LOCUS       sequence-237608-       12549 bp    DNA     circular SYN 14-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12549)
  AUTHORS   McKenzie GJ, Craig NL
  TITLE     Fast, easy and efficient: site-specific insertion of transgenes into
            enterobacterial chromosomes using Tn7 without need for selection of 
            the insertion event.
  JOURNAL   BMC Microbiol. 2006  . 6():39.
  PUBMED    16646962
REFERENCE   2  (bases 1 to 12549)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Microbiol. 2006  . 6():39."
COMMENT     Sequence Label: pGRG36
FEATURES             Location/Qualifiers
     source          1..12549
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(223..1101)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1128..1412
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1335..1354
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     primer_bind     complement(8103..8120)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      8273..8359
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      8451..8478
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        8497..8588
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8589..8657,8658..9449)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8807..8826)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     complement(9578..9597)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     oriT            9620..9729
                     /note="incP origin of transfer"
     primer_bind     complement(9767..9786)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     rep_origin      10469..10691
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             10739..11689
                     /codon_start=1
                     /gene="rep101 encoding an A56V mutation"
                     /product="temperature-sensitive version of the RepA protein
                     needed for replication with the pSC101 origin (Armstrong et
                     al., 1984)"
                     /label=Rep101(Ts)
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYVQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     mobile_element  12122..12287
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 attctgtcta gtttaagact ttattgtcat agtttagatc tattttgttc agtttaagac
       61 tttattgtcc gcccacaggc cacgatgcgt ccggcgtaga ggatctgctc atgtttgaca
      121 gcttatcatc gatgcataat gtgcctgtca aatggacgaa gcagggattc tgcaaaccct
      181 atgctactcc gtcaagccgt caattgtctg attcgttacc aattatgaca acttgacggc
      241 tacatcattc actttttctt cacaaccggc acggaactcg ctcgggctgg ccccggtgca
      301 ttttttaaat acccgcgaga aatagagttg atcgtcaaaa ccaacattgc gaccgacggt
      361 ggcgataggc atccgggtgg tgctcaaaag cagcttcgcc tggctgatac gttggtcctc
      421 gcgccagctt aagacgctaa tccctaactg ctggcggaaa agatgtgaca gacgcgacgg
      481 cgacaagcaa acatgctgtg cgacgctggc gatatcaaaa ttgctgtctg ccaggtgatc
      541 gctgatgtac tgacaagcct cgcgtacccg attatccatc ggtggatgga gcgactcgtt
      601 aatcgcttcc atgcgccgca gtaacaattg ctcaagcaga tttatcgcca gcagctccga
      661 atagcgccct tccccttgcc cggcgttaat gatttgccca aacaggtcgc tgaaatgcgg
      721 ctggtgcgct tcatccgggc gaaagaaccc cgtattggca aatattgacg gccagttaag
      781 ccattcatgc cagtaggcgc gcggacgaaa gtaaacccac tggtgatacc attcgcgagc
      841 ctccggatga cgaccgtagt gatgaatctc tcctggcggg aacagcaaaa tatcacccgg
      901 tcggcaaaca aattctcgtc cctgattttt caccaccccc tgaccgcgaa tggtgagatt
      961 gagaatataa cctttcattc ccagcggtcg gtcgataaaa aaatcgagat aaccgttggc
     1021 ctcaatcggc gttaaacccg ccaccagatg ggcattaaac gagtatcccg gcagcagggg
