LOCUS       sequence-237437-        8823 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Gateway entry vector with PmCDA1 and pcoCas9D10A-x3.7 for C-T base 
            editing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8823)
  AUTHORS   Zhong Z, Sretenovic S, Ren Q, Yang L, Bao Y, Qi C, Yuan M, He Y, Liu
            S, Liu X, Wang J, Huang L, Wang Y, Baby D, Wang D, Zhang T, Qi Y, 
            Zhang Y
  TITLE     Improving Plant Genome Editing with High-Fidelity xCas9 and 
            Non-canonical PAM-Targeting Cas9-NG.
  JOURNAL   Mol Plant. 2019 Mar 27. pii: S1674-2052(19)30124-8. doi: 
            10.1016/j.molp.2019.03.011.
  PUBMED    30928637
REFERENCE   2  (bases 1 to 8823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Plant. 
            2019 Mar 27. pii: S1674-2052(19)30124-8. doi: 
            10.1016/j.molp.2019.03.011."
COMMENT     Sequence Label: pYPQ256-x3.7
FEATURES             Location/Qualifiers
     source          1..8823
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             358..1149
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1242..1830
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1731..1750
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1984..2001
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2160..2187)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2279..2365)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2414..2436
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2428..2445
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2429..2445
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2461..2560
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2582..2629
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     CDS             2636..2656
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2681..6781
                     /codon_start=1
                     /product="Streptococcus pyogenes Cas9 mutant with broad PAM
                     compatibility (Hu et al., 2018)"
                     /label=xCas9-3.7
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDTKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKLYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGIIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEKVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGDQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFIQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGVLQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6782..6829
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             7046..7111
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             7745..7765
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7772..8023
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="MTNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHT
                     AYDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             8024..8077
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     terminator      8273..8525
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    8570..8693
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(8710..8729)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8711..8729)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8734..8750)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8734..8750)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tggaccagcc aggacagaaa tgcctcgact tcgctgctac ccaaggttgc cgggtgacgc
       61 acaccgtgga aacggatgaa ggcacgaacc cagtggacat aagcctgttc ggttcgtaag
      121 ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc
      181 ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag
      241 tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta
      301 tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacatcatg
      361 agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag
      421 cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc
      481 ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca
      541 acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag
      601 attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat
      661 ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc
      721 ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat
      781 agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat
      841 ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc
      901 gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa
      961 atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag
     1021 cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg
     1081 cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc
     1141 ggcaaataac cctcgagcca cccatgacca aaatccctta acgtgagtta cgcgtcgttc
