LOCUS       sequence-236186-        9451 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Lentiviral vector for stable expression of Firefly luciferase..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9451)
  AUTHORS   Gorbea C, Mosbruger T, Nix DA, Cazalla D
  TITLE     Viral miRNA adaptor differentially recruits miRNAs to target mRNAs 
            through alternative base-pairing.
  JOURNAL   Elife. 2019 Sep 20;8. pii: 50530. doi: 10.7554/eLife.50530.
  PUBMED    31538617
REFERENCE   2  (bases 1 to 9451)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2019-09-20"; volume: "8. pii: 50530. doi"; pages: " 
            10.7554/eLife.50530"
COMMENT     Sequence Label: pLenti-TK-Firefly-cjTP53RK
FEATURES             Location/Qualifiers
     source          1..9451
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             757..2409
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(2699..2716)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2705..2929
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(3165..3209)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3394..3627
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    4120..4245
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             4292..4472
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(4473..4699)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(4727..4747)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4727..4745)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4766..4782)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4766..4782)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4779..4801)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4790..4806
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4814..4820,4821..4838,4839..4844))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4859..4880
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4997..5014)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5168..5756)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5248..5267)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5927..6718,6719..6787))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6550..6569
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6788..6892)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6918..7373)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7055..7076)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7267..7286
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7500..7522
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7514..7531
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7515..7531
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7541..7560
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7541..7559
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      7580..7715
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7755..7876
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7766..7785)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7820..7839
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             7948..8181
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(8319..8918)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8402..8422)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     8899..8918
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(8939..9438)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8987..9006)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     9380..9403
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     promoter        join(9450..9451,1..750)
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
ORIGIN
        1 tgagtcttcg gacctcgcgg gggccgctta agcggtggtt agggtttgtc tgacgcgggg
       61 ggagggggaa ggaacgaaac actctcattc ggaggcggct cggggtttgg tcttggtggc
      121 cacgggcacg cagaagagcg ccgcgatcct cttaagcacc cccccgccct ccgtggaggc
      181 gggggtttgg tcggcgggtg gtaactggcg ggccgctgac tcgggcgggt cgcgcgcccc
      241 agagtgtgac cttttcggtc tgctcgcaga cccccgggcg gcgccgccgc ggcggcgacg
      301 ggctcgctgg gtcctaggct ccatggggac cgtatacgtg gacaggctct ggagcatccg
      361 cacgactgcg gtgatattac cggagacctt ctgcgggacg agccgggtca cgcggctgac
      421 gcggagcgtc cgttgggcga caaacaccag gacggggcac aggtacacta tcttgtcacc
      481 cggaggcgcg agggactgca ggagcttcag ggagtggcgc agctgcttca tccccgtggc
      541 ccgttgctcg cgtttgctgg cggtgtcccc ggaagaaata tatttgcatg tctttagttc
      601 tatgatgaca caaaccccgc ccagcgtctt gtcattggcg aattcgaaca cgcagatgca
      661 gtcggggcgg cgcggtccca ggtccacttc gcatattaag gtgacgcgtg tggcctcgaa
      721 caccgagcga ccctgcagcg acccgcttaa ggatccatgg aagatgccaa aaacattaag
      781 aagggcccag cgccattcta cccactcgaa gacgggaccg ccggcgagca gctgcacaaa
      841 gccatgaagc gctacgccct ggtgcccggc accatcgcct ttaccgacgc acatatcgag
      901 gtggacatta cctacgccga gtacttcgag atgagcgttc ggctggcaga agctatgaag
      961 cgctatgggc tgaatacaaa ccatcggatc gtggtgtgca gcgagaatag cttgcagttc
     1021 ttcatgcccg tgttgggtgc cctgttcatc ggtgtggctg tggccccagc taacgacatc