     1081 atcattttgc gcttcagcca tacttttcat actcccgcca ttcagagaag aaaccaattg
     1141 tccatattgc atcagacatt gccgtcactg cgtcttttac tggctcttct cgctaaccaa
     1201 accggtaacc ccgcttatta aaagcattct gtaacaaagc gggaccaaag ccatgacaaa
     1261 aacgcgtaac aaaagtgtct ataatcacgg cagaaaagtc cacattgatt atttgcacgg
     1321 cgtcacactt tgctatgcca tagcattttt atccataaga ttagcggatc ctacctgacg
     1381 ctttttatcg caactctcta ctgtttctcc atacccgttt ttttgggcta gcgaattcga
     1441 gctcggtacc caagtcttaa actagacaga atagttgtaa actgaaatca gtccagttat
     1501 gctgtgaaaa agcatactgg acttttgtta tggctaaagc aaactcttca ttttctgaag
     1561 tgcaaattgc ccgtcgtatt aaagaggggc gtggccaagg gcatggtaaa gactatattc
     1621 catggctaac agtacaagaa gttccttctt caggtcgttc ccaccgtatt tattctcata
     1681 agacgggacg agtccatcat ttgctatctg acttagagct tgctgttttt ctcagtcttg
     1741 agtgggagag cagcgtgcta gatatacgcg agcagttccc cttattacct agtgatacca
     1801 ggcagattgc aatagatagt ggtattaagc atcctgttat tcgtggtgta gatcaggtta
     1861 tgtctactga ttttttagtg gactgcaaag atggtccttt tgagcagttt gctattcaag
     1921 tcaaacctgc agcagcctta caagacgagc gtaccttaga aaaactagaa ctagagcgtc
     1981 gctattggca gcaaaagcaa attccttggt tcatttttac tgataaagaa ataaatcccg
     2041 tagtaaaaga aaatattgaa tggctttatt cagtgaaaac agaagaagtt tctgcggagc
     2101 ttttagcaca actatcccca ttggcccata tcctgcaaga aaaaggagat gaaaacatta
     2161 tcaatgtctg taagcaggtt gatattgctt atgatttgga gttaggcaaa acattgagtg
     2221 agatacgagc cttaaccgca aatggtttta ttaagttcaa tatttataag tctttcaggg
     2281 caaataagtg tgcagatctc tgtattagcc aagtagtgaa tatggaggag ttgcgctatg
     2341 tggcaaatta atgaggttgt gctatttgat aatgatccgt atcgcatttt ggctatagag
     2401 gatggccaag ttgtctggat gcaaataagc gctgataaag gagttccaca agctagggct
     2461 gagttgttgc taatgcagta tttagatgaa ggccgcttag ttagaactga tgacccttat
     2521 gtacatcttg atttagaaga gccgtctgta gattctgtca gcttccagaa gcgcgaggag
     2581 gattatcgaa aaattcttcc tattattaat agtaaggatc gtttcgaccc taaagtcaga
     2641 agcgaactcg ttgagcatgt ggtccaagaa cataaggtta ctaaggctac agtttataag
     2701 ttgttacgcc gttactggca gcgtggtcaa acgcctaatg cattaattcc tgactacaaa
     2761 aacagcggtg caccagggga aagacgttca gcgacaggaa cagcaaagat tggccgagcc
     2821 agagaatatg gtaagggtga aggaaccaag gtaacgcccg agattgaacg cctttttagg
     2881 ttgaccatag aaaagcacct gttaaatcaa aaaggtacaa agaccaccgt tgcctataga
     2941 cgatttgtgg acttgtttgc tcagtatttt cctcgcattc cccaagagga ttacccaaca
     3001 ctacgtcagt ttcgttattt ttatgatcga gaatacccta aagctcagcg cttaaagtct
     3061 agagttaaag caggggtata taaaaaagac gtacgaccct taagtagtac agccacttct
     3121 caggcgttag gccctgggag tcgttatgag attgatgcca cgattgctga tatttattta
     3181 gtggatcatc atgatcgcca aaaaatcata ggaagaccaa cgctttacat tgtgattgat
     3241 gtgtttagtc ggatgatcac gggcttttat atcggctttg aaaatccgtc ttatgtggtg
     3301 gcgatgcagg cttttgtaaa tgcttgctct gacaaaacgg ccatttgtgc ccagcatgat
     3361 attgagatta gtagctcaga ctggccgtgt gtaggtttgc cagatgtgtt gctagcggac
     3421 cgtggcgaat taatgagtca tcaggtcgaa gccttagttt ctagttttaa tgtgcgagtg
     3481 gaaagtgctc cacctagacg tggcgatgct aaaggcatag tggaaagcac ttttagaaca
     3541 ctacaagccg agtttaagtc ctttgcacct ggcattgtag agggcagtcg gatcaaaagc
     3601 catggtgaaa cagactatag gttagatgca tctctgtcgg tatttgagtt cacacaaatt
     3661 attttgcgta cgatcttatt cagaaataac catctggtga tggataaata cgatcgagat
     3721 gctgattttc ctacagattt accgtctatt cctgtccagc tatggcaatg gggtatgcag
     3781 catcgtacag gtagtttaag ggctgtggag caagagcagt tgcgagtagc gttactgcct
     3841 cgccgaaagg tctctatttc ttcatttggc gttaatttgt ggggtttgta ttactcgggg
