LOCUS       sequence-235960-        5723 bp    DNA     circular SYN 15-APR-2025
DEFINITION  for generating kank3 riboprobes for WISH in zebrafish.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5723)
  AUTHORS   Hensley MR, Cui Z, Chua RF, Simpson S, Shammas NL, Yang JY, Leung 
            YF, Zhang G
  TITLE     Evolutionary and developmental analysis reveals KANK genes were 
            co-opted for vertebrate vascular development.
  JOURNAL   Sci Rep. 2016 Jun 13;6:27816. doi: 10.1038/srep27816.
  PUBMED    27292017
REFERENCE   2  (bases 1 to 5723)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-06-13"; volume: "6:27816. doi"; pages: " 
            10.1038/srep27816"
COMMENT     Sequence Label: kank3_pJet1.2
FEATURES             Location/Qualifiers
     source          1..5723
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(3180..3186,3187..3204,3205..3210))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    3225..3246
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3366..3383)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3537..4125)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3617..3636)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4296..5087,5088..5156))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4919..4938
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5157..5261)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5329..5347
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     5653..5672
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5653..5671
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 atgacccaat ctgtgcacat aacctccaaa gtgccaaatc tgagtgcccc ttttcattgc
       61 tctgctgagg aagaatcaga ccaaagtggt gcttattctg tacagacacc ctacggattc
      121 gagctggacc ttgatttcct caagtatgtt gaggaaatag agagtgggca cagcattcgt
      181 agggctaatg tgcaccctcg gagaggatct aggagtgaca ggcgaaattt gagtactggg
      241 ggtcgaacca gcggctggac ctccaccgag tccctttcct caacaactag tgagaatggt
      301 cagactaacc aaccttttct tcagagccca atcacaccac cttataaatc acaaccactt
      361 tccccattac cagtgatcag tcccagttta cctactttct caaaagtgcc accaccacct
      421 ccaccacgta accctagggt ggaaagaaca cttcttgaaa ctagcctccg gctccaacaa
      481 gagcaaagtc agttttccaa tggattaggc tccaatttgg ctgacctttc caaagtgagt
      541 tccaaaacca atgtatctaa tagggacttc tctgtaggtt cttcacagcc atcagaagcc
      601 ccattgctac cgatgtcaca tgttatgcag aacccccttt ctggggcatg gaccagagca
      661 agtcctcaga cttccggatg gagcactcca gcctcaaact caggaacaat gcctcctggg
      721 cagcttcaga cagttcgaga acagatggct gctgccctta gacagctgag agagatggaa
      781 gaaagagtaa aaggagtacc agtcctggaa agagaagttg ccatgctccg tgcagaaaaa
      841 gaaaggctat ctgtggagct tcaaaataaa acaaaggaac ttgatgcagc aatcaaactg
      901 caagtaccca ctgaggcagt agaggaacaa gacaaagagc aaatcaagaa aataccaacc
      961 tttgttttag aaacaaaatc catagcagta ggtaatgatg tacctcttga gaatgtgata
     1021 gtctctaatc gtcaaacgct caaggatgct gccgttggat caatcatcga tgtttgtcac
     1081 gcagctgttg aaactgaggc taatgccatg tgtgatgagg gaattcagac cacggtggat
     1141 acagaagatg tgtcagtctg ggtcattgag tcatttctag ggctacaaag cgaggcggaa
     1201 cgagaaattg acaacctaca acacaccatc acgtttcaac aggagtcaat tcaggttctc
     1261 gagacacgat taactcaggt caaccaagat ctcgaaactt taagggctct ggaaattgag
     1321 agaacctcca agatcatgtt ggacaaagag acgacagcta aaccagagac agtcagcgtc
     1381 caactggaaa catgtcctga agtttgttca gtgggagtgt tgtttcctga tgtgactgac
     1441 cctgagtatg atttaaaaaa gaaccaaagt attcaaacag atcctgagga aacttctaag
     1501 gaacaacctg tggagctaat aagcactggt gtccagtggg aatgtcttga tgacactgag
     1561 ccacaagaca gcgaggaaca gacaacgtct gctgagactg caggccctct gaaatctatc
     1621 atgaagagga aggatgggag tggtgctggt gcagcttcca gcagcggcaa aaaaagctta
     1681 caatttgtcg ggattctaaa tggagggtat gagtcgacgt ctagtgaaga ggaagaggag
     1741 gaggaagaga gttcctctga tggcagcgag gctggtgctt gttcagacag cagtgtggaa
     1801 gaagcagcag ctctagagga cacgtctgat gaagagatga atataaatgc ggatgaaagt
     1861 gatagcgatg aaaacatgcc agcagggact gaagagttaa aggatcaggc agaggaagtg
     1921 aaggaaaagt ttgaacttag ctccaaaatg cgtgaggctt gccttatttt gaagaaccat
     1981 cttaacgacg gagaaaaggc tgtaaagagt aaagaagtgt tgtccagcac tcatagcgtt