     1201 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     1261 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     1321 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     1381 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     1441 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     1501 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     1561 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     1621 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     1681 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     1741 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     1801 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     1861 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     1921 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     1981 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     2041 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     2101 agtgagcgca acgcaattaa tacgcgtacc gcgagccagg aagagtttgt agaaacgcaa
     2161 aaaggccatc cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc
     2221 gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat
     2281 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt
     2341 ccgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc gttaacgctt
     2401 gcatggatgt tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc
     2461 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
     2521 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgaattcgc ccttcaccat
     2581 ggattacaag gatgatgatg ataaggatta caaggatgat gatgataaga tggctccaaa
     2641 gaagaagaga aaggttggaa tccacggagt tccagctgct gataagaagt actctatcgg
     2701 acttgccatc ggaaccaact ctgttggatg ggctgttatc accgatgagt acaaggttcc
     2761 atctaagaag ttcaaggttc ttggaaacac cgatagacac tctatcaaga agaaccttat
     2821 cggtgctctt cttttcgatt ctggagagac cgctgaggct accagattga agagaaccgc
     2881 tagaagaaga tacaccagaa gaaagaacag aatctgctac cttcaggaaa tcttctctaa
     2941 cgagatggct aaggttgatg attctttctt ccacagactt gaggagtctt tccttgttga
     3001 ggaggataag aagcacgaga gacacccaat cttcggaaac atcgttgatg aggttgctta
     3061 ccacgagaag tacccaacca tctaccacct tagaaagaag ttggttgatt ctaccgataa
     3121 ggctgatctt agacttatct accttgctct tgctcacatg atcaagttca gaggacactt
     3181 ccttatcgag ggagacctta acccagataa ctctgatgtt gataagttgt tcatccagct
     3241 tgttcagacc tacaaccagc ttttcgagga gaacccaatc aacgcttctg gagttgatgc
     3301 taaggctatc ctttctgcta gactttctaa gtctcgtaga cttgagaacc ttatcgctca
     3361 gcttccagga gagaagaaga acggactttt cggaaacctt atcgctcttt ctcttggact
     3421 taccccaaac ttcaagtcta acttcgatct tgctgaggat accaagttgc agctttctaa
     3481 ggatacctac gatgatgatc ttgataacct tcttgctcag atcggagatc agtacgctga
     3541 tcttttcctt gctgctaaga acctttctga tgctatcctt ctttctgaca tccttagagt
     3601 taacaccgag atcaccaagg ctccactttc tgcttctatg atcaagctct acgatgagca
     3661 ccaccaggat cttacccttt tgaaggctct tgttagacag cagcttccag agaagtacaa
     3721 ggaaatcttc ttcgatcagt ctaagaacgg atacgctgga tacatcgatg gaggagcttc
     3781 tcaggaggag ttctacaagt tcatcaagcc aatccttgag aagatggatg gaaccgagga
     3841 gcttcttgtt aagttgaaca gagaggatct tcttagaaag cagagaacct tcgataacgg
     3901 aatcatccca caccagatcc accttggaga gcttcacgct atccttcgta gacaggagga
     3961 tttctaccca ttcttgaagg ataacagaga gaagatcgag aagatcctta ccttcagaat
     4021 cccatactac gttggaccac ttgctagagg aaactctcgt ttcgcttgga tgaccagaaa
     4081 gtctgaggag accatcaccc cttggaactt cgagaaggtt gttgataagg gagcttctgc
     4141 tcagtctttc atcgagagaa tgaccaactt cgataagaac cttccaaacg agaaggttct
     4201 tccaaagcac tctcttcttt acgagtactt caccgtttac aacgagctta ccaaggttaa
     4261 gtacgttacc gagggaatga gaaagccagc tttcctttct ggagaccaga agaaggctat
     4321 cgttgatctt cttttcaaga ccaacagaaa ggttaccgtt aagcagttga aggaggatta
     4381 cttcaagaag atcgagtgct tcgattctgt tgaaatctct ggagttgagg atagattcaa
     4441 cgcttctctt ggaacctacc acgatctttt gaagatcatc aaagataagg acttccttga
     4501 taacgaagag aacgaggata tcctcgagga catcgttctt accttgaccc ttttcgagga
     4561 tagagagatg atcgaggaga gactcaagac ctacgctcac cttttcgatg ataaggttat
     4621 gaagcagttg aagagaagaa gatacaccgg atggggtaga ctttctcgta agttgatcaa
     4681 cggaatcaga gataagcagt ctggaaagac catccttgat ttcttgaagt ctgatggatt
     4741 cgctaacaga aacttcatcc agcttatcca cgatgattct cttaccttca aggaggacat
     4801 ccagaaggct caggtttctg gacagggaga ttctcttcac gagcacatcg ctaaccttgc
     4861 tggatctcca gctatcaaga agggaatcct tcagaccgtt aaggttgttg atgagcttgt
     4921 taaggttatg ggtagacaca agccagagaa catcgttatc gagatggcta gagagaacca
     4981 gaccacccag aagggacaga agaactctcg tgagagaatg aagagaatcg aggagggaat
     5041 caaggagctt ggatctcaaa tcttgaagga gcacccagtt gagaacaccc agcttcagaa