     3901 tcagagattc tgcgtgaggg ttggttgcag cggagcactg atatagctag acctcaacat
     3961 ttagaagcgg cttatgaccc agtgctggtt gatacgattt atttgtttcc gcaagttggc
     4021 agccgtgtat tttggcgctg taatctgacg gaacgtagtc ggcagtttaa aggtctctca
     4081 ttttgggagg tttgggatat acaagcacaa gaaaaacaca ataaagccaa tgcgaagcag
     4141 gatgagttaa ctaaacgcag ggagcttgag gcgtttattc agcaaaccat tcagaaagcg
     4201 aataagttaa cgcccagtac tactgagccc aaatcaacac gcattaagca gattaaaact
     4261 aataaaaaag aagccgtgac ctcggagcgt aaaaaacgtg cggagcattt gaagccaagc
     4321 tcttcaggtg atgaggctaa agttattcct ttcaacgcag tggaagcgga tgatcaagaa
     4381 gattacagcc tacccacata cgtgcctgaa ttatttcagg atccaccaga aaaggatgag
     4441 tcatgagtgc tacccggatt caagcagttt atcgtgatac gggggtagag gcttatcgtg
     4501 ataatccttt tatcgaggcc ttaccaccat tacaagagtc agtgaatagt gctgcatcac
     4561 tgaaatcctc tttacagctt acttcctctg acttgcaaaa gtcccgtgtt atcagagctc
     4621 ataccatttg tcgtattcca gatgactatt ttcagccatt aggtacgcat ttgctactaa
     4681 gtgagcgtat ttcggtcatg attcgaggtg gctacgtagg cagaaatcct aaaacaggag
     4741 atttacaaaa gcatttacaa aatggttatg agcgtgttca aacgggagag ttggagacat
     4801 ttcgctttga ggaggcacga tctacggcac aaagcttatt gttaattggt tgttctggta
     4861 gtgggaagac gacctctctt catcgtattc tagccacgta tcctcaggtg atttaccatc
     4921 gtgaactcaa tgtagagcag gtggtgtatt tgaaaataga ctgctcgcat aatggttcgc
     4981 taaaagaaat ctgcttgaat tttttcagag cgttggatcg agccttgggc tcgaactatg
     5041 agcgtcgtta tggcttaaaa cgtcatggta tagaaaccat gttggctttg atgtcgcaaa
     5101 tagccaatgc acatgcttta gggttgttgg ttattgatga aattcagcat ttaagccgct
     5161 ctcgttcggg tggatctcaa gagatgctga acttttttgt gacgatggtg aatattattg
     5221 gcgtaccagt gatgttgatt ggtaccccta aagcacgaga gatttttgag gctgatttgc
     5281 ggtctgcacg tagaggggca gggtttggag ctatattctg ggatcctata caacaaacgc
     5341 aacgtggaaa gcccaatcaa gagtggatcg cttttacgga taatctttgg caattacagc
     5401 ttttacaacg caaagatgcg ctgttatcgg atgaggtccg tgatgtgtgg tatgagctaa
     5461 gccaaggagt gatggacatt gtagtaaaac tttttgtact cgctcagctc cgtgcgctag
     5521 ctttaggcaa tgagcgtatt accgctggtt tattgcggca agtgtatcaa gatgagttaa
     5581 agcctgtgca ccccatgcta gaggcattac gctcgggtat cccagaacgc attgctcgtt
     5641 attctgatct agtcgttccc gagattgata aacggttaat ccaacttcag ctagatatcg
     5701 cagcgataca agaacaaaca ccagaagaaa aagcccttca agagttagat accgaagatc
     5761 agcgtcattt atatctgatg ctgaaagagg attacgattc aagcctgtta attcccacta
     5821 ttaaaaaagc gtttagccag aatccaacga tgacaagaca aaagttactg cctcttgttt
     5881 tgcagtggtt gatggaaggc gaaacggtag tgtcagaact agaaaagccc tccaagagta
     5941 aaaaggtttc ggctataaag gtagtcaagc ccagcgactg ggatagcttg cctgatacgg
     6001 atttacgtta tatctattca caacgccaac ctgaaaaaac catgcatgaa cggttaaaag
     6061 ggaaaggggt aatagtggat atggcgagct tatttaaaca agcaggttag ccatgagaaa
     6121 ctttcctgtt ccgtactcga atgagctgat ttatagcact attgcacggg caggcgttta
     6181 tcaagggatt gttagtccta agcagctgtt ggatgaggtg tatggcaacc gcaaggtggt
     6241 cgctacctta ggtctgccct cgcatttagg tgtgatagca agacatctac atcaaacagg
     6301 acgttacgct gttcagcagc ttatttatga gcatacctta ttccctttat atgctccgtt
     6361 tgtaggcaag gagcgccgag acgaagctat tcggttaatg gagtaccaag cgcaaggtgc
     6421 ggtgcattta atgctaggag tcgctgcttc tagagttaag agcgataacc gctttagata
     6481 ctgccctgat tgcgttgctc ttcagctaaa taggtatggg gaagcctttt ggcaacgaga
     6541 ttggtatttg cccgctttgc catattgtcc aaaacacggt gctttagtct tctttgatag
     6601 agctgtagat gatcaccgac atcaattttg ggctttgggt catactgagc tgctttcaga
     6661 ctaccccaaa gactccctat ctcaattaac agcactagct gcttatatag cccctctgtt
