LOCUS       sequence-236124-        5906 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Mammalian expression plasmid for SH3-5R in a vector expressing 
            mCherry..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5906)
  AUTHORS   Li P, Banjade S, Cheng HC, Kim S, Chen B, Guo L, Llaguno M, 
            Hollingsworth JV, King DS, Banani SF, Russo PS, Jiang QX, Nixon BT, 
            Rosen MK
  TITLE     Phase transitions in the assembly of multivalent signalling 
            proteins.
  JOURNAL   Nature. 2012 Mar 7;483(7389):336-40. doi: 10.1038/nature10879.
  PUBMED    22398450
REFERENCE   2  (bases 1 to 5906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2012-03-7- 7"; volume: "483"; issue: "7389"; pages:
            "336-40"
COMMENT     Sequence Label: mCherry-SH3(5R)
FEATURES             Location/Qualifiers
     source          1..5906
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      1795..1804
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1801..2511
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1946..1964)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2214..2233
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     2408..2431
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    2634..2755
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2671..2690)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2725..2744
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2762..3217)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2899..2920)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3111..3130
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3244..3348
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3350..3707
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3350..3541
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3373..3393)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3558..3693
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3742..4536
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3742..4536
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3796..3815)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4406..4425
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4724..4743)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4768..4815
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5144..5732
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5633..5652
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggactcag atctcgagct caagcttcga attctgggcc atatggacct caacatgccc
      601 gcttatgtga aatttaacta catggctgag agagaggatg aattatcatt gataaagggg
      661 acaaaggtga tcgtcatgga gaaaagcagt gatgggtggt ggcgtggtag ctacaatgga
      721 caagttggat ggttcccttc aaactatgta actgaagaag gtgacagtcc tttggcgtct
      781 ggtgcgggtg gttctgaagg tggtggttct gaaggtggta cctctggtgc gaccgacctc
      841 aacatgcccg cttatgtgaa atttaactac atggctgaga gagaggatga attatcattg
      901 ataaagggga caaaggtgat cgtcatggag aaaagcagtg atgggtggtg gcgtggtagc
      961 tacaatggac aagttggatg gttcccttca aactatgtaa ctgaagaagg tgacagtcct
     1021 ttggcatccg gagcaggagg ctccgaagga ggaggctccg agggaggaac atccggagca
     1081 acacatatgg acctcaacat gcccgcttat gtgaaattta actacatggc tgagagagag
     1141 gatgaattat cattgataaa ggggacaaag gtgatcgtca tggagaaaag cagtgatggg
     1201 tggtggcgtg gtagctacaa tggacaagtt ggatggttcc cttcaaacta tgtaactgaa
     1261 gaaggtgaca gtcctttggc gtctggtgcg ggtggttctg aaggtggtgg ttctgaaggt
     1321 ggtacctctg gtgcgaccga cctcaacatg cccgcttatg tgaaatttaa ctacatggct
     1381 gagagagagg atgaattatc attgataaag gggacaaagg tgatcgtcat ggagaaaagc
     1441 agtgatgggt ggtggcgtgg tagctacaat ggacaagttg gatggttccc ttcaaactat
     1501 gtaactgaag aaggtgacag tcctttggca tccggagcag gaggctccga aggaggaggc
     1561 tccgagggag gaacatccgg agcaacagac ctcaacatgc ccgcttatgt gaaatttaac
     1621 tacatggctg agagagagga tgaattatca ttgataaagg ggacaaaggt gatcgtcatg
     1681 gagaaaagca gtgatgggtg gtggcgtggt agctacaatg gacaagttgg atggttccct
     1741 tcaaactatg taactgaaga aggtgacagt cctttgggac gggatccacc ggtcgccacc
     1801 atggtgagca agggcgagga ggacaacatg gccatcatca aggagttcat gcgcttcaag
     1861 gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc
     1921 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc
     1981 ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac
     2041 cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc
     2101 gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac
     2161 ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta
     2221 atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc
     2281 gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgcc
     2341 gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc
     2401 aacatcaagc tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgag
     2461 cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagta aagcggccgc
     2521 gactctagat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
     2581 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     2641 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     2701 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttaaggcg
     2761 taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca
     2821 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag
     2881 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
     2941 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc
     3001 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
     3061 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
     3121 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
     3181 acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt tcggggaaat
     3241 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     3301 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa
     3361 agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     3421 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     3481 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     3541 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     3601 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     3661 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaagat cgatcaagag
     3721 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc
     3781 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
     3841 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
     3901 tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg
     3961 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
     4021 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
     4081 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
     4141 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
     4201 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
     4261 ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
     4321 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
     4381 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
     4441 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
     4501 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga
     4561 ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg
     4621 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
     4681 atctcatgct ggagttcttc gcccacccta gggggaggct aactgaaaca cggaaggaga
     4741 caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacggtg
     4801 ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc
     4861 caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc
     4921 caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag
     4981 cctcaggtta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     5041 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     5101 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     5161 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     5221 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     5281 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     5341 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     5401 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     5461 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     5521 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     5581 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     5641 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     5701 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     5761 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     5821 tggataaccg tattaccgcc atgcattagt tattaatagt aatcaattac ggggtcatta
     5881 gttcatagcc catatatgga gttccg
//
