LOCUS       sequence-236887-        2182 bp    DNA     circular SYN 15-APR-2025
DEFINITION  MoClo golden gate assembly AB part for PlacO (common LacI-repressed 
            promoter; see DOI: 10.1126/science.1256272). Please see Supplemental
            Documents for annotated Genbank file..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2182)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: P32m
FEATURES             Location/Qualifiers
     source          1..2182
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     2..20
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(24..67)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    70..91
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     protein_bind    112..128
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    135..151
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    195..215
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(221..238)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(392..980)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(472..491)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1151..1942,1943..2011))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1774..1793
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2012..2116)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaataggcgt atcacgaggc agaatttcag ataaaaaaaa tccttagctt tcgctaagga
       61 tgatttctgg aattcgcggc cgcttctaga gactagtggg tctcaggaga attgtgagcg
      121 gataacaatt gacattgtga gcggataaca agatactgag cacatcagca ggacgcactg
      181 acctactaga gacctactag tagcggccgc tgcaggcttc ctcgctcact gactcgctgc
      241 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
      301 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
      361 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccacag gctccgcccc cctgacgagc
      421 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
      481 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
      541 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
      601 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
      661 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
      721 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
      781 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
      841 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
      901 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
      961 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     1021 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     1081 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     1141 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     1201 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     1261 catctggccc cagtgctgca atgataccgc gcgacccacg ctcaccggct ccagatttat
     1321 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     1381 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     1441 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     1501 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     1561 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     1621 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     1681 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     1741 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     1801 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     1861 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     1921 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     1981 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     2041 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     2101 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
     2161 ttatcatgac attaacctat aa
//
