LOCUS       sequence-237858-         842 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Level 0 Golden-Gate compatible vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Petit-Houdenot Y, Langner T, Harant A, Win J, Kamoun S
  TITLE     A Clone Resource of Magnaporthe oryzae Effectors That Share Sequence
            and Structural Similarities Across Host-Specific Lineages.
  JOURNAL   Mol Plant Microbe Interact. 2020 Aug;33(8):1032-1035. doi: 
            10.1094/MPMI-03-20-0052-A. Epub 2020 Jun 22.
  PUBMED    32460610
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1094/MPMI-03-20-0052-A"; journalName: "Mol Plant Microbe 
            Interact"; date: "2020-08"; volume: "33"; issue: "8"; pages: 
            "1032-1035"
COMMENT     Sequence Label: pUC57-Kan_BR32_00066181
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(352..368)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(352..368)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(365..387)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    376..392
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(400..406,407..424,425..430))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    445..466
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(583..600)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cttggtctct aatgggttgg ccattgactg ctgattgtaa tatttatcca aaaattggtg
       61 ataaatctgt tggttggggt tctggtttta tgggttctgt tgctcatgtt agaattgaaa
      121 aagttgatta tgctgttgct ttgtggaaaa gagtttgtaa agctgttaaa ttggaagaat
      181 tgccaccagg tgttgaattg tttgctgaac caaatacttt gagaagaaat ccacaaccag
      241 ttcaaagaag aggtttggtt ggtagagaac cattgggtgg tttggaattg caagaatgag
      301 cttagagacc aagttccatc ccaatggcgc gccgagcttg gctcgagcat ggtcatagct
      361 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
      421 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
      481 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
      541 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
      601 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
      661 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
      721 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
      781 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
      841 cc
//