     1081 tacaacgagc gcgagctgct gaacagcatg ggcatcagcc agcccaccgt cgtattcgtg
     1141 agcaagaaag ggctgcaaaa gatcctcaac gtgcaaaaga agctaccgat catacaaaag
     1201 atcatcatca tggatagcaa gaccgactac cagggcttcc aaagcatgta caccttcgtg
     1261 acttcccatt tgccacccgg cttcaacgag tacgacttcg tgcccgagag cttcgaccgg
     1321 gacaaaacca tcgccctgat catgaacagt agtggcagta ccggattgcc caagggcgta
     1381 gccctaccgc accgcaccgc ttgtgtccga ttcagtcatg cccgcgaccc catcttcggc
     1441 aaccagatca tccccgacac cgctatcctc agcgtggtgc catttcacca cggcttcggc
     1501 atgttcacca cgctgggcta cttgatctgc ggctttcggg tcgtgctcat gtaccgcttc
     1561 gaggaggagc tattcttgcg cagcttgcaa gactataaga ttcaatctgc cctgctggtg
     1621 cccacactat ttagcttctt cgctaagagc actctcatcg acaagtacga cctaagcaac
     1681 ttgcacgaga tcgccagcgg cggggcgccg ctcagcaagg aggtaggtga ggccgtggcc
     1741 aaacgcttcc acctaccagg catccgccag ggctacggcc tgacagaaac aaccagcgcc
     1801 attctgatca cccccgaagg ggacgacaag cctggcgcag taggcaaggt ggtgcccttc
     1861 ttcgaggcta aggtggtgga cttggacacc ggtaagacac tgggtgtgaa ccagcgcggc
     1921 gagctgtgcg tccgtggccc catgatcatg agcggctacg ttaacaaccc cgaggctaca
     1981 aacgctctca tcgacaagga cggctggctg cacagcggcg acatcgccta ctgggacgag
     2041 gacgagcact tcttcatcgt ggaccggctg aagagcctga tcaaatacaa gggctaccag
     2101 gtagccccag ccgaactgga gagcatcctg ctgcaacacc ccaacatctt cgacgccggg
     2161 gtcgccggcc tgcccgacga cgatgccggc gagctgcccg ccgcagtcgt cgtgctggaa
     2221 cacggtaaaa ccatgaccga gaaggagatc gtggactatg tggccagcca ggttacaacc
     2281 gccaagaagc tgcgcggtgg tgttgtgttc gtggacgagg tgcctaaagg actgaccggc
     2341 aagttggacg cccgcaagat ccgcgagatt ctcattaagg ccaagaaggg cggcaagatc
     2401 gccgtgtaat tctagttgtt taaacgtaga agaatgtgtc tgacagccag acacagtgaa
     2461 gctgtttttt caaagtacat ttgaagaaat gctgcaagta agagagtaga tttaagtaaa
     2521 ggtgttaaga tatttttaag tagtgtgtga tcgtgcctgg tgtgtcatta tcatctgtac
     2581 tactggagct ttctatgtat ctaagacatg ttctaggcgg aattgagtaa agttaatcaa
     2641 tgcaggtttt ctcccaggtt gtgactttct agagtcgacg ctagagctcg ctgatcagcc
     2701 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     2761 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     2821 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     2881 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
     2941 gaaagaacca gctggggctc tagggggtat cccctccccc cttttctttt gggtcccctc
     3001 ggggttggga ggtgggtctg aaacgataat ggtgaatatc cctgcctaac tctattcact
     3061 atagaaagta cagcaaaaac tattcttaaa cctaccaagc ctcctactat cattatgaat
     3121 aattttatat accacagcca atttgttatg ttaaaccaat tccacaaact tgcccattta
     3181 tctaattcca ataattcttg ttcattcttt tcttgctggt tttgcgattc ttcaattaag
     3241 gagtgtatta agcttgtgta attgttaatt tctctgtccc actccatcca ggtcgtgtga
     3301 ttccaaatct gttccagaga tttattactc caactagcat tccaaggcac agcagtggtg
     3361 caaatgagtt ttccagagca accccaaatc cccaggagct gttgatcctt taggtatctt
     3421 tccacagcca ggattcttgc ctggagctgc ttgatgcccc agactgtgag ttgcaacaga
     3481 tgctgttgcg cctcaatagc cctcagcaaa ttgttctgct gctgcactat accagacaat
     3541 aattgtctgg cctgtaccgt cagcgtcatt gacgctgcgc ccatagtgct tcctgctgct
     3601 cccaagaacc caaggaacaa agctcctatt cccactgctc ttttttctct ctgcaccact
     3661 cttctctttg ccttggtggg tgctactcct aatggttcaa tttttactac tttatattta
     3721 tataattcac ttctccaatt gtccctcata tctcctcctc caggtctgaa gatcagcggc
     3781 cgcttgctgt gcggtggtct tacttttgtt ttgctcttcc tctatcttgt ctaaagcttc
     3841 cttggtgtct tttatctcta tcctttgatg cacacaatag agggttgcta ctgtattata
     3901 taatgatcta agttcttctg atcctgtctg aagggatggt tgtagctgtc ccagtatttg
     3961 tctacagcct tctgatgttt ctaacaggcc aggattaact gcgaatcgtt ctagctccct
     4021 gcttgcccat actatatgtt ttaatttata ttttttcttt ccccctggcc ttaaccgaat
     4081 tttttcccat cgcgatctaa ttctcccccg cttaatactg acgctctcgc acccatctct
     4141 ctccttctag cctccgctag tcaaaatttt tggcgtactc accagtcgcc gcccctcgcc
     4201 tcttgccgtg cgcgcttcag caagccgagt cctgcgtcga gagagctcct ctggtttccc
     4261 tttcgctttc aagtccctgt tcgggcgcca ctgctagaga ttttccacac tgactaaaag
     4321 ggtctgaggg atctctagtt accagagtca cacaacagac gggcacacac tacttgaagc
     4381 actcaaggca agctttattg aggcttaagc agtgggttcc ctagttagcc agagagctcc
     4441 caggctcaga tctggtctaa ccagagagac ccgtttatgt atcgagctag gcacttaaat
     4501 acaatatctc tgcaatgcgg caattcagtg gttcgtccaa tccatgtcag acccgtctgt
     4561 tgccttccta ataaggcacg atcgtaccac cttacttcca ccaatcggca tgcacggtgc
     4621 tttttctctc cttgtaaggc atgttgctaa ctcatcgtta ccatgttgca agactacaag
     4681 agtattgcat aagactacat taagcttgca gctccagctt ttgttccctt tagtgagggt
