cttctagaga ctagtgggtc tcaggagtgc tgttccgctg ggcatgcttg atggctagct
     1321 cagtcctagg tacaatgcta ctagagacct actagtagcg gccgctgcag gcttcctcgc
     1381 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     1441 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     1501 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cacaggctcc
     1561 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     1621 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     1681 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     1741 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     1801 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     1861 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     1921 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     1981 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     2041 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     2101 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacg
//