     2041 cagctggagt ggtttcgagt atccagtgca aagatggccc agccattacg tgtgtcaaat
     2101 tacctgatgg cgttctccga ggtctccccc gtcctgctgg agcacattgt caacatgacc
     2161 gacggcaacg gcaacactgc tttacactac agcgtctccc attccaactt cgctgttgtg
     2221 gacctactgc ttgacacagg catgtgcaat gttaatcagc agaataaagc cggatataca
     2281 gctgtgatgc tggccgctct ttcagcagtg aaggaggagg atgaaatggc ggtggtccag
     2341 aaactcttcc gtctgggaaa cgtcaatgcc aaagccagcc aagcaggaca gaccgctttg
     2401 atgctggccg tcagtcatgg gcgtcaggag atggtgcgag cgctgctaga ttgtggcgct
     2461 aatgtgaaca tccaagacga tgagggctcc accgctctaa tgtgcgcaag cgaacacggt
     2521 cgagctgaga tcgtctcctt actgctggaa cagccggggt gtgacatttc aatcgtcgac
     2581 aatgatggca gtaatgctct gtccattgct ctggaagcat cccacaatga catcgctgtt
     2641 ttactgtacg cccacatgaa ctactcaaaa acacaggcgg atgcatcacc taaagcaaac
     2701 tcccggagcc catccagccc tagaaaaaca tggccctcgg agaaaatctt tctagaagat
     2761 ctcctacaat attctcagct gccatggaaa atcgatgttc ttcttttatt ctctcaagat
     2821 tttcaggctg tatattaaaa cttatattaa gaactatgct aaccacctca tcaggaaccg
     2881 ttgtaggtgg cgtgggtttt cttggcaatc gactctcatg aaaactacga gctaaatatt
     2941 caatatgttc ctcttgacca actttattct gcattttttt tgaacgaggt ttagagcaag
     3001 cttcaggaaa ctgagacagg aattttatta aaaatttaaa ttttgaagaa agttcagggt
     3061 taatagcatc cattttttgc tttgcaagtt cctcagcatt cttaacaaaa gacgtctctt
     3121 ttgacatgtt taaagtttaa acctcctgtg tgaaattatt atccgctcat aattccacac
     3181 attatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     3241 acattaattg cgttgcgctc actgccaatt gctttccagt cgggaaacct gtcgtgccag
     3301 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     3361 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     3421 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     3481 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     3541 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     3601 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     3661 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     3721 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     3781 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     3841 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     3901 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     3961 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     4021 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     4081 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     4141 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     4201 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     4261 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     4321 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     4381 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     4441 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     4501 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     4561 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     4621 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     4681 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     4741 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     4801 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     4861 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     4921 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     4981 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     5041 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     5101 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     5161 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     5221 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     5281 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     5341 acgaggccgc ccctgcagcc gaattatatt atttttgcca aataattttt aacaaaagct
     5401 ctgaagtctt cttcatttaa attcttagat gatacttcat ctggaaaatt gtcccaatta
     5461 gtagcatcac gctgtgagta agttctaaac cattttttta ttgttgtatt atctctaatc
     5521 ttactactcg atgagttttc ggtattatct ctatttttaa cttggagcag gttccattca
     5581 ttgttttttt catcatagtg aataaaatca actgctttaa cacttgtgcc tgaacaccat
     5641 atccatccgg cgtaatacga ctcactatag ggagagcggc cgccagatct tccggatggc
     5701 tcgagttttt cagcaagatc acc
//
