LOCUS       sequence-237087-       11903 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Targets jRGECO1a to spines in living neurons..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11903)
  AUTHORS   Fan LZ, Nehme R, Adam Y, Jung ES, Wu H, Eggan K, Arnold DB, Cohen AE
  TITLE     All-optical synaptic electrophysiology probes mechanism of 
            ketamine-induced disinhibition.
  JOURNAL   Nat Methods. 2018 Oct;15(10):823-831. doi: 
            10.1038/s41592-018-0142-8. Epub 2018 Oct 1.
  PUBMED    30275587
REFERENCE   2  (bases 1 to 11903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-018-0142-8"; journalName: "Nat Methods"; date: 
            "2018-10"; volume: "15"; issue: "10"; pages: "823-831"
COMMENT     Sequence Label: LZF97_hSyn-spine-jRGECO1a
FEATURES             Location/Qualifiers
     source          1..11903
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2632..3079
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     protein_bind    3091..3102
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     CDS             3178..3195
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             3199..3231
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(3235..3243,3244..3258)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             3286..3342
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKAGHAVRAIGRLSS"
     primer_bind     4038..4057
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             6220..6561
                     /codon_start=1
                     /product="zinc-finger domain from the CCR5-224 (+) 
                     zinc-finger nuclease that recognizes the left half-site 
                     target sequence in the human CCR5 gene (Perez et al., 2008;
                     Gross et al., 2013)"
                     /label=CCR5TC
                     /translation="FQCRICMRNFSDRSNLSRHIRTHTGEKPFACDICGRKFAISSNLN
                     SHTKIHTGSQKPFQCRICMRNFSRSDNLARHIRTHTGEKPFACDICGRKFATSGNLTRH
                     TKIHLRGSQL"
     misc_feature    6595..7183
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6648..6668)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7066..7077)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7186..7202)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7187..7203)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7708..7888
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7914..7931)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7920..8144
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8190..8618
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8277..8296)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8487..8508
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8627..8647)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8632..8961
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8812..8947
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8874..8893
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9009..9056
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9075..9449
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9579..9700
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9616..9635)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9670..9689
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9749..9765)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9749..9765)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9762..9784)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9773..9789
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9797..9803,9804..9821,9822..9827))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9842..9863
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9980..9997)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10151..10739)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10231..10250)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10910..11701,11702..11770))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11533..11552
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11771..11875)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta agtgtctaga ctgcagaggg ccctgcgtat gagtgcaagt
     2641 gggttttagg accaggatga ggcggggtgg gggtgcctac ctgacgaccg accccgaccc
     2701 actggacaag cacccaaccc ccattcccca aattgcgcat cccctatcag agagggggag
     2761 gggaaacagg atgcggcgag gcgcgtgcgc actgccagct tcagcaccgc ggacagtgcc
     2821 ttcgcccccg cctggcggcg cgcgccaccg ccgcctcagc actgaaggcg cgctgacgtc
     2881 actcgccggt cccccgcaaa ctccccttcc cggccacctt ggtcgcgtcc gcgccgccgc
     2941 cggcccagcc ggaccgcacc acgcgaggcg cgagataggg gggcacgggc gcgaccatct
     3001 gcgctgcggc gccggcgact cagcgctgcc tcagtctgcg gtgggcagcg gaggagtcgt
     3061 gtcgtgcctg agagcgcagt cgagaaggat gtcatcctca tcgccgccac catgctgcag
     3121 aacgagcttg ctcttaagtt ggctggactt gatattaaca agactggagg aggttctcat
     3181 catcatcatc atcatggtat ggctagcatg actggtggac agcaaatggg tcgggatctg
     3241 tacgacgatg acgataagga tctcgcaaca atggtcgact catcacgtcg taagtggaat