     6721 agatgctcca cgagcgcaag agctttcccc aagccttgag cagtggacgc tgttttatca
     6781 gcgcttagcg caggatctag ggctaaccaa aagcaagcac attcgtcatg acttggtggc
     6841 ggagagagtg aggcagactt ttagtgatga ggcactagag aaactggatt taaagttggc
     6901 agagaacaag gacacgtgtt ggctgaaaag tatattccgt aagcatagaa aagcctttag
     6961 ttatttacag catagtattg tgtggcaagc cttattgcca aaactaacgg ttatagaagc
     7021 gctacagcag gcaagtgctc ttactgagca ctctataacg acaagacctg ttagccagtc
     7081 tgtgcaacct aactctgaag atttatctgt taagcataaa gactggcagc aactagtgca
     7141 taaataccaa ggaattaagg cggcaagaca gtctttagag ggtggggtgc tatacgcttg
     7201 gctttaccga catgacaggg attggctagt tcactggaat caacagcatc aacaagagcg
     7261 tctggcaccc gcccctagag ttgattggaa ccaaagagat cgaattgctg tacgacaact
     7321 attaagaatc ataaagcgtc tagatagtag ccttgatcac ccaagagcga catcgagctg
     7381 gctgttaaag caaactccta acggaacctc tcttgcaaaa aatctacaga aactgccttt
     7441 ggtagcgctt tgcttaaagc gttactcaga gagtgtggaa gattatcaaa ttagacggat
     7501 tagccaagct tttattaagc ttaaacagga agatgttgag cttaggcgct ggcgattatt
     7561 aagaagtgca acgttatcta aagagcggat aactgaggaa gcacaaagat tcttggaaat
     7621 ggtttatggg gaagagtgag tggttaggct agctacattt aatgacaatg tgcaggttgt
     7681 acatattggt catttattcc gtaactcggg tcataaggag tggcgtattt ttgtttggtt
     7741 taatccaatg caagaacgga aatggactcg atttactcat ttgcctttat taagtcgagc
     7801 taaggtggtt aacagtacaa caaagcaaat aaataaggcg gatcgtgtga ttgagtttga
     7861 agcatcggat cttcaacgag ccaaaataat cgattttcct aatctctcgt cctttgcttc
     7921 cgtacgcaac aaggatggag cgcagagttc atttatttac gaagctgaaa caccatatag
     7981 caagactcgt tatcacatcc cacagttaga gctagctcgg tcattatttt taggggatcc
     8041 tctagagtcg acctgcaggc atgcaagctt ggctgttttg gcggatgaga gaagattttc
     8101 agcctgatac agattaaatc agaacgcaga agcggtctga taaaacagaa tttgcctggc
     8161 ggcagtagcg cggtggtccc acctgacccc atgccgaact cagaagtgaa acgccgtagc
     8221 gccgatggta gtgtggggtc tccccatgcg agagtaggga actgccaggc atcaaataaa
     8281 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
     8341 tctcctgagt aggacaaatc cgccgggagc ggatttgaac gttgcgaagc aacggcccgg
     8401 agggtggcgg gcaggacgcc cgccataaac tgccaggcat caaattaagc agaaggccat
     8461 cctgacggat ggcctttttg cgtttctaca aactcttttg tttatttttc taaatacatt
     8521 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     8581 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     8641 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     8701 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     8761 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     8821 tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     8881 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     8941 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     9001 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     9061 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     9121 ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     9181 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     9241 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     9301 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     9361 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     9421 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     9481 agattgattt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     9541 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     9601 tttctcgcca cgttcgccgc cggccagcct cgcagagcag gattcccgtt gagcaccgcc
     9661 aggtgcgaat aagggacagt gaagaaggaa cacccgctcg cgggtgggcc tacttcacct
