LOCUS       sequence-237736-       11417 bp    DNA     circular SYN 15-APR-2025
DEFINITION  inducible expression of hGfi1 in colorectal cancer cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11417)
  TITLE     Growth Factor Independent 1 is a tumor suppressor gene in colorectal
            cancer
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11417)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pINDUCER20-hGfi1
FEATURES             Location/Qualifiers
     source          1..11417
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2230..2248,2249..2271,2272..2290,2291..2313,2314..2332,
                     2333..2355,2356..2374,2375..2397,2398..2416,2417..2439,
                     2440..2458,2459..2481,2482..2500)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2533..2571
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2568..2592
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2701..2725
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3989..4013)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4066..4092
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        4178..5389
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5226..5245
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(5448..6065,6066..6071,6072..6107,6108..6110,6111..6146,
                     6147..6149,6150..6185,6186..6194)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    6247..6820
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6414..6431)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6641..6660
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6836..7630
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6836..7630
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6890..6909)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7500..7519
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    7807..8395
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7860..7880)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8278..8289)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8470..8703
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8822..8840)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8908..9012
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9013..9081,9082..9873)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9231..9250)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10044..10632
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10533..10552
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10786..10803
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11043..11064
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11079..11084,11085..11102,11103..11109)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11117..11133
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11122..11144
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11141..11157
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11141..11157
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11207..11226
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactaggatc
     2221 ctttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
     2281 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
     2341 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
     2401 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
     2461 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagctcgg
     2521 tacccgggtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg
     2581 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg
     2641 gaccgatcca gcctccgcgg ccccgaacta gtccagtgtg gtggaattct gcagatatca
     2701 acaagtttgt acaaaaaagt tggcatgctg cgctcatttc tcgtcaaaag caagaaggct
     2761 cacagctacc accagccgcg ctccccagga ccagactatt ccctccgttt agagaatgta
     2821 ccggcgccta gccgagcaga cagcacttca aatgcaggcg gggcgaaggc ggagccccgg
     2881 gaccgtttgt cccccgaatc gcagctgacc gaagccccag acagagcctc cgcatcccca
     2941 gacagctgcg aaggcagcgt ctgcgaacgg agctcggagt ttgaggactt ctggaggccc
     3001 ccgtcaccct ccgcgtctcc agcctcggag aagtcaatgt gcccatcgct ggacgaagcc
     3061 cagcccttcc ccctgccttt caaaccgtac tcatggagcg gcctggcggg ttctgacctg
     3121 cggcacctgg tgcagagcta ccgaccgtgt ggggccctgg agcgtggcgc tggcctgggc
     3181 ctcttctgtg aacccgcccc ggagcctggc cacccggccg cgctgtacgg cccgaagcgg
     3241 gctgccggcg gcgcgggggc cggggcgcca gggagctgca gcgcaggggc cggtgccacc
     3301 gctggccctg gcctagggct ctacggcgac ttcgggtctg cggcagccgg gctgtatgag
     3361 aggcccacgg cagcggcggg cttgctgtac cccgagcgtg gccacgggct gcacgcagac
     3421 aagggcgctg gcgtcaaggt ggagtcggag ctgctgtgca cccgcctgct gctgggcggc
     3481 ggctcctaca agtgcatcaa gtgcagcaag gtgttctcca cgccgcacgg gctcgaggtg
     3541 cacgtgcgca ggtcccacag cggtaccaga ccctttgcct gcgagatgtg cggcaagacc
     3601 ttcgggcacg cggtgagcct ggagcagcac aaagccgtgc actcgcagga acggagcttt
     3661 gactgtaaga tctgtgggaa gagcttcaag aggtcatcca cactgtccac acacctgctt
     3721 atccactcag acactcggcc ctacccctgt cagtactgtg gcaagaggtt ccaccagaag
     3781 tcagacatga agaaacacac tttcatccac actggtgaga agcctcacaa gtgccaggtg
     3841 tgcggcaagg cattcagcca gagctccaac ctcatcaccc acagccgcaa acacacaggc
     3901 ttcaagccct tcggctgcga cctctgtggg aagggtttcc agaggaaggt ggacctccga
     3961 aggcaccggg agacgcagca tgggctcaaa ccaactttct tgtacaaagt ggttgatatc
     4021 cagcacagtg gcggccgctc gagtctagag ggcccgcggt tcgaataccc atacgacgtc
     4081 ccagactacg cttagtaatg attaattaaa ctagaaattc taccgggtag gggaggcgct
     4141 tttcccaagg cagtctggag taacccaccc aagatctggc ctccgcgccg ggttttggcg
     4201 cctcccgcgg gcgcccccct cctcacggcg agcgctgcca cgtcagacga agggcgcagc
     4261 gagcgtcctg atccttccgc ccggacgctc aggacagcgg cccgctgctc ataagactcg
     4321 gccttagaac cccagtatca gcagaaggac attttaggac gggacttggg tgactctagg
     4381 gcactggttt tctttccaga gagcggaaca ggcgaggaaa agtagtccct tctcggcgat
     4441 tctgcggagg gatctccgtg gggcggtgaa cgccgatgat tatataagga cgcgccgggt
     4501 gtggcacagc tagttccgtc gcagccggga tttgggtcgc ggttcttgtt tgtggatcgc
     4561 tgtgatcgtc acttggtgag tagcgggctg ctgggctggc cggggctttc gtggccgccg
     4621 ggccgctcgg tgggacggaa gcgtgtggag agaccgccaa gggctgtagt ctgggtccgc
     4681 gagcaaggtt gccctgaact gggggttggg gggagcgcag caaaatggcg gctgttcccg
     4741 agtcttgaat ggaagacgct tgtgaggcgg gctgtgaggt cgttgaaaca aggtgggggg
     4801 catggtgggc ggcaagaacc caaggtcttg aggccttcgc taatgcggga aagctcttat
     4861 tcgggtgaga tgggctgggg caccatctgg ggaccctgac gtgaagtttg tcactgactg
     4921 gagaactcgg tttgtcgtct gttgcggggg cggcagttat ggcggtgccg ttgggcagtg
     4981 cacccgtacc tttgggagcg cgcgccctcg tcgtgtcgtg acgtcacccg ttctgttggc
     5041 ttataatgca gggtggggcc acctgccggt aggtgtgcgg taggcttttc tccgtcgcag
     5101 gacgcagggt tcgggcctag ggtaggctct cctgaatcga caggcgccgg acctctggtg
     5161 aggggaggga taagtgaggc gtcagtttct ttggtcggtt ttatgtacct atcttcttaa
     5221 gtagctgaag ctccggtttt gaactatgcg ctcggggttg gcgagtgtgt tttgtgaagt