     3301 aaggcaggtc acgcagtcag agctataggt cggctgagct cacccgtggt ttccgagcgg
     3361 atgtaccccg aggacggcgc cctgaagagc gagatcaaga aggggctgag gctgaaggac
     3421 ggcggccact acgccgccga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg
     3481 cccggcgcct acatcgtgga catcaagttg gacatcgtgt cccacaacga ggactacacc
     3541 atcgtggaac agtgcgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg
     3601 tacaagggag gtacaggcgg gagtctggtg agcaagggcg aggaggataa catggccatc
     3661 atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc
     3721 gagatcgagg gcgagggcga gggccgcccc tacgaggcct ttcagaccgc taagctgaag
     3781 gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac
     3841 ggctccaagg cctacattaa gcacccagcc gacatccccg actacttcaa gctgtccttc
     3901 cccgagggct tcaggtggga gcgcgtgatg aacttcgagg acggcggcat tattcacgtg
     3961 aaccaggact cctccctgca ggacggcgta ttcatctaca aggtgaagct gcgcggcacc
     4021 aacttccccc ccgacggccc cgtaatgcag aagaagacca tgggctggga ggctacgcgt
     4081 gacgacctga ctgaagagca gatcgcagaa tttaaagagg ctttctccct atttgacaag
     4141 gacggggatg ggacgataac aaccaaggag cttgggacgg tgttccgatc gctggggcag
     4201 aaccccacag aagcagagct gcaggacatg atcaatgaag tagatgccga cggtgacggc
     4261 acattcgact tccctgagtt cctgacgatg atggcaagaa aaatgaatga cacagacagt
     4321 gaagaggaaa ttcgcgaagc gttccgcgtg tttgataagg acggcaatgg ctacatcggc
     4381 gcagcagagc ttcgccacgt gatgacagac cttggagaga agttaacaga tgaggaggtt
     4441 gatgaaatga tcagggtagc agacatcgat ggggatggtc aggtaaacta cgaagagttt
     4501 gtacaaatga tgacagcgaa gggtggaggt tctggtggag gttctggagg tggatcagga
     4561 ggaggttcga gatctaagct ctcaggtggc ggagggagcg gcggaggtgg ctctggaggt
     4621 ggtggctcag ctgaagcatg gtacaatctg ggaaacgctt attacaagca gggcgactat
     4681 caaaaagcca ttgagtatta ccaaaaggct cttgagctag accctaataa cgccgaagcg
     4741 tggtataacc taggcaacgc atattacaag cagggagact accaaaaggc aatcgaatat
     4801 taccagaagg cactcgaact ggacccaaat aacgcagagg cttggtataa tctgggaaac
     4861 gcctactata aacagggtga ctaccaaaag gcgatagagg actatcagaa ggccttggag
     4921 ctcgatccga acaatctgca acgtagtgct ggtggcggag ggtctggtgg aggaggctca
     4981 ggaggaggtg gcgcatcaaa cgttgccaag tacgggcacc ttgaagccag ccctgatgat
     5041 gggcagaatc agttcagtga tatcattaag tccagatctg atgaacacaa cgatgtacag
     5101 aagaaaacct ttaccaaatg gataaacgct cgattttcaa agagtgggaa accacccatc
     5161 aatgatatgt tctcagacct caaagatggg agaaagctct tggatcttct agaaggcctc
     5221 acaggaacat cattgccaaa ggaacgtggt tccacaaggg tgcatgcctt aaataatgtc
     5281 aaccgggtgc tgcaggtttt acatcagaac aatgtggagc tggtgaatat cggaggcact
     5341 gacattgtgg atggaaatcc caagctgacg ctgggtttgc tgtggagtat tattctgcac
     5401 tggcaggtga aggatgtcat gaaagatatc atgtcagacc tgcagcagac gaacagcgag
     5461 aagatcctgc tgagctgggt acggcagacc accaggccct acagccaagt caacgtcctc
     5521 aacttcacca ccagctggac agatggactc gcattcaacg ccgtgctcca ccggcacaaa
     5581 ccagatctct tcagctggga cagagtggtc aaaatgtccc caactgagag acttgaacat
     5641 gcttttagca aggcccatac ttatttgggg attgaaaaac ttctggatcc tgaagatgtt
     5701 gccgtgcagc tccctgacaa gaaatccata attatgtatt taacgtctct gtttgaggtg
     5761 cttcctcagc aagtcactat agaccaattg aagctctcag gtggtggagg gagcggcgga
     5821 ggtggctctg gaggtggtgg ctcagctgaa gcatggtaca atctgggaaa cgcttattac
     5881 aagcagggcg actatcaaaa agccattgag tattaccaaa aggctcttga gctagaccct
     5941 aataacgccg aagcgtggta taacctaggc aacgcatatt acaagcaggg agactaccaa
     6001 aaggcaatcg aatattacca gaaggcactc gaactggacc caaataacgc agaggcttgg
     6061 tataatctgg gaaacgccta ctataaacag ggtgactacc aaaaggcgat agaggactat
     6121 cagaaggcct tggagctcga tccgaacaat ctgcaacgta gtgctggtgg cggagggtct
     6181 ggtggaggag gctcaggagg aggtggcgca tcaaacgaat tccagtgccg gatctgcatg
     6241 cggaacttca gcgaccggtc caacctgagc aggcacatca gaacccacac cggagaaaag
     6301 cccttcgcct gcgacatttg cggccggaag ttcgccatca gcagcaacct gaacagccac
     6361 accaagatcc acactggcag ccagaaacct ttccagtgca gaatttgtat gagaaacttt
     6421 agcagaagcg acaacctggc cagacacatc cggacacata ctggtgaaaa accttttgcc
     6481 tgtgatatct gtggcagaaa gtttgccacc tccggcaatc tgacccggca cacaaagatt
     6541 cacctgcggg gcagccagct atcgatttaa gaattcgata tcaagcttat cgataatcaa
     6601 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     6661 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     6721 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     6781 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     6841 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     6901 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     6961 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     7021 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     7081 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     7141 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgacct
     7201 cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat
     7261 tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta
     7321 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     7381 gggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc
     7441 taccacacac aaggctactt ccctgattgg cagaactaca caccagggcc agggatcaga
     7501 tatccactga cctttggatg gtgctacaag ctagtaccag ttgagcaaga gaaggtagaa
     7561 gaagccaatg aaggagagaa cacccgcttg ttacaccctg tgagcctgca tgggatggat
     7621 gacccggaga gagaagtatt agagtggagg tttgacagcc gcctagcatt tcatcacatg
     7681 gcccgagagc tgcatccgga ctgtactggg tctctctggt tagaccagat ctgagcctgg
     7741 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     7801 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     7861 ttttagtcag tgtggaaaat ctctagcagg gcccgtttaa acccgctgat cagcctcgac
     7921 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     7981 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     8041 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     8101 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
     8161 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc
     8221 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     8281 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     8341 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     8401 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     8461 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     8521 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     8581 aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag
     8641 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     8701 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     8761 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     8821 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     8881 gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt
     8941 ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat
     9001 cagcacgtgt tgacaattaa tcatcggcat agtatatcgg catagtataa tacgacaagg
     9061 tgaggaacta aaccatggcc aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg
     9121 tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg
     9181 acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg gtccaggacc
     9241 aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg cggcctggac gagctgtacg
     9301 ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc ctccgggccg gccatgaccg
     9361 agatcggcga gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg
     9421 tgcacttcgt ggccgaggag caggactgac acgtgctacg agatttcgat tccaccgccg
     9481 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
     9541 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata
     9601 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     9661 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga
     9721 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     9781 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     9841 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     9901 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     9961 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
    10021 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
    10081 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
    10141 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
    10201 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
    10261 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
    10321 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
    10381 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
    10441 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
    10501 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
    10561 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
    10621 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
    10681 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
    10741 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
    10801 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
    10861 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
    10921 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
    10981 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
    11041 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
    11101 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
    11161 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
    11221 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
    11281 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
    11341 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
    11401 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
    11461 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
    11521 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
    11581 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
    11641 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
    11701 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
    11761 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
    11821 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
    11881 ttccccgaaa agtgccacct gac
//