     9721 atcctgcccg gcggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac
     9781 atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg
     9841 ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca
     9901 ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag
     9961 gagtcgcata agggagagcg tcgatccccg acagtaagac gggtaagcct gttgatgata
    10021 ccgctgcctt actgggtgca ttagccagtc tgaatgacct gtcacgggat aatccgaagt
    10081 ggtcagactg gaaaatcaga gggcaggaac tgctgaacag caaaaagtca gatagcacca
    10141 catagcagac ccgccataaa acgccctgag aagcccgtga cgggcttttc ttgtattatg
    10201 ggtagtttcc ttgcatgaat ccataaaagg cgcctgtagt gccatttacc cccattcact
    10261 gccagagccg tgagcgcagc gaactgaatg tcacgaaaaa gacagcgact caggtgcctg
    10321 atggtcggag acaaaaggaa tattcagcga tttgcccgag cttgcgaggg tgctacttaa
    10381 gcctttaggg ttttaaggtc tgttttgtag aggagcaaac agcgtttgcg acatcctttt
    10441 gtaatactgc ggaactgact aaagtagtga gttatacaca gggctgggat ctattctttt
    10501 tatctttttt tattctttct ttattctata aattataacc acttgaatat aaacaaaaaa
    10561 aacacacaaa ggtctagcgg aatttacaga gggtctagca gaatttacaa gttttccagc
    10621 aaaggtctag cagaatttac agatacccac aactcaaagg aaaaggacta gtaattatca
    10681 ttgactagcc catctcaatt ggtatagtga ttaaaatcac ctagaccaat tgagatgtat
    10741 gtctgaatta gttgttttca aagcaaatga actagcgatt agtcgctatg acttaacgga
    10801 gcatgaaacc aagctaattt tatgctgtgt ggcactactc aaccccacga ttgaaaaccc
    10861 tacaaggaaa gaacggacgg tatcgttcac ttataaccaa tacgttcaga tgatgaacat
    10921 cagtagggaa aatgcttatg gtgtattagc taaagcaacc agagagctga tgacgagaac
    10981 tgtggaaatc aggaatcctt tggttaaagg ctttgagatt ttccagtgga caaactatgc
    11041 caagttctca agcgaaaaat tagaattagt ttttagtgaa gagatattgc cttatctttt
    11101 ccagttaaaa aaattcataa aatataatct ggaacatgtt aagtcttttg aaaacaaata
    11161 ctctatgagg atttatgagt ggttattaaa agaactaaca caaaagaaaa ctcacaaggc
    11221 aaatatagag attagccttg atgaatttaa gttcatgtta atgcttgaaa ataactacca
    11281 tgagtttaaa aggcttaacc aatgggtttt gaaaccaata agtaaagatt taaacactta
    11341 cagcaatatg aaattggtgg ttgataagcg aggccgcccg actgatacgt tgattttcca
    11401 agttgaacta gatagacaaa tggatctcgt aaccgaactt gagaacaacc agataaaaat
    11461 gaatggtgac aaaataccaa caaccattac atcagattcc tacctacata acggactaag
    11521 aaaaacacta cacgatgctt taactgcaaa aattcagctc accagttttg aggcaaaatt
    11581 tttgagtgac atgcaaagta agtatgatct caatggttcg ttctcatggc tcacgcaaaa
    11641 acaacgaacc acactagaga acatactggc taaatacgga aggatctgag gttcttatgg
    11701 ctcttgtatc tatcagtgaa gcatcaagac taacaaacaa aagtagaaca actgttcacc
    11761 gttacatatc aaagggaaaa ctgtccatat gcacagatga aaacggtgta aaaaagatag
    11821 atacatcaga gcttttacga gtttttggtg catttaaagc tgttcaccat gaacagatcg
    11881 acaatgtaac agatgaacag catgtaacac ctaatagaac aggtgaaacc agtaaaacaa
    11941 agcaactaga acatgaaatt gaacacctga gacaacttgt tacagctcaa cagtcacaca
    12001 tagacagcct gaaacaggcg atgctgctta tcgaatcaaa gctgccgaca acacgggagc
    12061 cagtgacgcc tcccgtgggg aaaaaatcat ggcaattctg gaagaaatag cgctttcagc
    12121 ctgtgggcgg acaaaatagt tgggaactgg gaggggtgga aatggagttt ttaaggatta
    12181 tttagggaag agtgacaaaa tagatgggaa ctgggtgtag cgtcgtaagc taatacgaaa
    12241 attaaaaatg acaaaatagt ttggaactag atttcactta tctggttggt cgacactagt
    12301 attaccctgt tatccctaga tttaaatgat atcggatcct agtaagccac gttttaatta
    12361 atcagatccc gggtcaatag cggccgccac tcgagcacct aggaggcgcg ccacggccgc
    12421 gtcgacccca cgcccctctt taatacgacg ggcaatttgc acttcagaaa atgaagagtt
    12481 tgctttagcc ataacaaaag tccagtatgc tttttcacag cataactgga ctgatttcag
    12541 tttacaact
//