     4741 taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     4801 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     4861 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     4921 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     4981 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     5041 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     5101 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     5161 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     5221 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     5281 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     5341 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     5401 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     5461 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     5521 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     5581 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     5641 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     5701 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     5761 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     5821 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     5881 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     5941 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     6001 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     6061 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     6121 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     6181 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     6241 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     6301 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     6361 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     6421 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     6481 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     6541 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     6601 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     6661 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     6721 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     6781 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     6841 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     6901 cccgaaaagt gccacctaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
     6961 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
     7021 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
     7081 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
     7141 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
     7201 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
     7261 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
     7321 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc
     7381 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
     7441 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
     7501 ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg taatacgact cactataggg
     7561 cgaattgggt acgtccctag gcctccaaaa aagcctcctc actacttctg gaatagctca
     7621 gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga
     7681 gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg cgggatagct
     7741 agagccagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg
     7801 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
     7861 ctgcaataaa caagttcctc tcactctctg atattcattt ctttgcaagt tataaatact
     7921 gaataataag atgacatgaa ctactactgc tagagatttt ccacactgac taaaagggtc
     7981 tgagggatct ctagttacca gagtcacaca acagacgggc acacactact tgaagcactc
     8041 aaggcaagct ttattgaggc ttaagcagtg ggttccctag ttagccagag agctcccagg
     8101 ctcagatctg gtctaaccag agagacccag tacaagcaaa aagcagatct tgtcttcgtt
     8161 gggagtgagc tagcccttcc agtcccccct tttcttttaa aaagtggcta agatctacag
     8221 ctgccttgta agtcattggt cttaaaggta ccgggccctc gagatgcatg gggtcgtgcg
     8281 ctcctttcgg tcgggcgctg cgggtcgtgg ggcgggcgtc aggcaccggg cttgcgggtc
     8341 atgcaccagg tgcgcggtcc ttcgggcacc tcgacgtcgg cggtgacggt gaagccgagc
     8401 cgctcgtaga aggggaggtt gcggggcgcg gaggtctcca ggaaggcggg caccccggcg
     8461 cgctcggccg cctccactcc ggggagcacg acggcgctgc ccagaccctt gccctggtgg
     8521 tcgggcgaga cgccgacggt ggccaggaac cacgcgggct ccttgggccg gtgcggcgcc
     8581 aggaggcctt ccatctgttg ctgcgcggcc agccgggaac cgctcaactc ggccatgcgc
     8641 gggccgatct cggcgaacac cgcccccgct tcgacgctct ccggcgtggt ccagaccgcc
     8701 accgcggcgc cgtcgtccgc gacccacacc ttgccgatgt cgagcccgac gcgcgtgagg
     8761 aagagttctt gcagctcggt gacccgctcg atgtggcggt ccggatcgac ggtgtggcgc
     8821 gtggcggggt agtcggcgaa cgcggcggcg agggtgcgta cggccctggg gacgtcgtcg
     8881 cgggtggcga ggcgcaccgt gggcttgtac tcggtcatgg taagcttggg ctgcaggtcg
     8941 aaaggcccgg agatgaggaa gaggagaaca gcgcggcaga cgtgcgcttt tgaagcgtgc
     9001 agaatgccgg gcctccggag gaccttcggg cgcccgcccc gcccctgagc ccgcccctga
     9061 gcccgccccc ggacccaccc cttcccagcc tctgagccca gaaagcgaag gagcaaagct
     9121 gctattggcc gctgccccaa aggcctaccc gcttccattg ctcagcggtg ctgtccatct
     9181 gcacgagact agtgagacgt gctacttcca tttgtcacgt cctgcacgac gcgagctgcg
     9241 gggcgggggg gaacttcctg actaggggag gagtagaagg tggcgcgaag gggccaccaa
     9301 agaacggagc cggttggcgc ctaccggtgg atgtggaatg tgtgcgaggc cagaggccac
     9361 ttgtgtagcg ccaagtgccc agcggggctg ctaaagcgca tgctccagac tgccttggga
     9421 aaagcgcctc ccctacccgg tagaattgaa a
//
