LOCUS       sequence-236041-        2836 bp    DNA     circular SYN 15-APR-2025
DEFINITION  MoClo golden gate assembly CD part for RpaR-TYQEVV (Adam Meyer's 
            improved RpaR activator selected for dynamic range and low 
            cross-sensitivity using methods from 
            https://doi.org/10.1101/285866,)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2836)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C70m
FEATURES             Location/Qualifiers
     source          1..2836
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     19..37
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(41..84)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    87..108
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    866..886
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(892..909)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1063..1651)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1143..1162)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1822..2613,2614..2682))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2445..2464
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2683..2787)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 atgacattaa cctataaaaa taggcgtatc acgaggcaga atttcagata aaaaaaatcc
       61 ttagctttcg ctaaggatga tttctggaat tcgcggccgc ttctagagac tagtgggtct
      121 caaatgattg tgggtgaaga tcagctgtgg ggtcgtcgta cactggaatt tgttgatagc
      181 gttgaacgtc tggaagcacc ggcactgatt agccgttttg aaagcctgat tgcaagctgt
      241 ggttttaccg cctatatcat ggcaggtctg ccgagccgta atgccggtct gccggaactg
      301 accctggcaa atggttggcc tcgtgattgg tttgatctgt atgttagcga aaactttagc
      361 gcagttgatc cggttccgcg ttatggtgca accactgttc atccgtttgt ttggagtgat
      421 gcaccgtatg atcgtgaccg tgatcaggca gcacatcgtg ttatgacccg tgcagcagaa
      481 tttggtctgg ttgaaggtta ttgtattccg ctgcattacg atgatggtag cgcagcaatt
      541 agtatggctg gtgaagatcc tgatctgagt ccggcagccc gtggtgtaat gcagctggtt
      601 agcatttatg cacatagccg tctgcgtgta ctgagccgtc cgaaaccgat tcgtcgtaat
      661 cgtctgacac cgcgtgaatg tgaaattctg cagtgggcag cacagggtaa aaccgcatgg
      721 gaaattagcg ttattctgtg tattaccgaa cgcaccgtta aatttcatct gattgaagca
      781 gcacgtaaac tggatgcagc aaatcgtacc gcagcagttg caaaagcact gacactgggt
      841 ctgattcgtc tgtgaggtag agacctacta gtagcggccg ctgcaggctt cctcgctcac
      901 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
      961 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     1021 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccaca ggctccgccc
     1081 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     1141 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     1201 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     1261 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     1321 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     1381 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     1441 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     1501 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     1561 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     1621 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     1681 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     1741 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     1801 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     1861 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     1921 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgcgacccac gctcaccggc
     1981 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     2041 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     2101 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     2161 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     2221 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     2281 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     2341 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     2401 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     2461 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     2521 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     2581 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     2641 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     2701 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     2761 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     2821 agaaaccatt attatc
//