     5101 cgagaagttg tacctttact accttcagaa cggaagagat atgtacgttg atcaggagct
     5161 tgacatcaac agactttctg attacgatgt tgatcacatc gttccacagt ctttcttgaa
     5221 ggatgattct atcgataaca aggttcttac ccgttctgat aagaacagag gaaagtctga
     5281 taacgttcca tctgaggagg ttgttaagaa gatgaagaac tactggagac agcttcttaa
     5341 cgctaagttg atcacccaga gaaagttcga taaccttacc aaggctgaga gaggaggact
     5401 ttctgagctt gataaggctg gattcatcaa gagacagctt gttgagacca gacagatcac
     5461 caagcacgtt gctcagatcc ttgattctcg tatgaacacc aagtacgatg agaacgataa
     5521 gttgatcaga gaggttaagg ttatcacctt gaagtctaag ttggtttctg atttcagaaa
     5581 ggatttccag ttctacaagg ttagagagat caacaactac caccacgctc acgatgctta
     5641 ccttaacgct gttgttggaa ccgctcttat caagaagtac ccaaagttgg agtctgagtt
     5701 cgtttacgga gattacaagg tttacgatgt tagaaagatg atcgctaagt ctgagcagga
     5761 gatcggaaag gctaccgcta agtacttctt ctactctaac atcatgaact tcttcaagac
     5821 cgagatcacc cttgctaacg gagagatcag aaagagacca cttatcgaga ccaacggaga
     5881 gaccggagag atcgtttggg ataagggaag agatttcgct accgttagaa aggttctttc
     5941 tatgccacag gttaacatcg ttaagaaaac cgaggttcag accggaggat tctctaagga
     6001 gtctatcctt ccaaagagaa actctgataa gttgatcgct agaaagaagg attgggaccc
     6061 aaagaagtac ggaggattcg attctccaac cgttgcttac tctgttcttg ttgttgctaa
     6121 ggttgagaag ggaaagtcta agaagttgaa gtctgttaag gagcttcttg gaatcaccat
     6181 catggagcgt tcttctttcg agaagaaccc aatcgatttc cttgaggcta agggatacaa
     6241 ggaggttaag aaggatctta tcatcaagtt gccaaagtac tctcttttcg agcttgagaa
     6301 cggaagaaag agaatgcttg cttctgctgg agtgcttcag aagggaaacg agcttgctct
     6361 tccatctaag tacgttaact tcctttacct tgcttctcac tacgagaagt tgaagggatc
     6421 tccagaggat aacgagcaga agcagctttt cgttgagcag cacaagcact accttgatga
     6481 gatcatcgag caaatctctg agttctctaa gagagttatc cttgctgatg ctaaccttga
     6541 taaggttctt tctgcttaca acaagcacag agataagcca atcagagagc aggctgagaa
     6601 catcatccac cttttcaccc ttaccaacct tggtgctcca gctgctttca agtacttcga
     6661 taccaccatc gatagaaaaa gatacacctc taccaaggag gttcttgatg ctacccttat
     6721 ccaccagtct atcaccggac tttacgagac cagaatcgat ctttctcagc ttggaggaga
     6781 taagagacca gctgctacca agaaggctgg acaggctaag aagaagaagg gagacgacgg
     6841 aggtggcggt acaggagggg gtgggtccgc tgagtatgtc agggcgttgt tcgacttcaa
     6901 tggaaacgac gaggaagatc tgccttttaa aaagggagat attctcagga tcagagataa
     6961 gccggaagaa caatggtgga acgctgaaga ctctgaaggt aagagaggta tgattcttgt
     7021 cccctacgtc gagaagtatt cgggtgacta taaagaccac gatggagatt ataaggacca
     7081 cgatatagat tataaggatg atgatgataa gagcggaatg accgatgcag agtacgtcag
     7141 gattcatgag aaacttgaca tctacacgtt taagaaacag tttttcaaca acaaaaaatc
     7201 tgttagtcac cgctgttacg tgctgttcga attgaaacgc agaggtgaga ggagagcctg
     7261 cttttggggc tatgccgtca acaagccgca aagcggcaca gaaaggggca ttcacgcgga
     7321 gatatttagc attagaaagg tcgaggaata ccttcgggat aatcccgggc aattcactat
     7381 caattggtac tcttcatggt ccccgtgtgc agattgcgct gaaaagatac tggagtggta
     7441 taatcaagaa ctcagaggaa acggtcacac cctcaagatt tgggcttgca agctttacta
     7501 cgagaaaaat gcaaggaacc agatcggcct ctggaacttg cgcgacaacg gcgtggggtt
     7561 gaatgtgatg gtctcggagc attaccagtg ctgccggaag atattcattc agtcgtcaca
     7621 taatcaattg aacgagaata ggtggctcga aaaaaccctg aagcgggccg agaagtggag
     7681 gagtgaactc tcgataatga tccaggttaa aatactgcat actaccaaat ctccggcggt
     7741 gggaccgaag aagaagcgca aggtggggac catgactaat ctctcagata taatcgagaa
     7801 ggaaacagga aagcaactgg tcatccaaga atcgattttg atgcttcccg aagaagtcga
     7861 agaagttata ggaaataagc ccgagtctga catactggtt cacacagcgt acgatgaaag
     7921 tacggacgag aatgtcatgt tgctgacatc ggacgcacct gaatacaagc cttgggctct
     7981 ggtcatacaa gatagtaacg gagaaaataa gattaaaatg cttgaaggaa ggggctccct
     8041 gctgacatgt ggagatgtgg aagagaatcc aggaccatcc aggatgatag agcaagatgg
     8101 ttgtacccaa gtgctgcggc ccctcggatg gcgggggtac agcgccatga ctggccataa
     8161 tagacagtcc gctgctctca tgcctccatg cagcggatgt cagaggaggg gagctaggtt
     8221 cttcctgtct agacctacct gccccgtgcc ctagtggcgg ccgccgacgt ccgatcgttc
     8281 aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat
     8341 catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt
     8401 atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga
     8461 aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact
     8521 agatcgggaa ttgatccccc ctcgacagct tccggaaagg gcgaattcgc aactttgtat
     8581 acaaaagttg aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg
     8641 cataaaaaac agactacata atactgtaaa acacaacata tccagtcact atgccatcca
     8701 gctgatatcc cctatagtga gtcgtattac atggtcatag ctgtttcctg gcagctctgg
     8761 cccgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgcctc
     8821 ctc
//