     5281 tttttaggca ccttttgaaa tgtaatcatt tgggtcaata tgtaattttc agtgttagac
     5341 tagtaaattg tccgctaaat tctggccgtt tttggctttt ttgttagacg aagcttggta
     5401 ccgagctcgg atctccaccc cgtaccggtc ctgcagtcga attcaccatg tctagactgg
     5461 acaagagcaa agtcataaac ggagctctgg aattactcaa tggtgtcggt atcgaaggcc
     5521 tgacgacaag gaaactcgct caaaagctgg gagttgagca gcctaccctg tactggcacg
     5581 tgaagaacaa gcgggccctg ctcgatgccc tgccaatcga gatgctggac aggcatcata
     5641 cccacttctg ccccctggaa ggcgagtcat ggcaagactt tctgcggaac aacgccaagt
     5701 cataccgctg tgctctcctc tcacatcgcg acggggctaa agtgcatctc ggcacccgcc
     5761 caacagagaa acagtacgaa accctggaaa atcagctcgc gttcctgtgt cagcaaggct
     5821 tctccctgga gaacgcactg tacgctctgt ccgccgtggg ccactttaca ctgggctgcg
     5881 tattggagga acaggagcat caagtagcaa aagaggaaag agagacacct accaccgatt
     5941 ctatgccccc acttctgaga caagcaattg agctgttcga ccggcaggga gccgaacctg
     6001 ccttcctttt cggcctggaa ctaatcatat gtggcctgga gaaacagcta aagtgcgaaa
     6061 gcggcgggcc gaccgacgcc cttgacgatt ttgacttaga catgctccca gccgatgccc
     6121 ttgacgactt tgaccttgat atgctgcctg ctgacgctct tgacgatttt gaccttgaca
     6181 tgctccccgg gtaactaagt aaggatccgc ggccgcacta gaggaattcc gcccctctcc
     6241 cccccccccc tctccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg
     6301 ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag
     6361 ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc
     6421 caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg
     6481 aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag
     6541 gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca
     6601 gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt
     6661 caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc
     6721 tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa
     6781 ccacggggac gtggttttcc tttgaaaaac acgatgataa gcttaccggt ccaccatgat
     6841 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     6901 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     6961 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga
     7021 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     7081 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     7141 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     7201 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     7261 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     7321 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga
     7381 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     7441 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     7501 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     7561 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     7621 gttcttctga tgtacaagta aagcggccgc gactctagat cataatcagc cataccacat
     7681 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
     7741 aaatgaatgc aattgttgtt gtttagtccc tcccaattcg atatcaagct tatcgcgata
     7801 gatcctaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     7861 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     7921 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     7981 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     8041 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     8101 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     8161 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     8221 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     8281 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     8341 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgag
     8401 atcctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     8461 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
     8521 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     8581 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     8641 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     8701 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     8761 cagagagtga gaggcccggg ttaattaagg aaagggctag atcattcttg aagacgaaag
     8821 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     8881 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     8941 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     9001 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     9061 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     9121 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     9181 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     9241 gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     9301 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     9361 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     9421 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     9481 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     9541 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     9601 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     9661 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     9721 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     9781 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     9841 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     9901 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     9961 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
    10021 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
    10081 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
    10141 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
    10201 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
    10261 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
    10321 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
    10381 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
    10441 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
    10501 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
    10561 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
    10621 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
    10681 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
    10741 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
    10801 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
    10861 taatgcagca agctcatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
    10921 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
    10981 aaaagctccc cgtggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
    11041 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
    11101 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgacatg
    11161 attacgaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
    11221 ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat
    11281 cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc
    11341 taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt
    11401 actacaaact tagtagt
//
