LOCUS       sequence-237900-        8457 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Produces equimolar expression of mKokappa and mTq2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8457)
  AUTHORS   Botman D, de Groot DH, Schmidt P, Goedhart J, Teusink B
  TITLE     In vivo characterisation of fluorescent proteins in budding yeast.
  JOURNAL   Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.1038/s41598-019-38913-z.
  PUBMED    30783202
REFERENCE   2  (bases 1 to 8457)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2019-02-19"; volume: "9(1):2234. doi"; pages: " 
            10.1038/s41598-019-38913-z"
COMMENT     Sequence Label: pDRF1-GW mkok-T2A-mTurquoise2
FEATURES             Location/Qualifiers
     source          1..8457
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    168..192
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      209..218
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             236..883
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="SVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRVTM
                     AEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSASV
                     SAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMYLK
                     LEGGGNHKCQFKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
     CDS             911..964
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      980..989
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(986..988,989..991,992..1705)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1031..1052)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1292..1311)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1639..1660
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(1715..1739)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(1801..1820)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1802..1820)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      1968..2155
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2023..2047)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(2167..2184)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2167..2183)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2176..2198)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2619..3965
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(4076..4879)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(4880..5095)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(5120..5138)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5206..5310
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5311..5379,5380..6171)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5529..5548)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6342..6930
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6831..6850
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7084..7101
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7218..7239
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7254..7259,7260..7277,7278..7284)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7292..7308
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7297..7319
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7316..7332
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7316..7332
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(7362..7817)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7499..7520)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7711..7730
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tctacaaaaa cttttttttt ctatcaacct cgttgataaa ttttttcttt aacaatcgtt
       61 aataattaat taattggaaa ataaccattt tttctctctt ttatacacac attcaaaaga
      121 aagaaaaaaa atatacccca gcctcgacta gtgaattcgc ggccatcaca agtttgtaca
      181 aaaaagcagg ctgctagcgc taccggtcgc caccatggtg agcaagggcg aggagagtgt
      241 gattaaacca gagatgaaga tgaggtacta catggacggc tccgtcaatg ggcatgagtt
      301 cacaattgaa ggtgaaggca caggcagacc ttacgaggga catcaagaga tgacactacg
      361 cgtcacaatg gccgagggcg ggccaatgcc tttcgcgttt gacttagtgt cacacgtgtt
      421 ctgttacggc cacagagtat ttactaaata tccagaagag ataccagact atttcaaaca
      481 agcatttcct gaaggcctgt catgggaaag gtcgttggag ttcgaagatg gtgggtccgc
      541 ttcagtcagt gcgcatataa gccttagagg aaacaccttc taccacaaat ccaaatttac
      601 tggggttaac tttcctgccg atggtcctat catgcaaaac caaagtgttg attgggagcc
      661 atcaaccgag aaaattactg ccagcgacgg agttctgaag ggtgatgtta cgatgtacct
      721 aaaacttgaa ggaggcggca atcacaaatg ccaattcaag actacttaca aggcggcaaa
      781 agagattctt gaaatgccag gagaccatta catcggccat cgcctcgtca ggaaaaccga
      841 aggcaacatt actgagcagg tagaagatgc agtagctcat tccactggcg acgagctgta
      901 caagtccgga gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcccgg
      961 ccctggggat ccaccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt
     1021 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     1081 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     1141 caagctgccc gtgccctggc ccaccctcgt gaccaccctg tcctggggcg tgcagtgctt
     1201 cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     1261 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     1321 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     1381 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tactttagcg acaacgtcta
     1441 tatcaccgcc gacaagcaga agaacggcat caaggccaac ttcaagatcc gccacaacat
     1501 cgaggacggc ggcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     1561 ccccgtgctg ctgcccgaca accactacct gagcacccag tccaagctga gcaaagaccc
     1621 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     1681 cggcatggac gagctgtaca agtaaagcgg ccgcacccag ctttcttgta caaagtggtg
     1741 tagttaattc atgatggccg ctgcaggtcg acctcgaggg ggggcccggt acccaattcg
     1801 ccctatagtg agtcgtatta cgcgcggatc cagctttgga cttcttcgcc agaggtttgg
     1861 tcaagtctcc aatcaaggtt gtcggcttgt ctaccttgcc agaaatttac gaaaagatgg
     1921 aaaagggtca aatcgttggt agatacgttg ttgacacttc taaataagcg aatttcttat
     1981 gatttatgat ttttattatt aaataagtta taaaaaaaat aagtgtatac aaattttaaa
     2041 gtgactctta ggttttaaaa cgaaaattct tattcttgag taactctttc ctgtaggtca
     2101 ggttgctttc tcaggtatag catgaggtcg ctcttattga ccacacctct accggcatgc
     2161 caattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     2221 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     2281 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt
     2341 tctccttacg catctgtgcg gtatttcaca ccgcataatc ggatcgtact tgttacccat
     2401 cattgaattt tgaacatccg aacctgggag ttttccctga aacagatagt atatttgaac
     2461 ctgtataata atatatagtc tagcgcttta cggaagacaa tgtatgtatt tcggttcctg
     2521 gagaaactat tgcatctatt gcataggtaa tcttgcacgt cgcatccccg gttcattttc
     2581 tgcgtttcca tcttgcactt caatagcata tctttgttaa cgaagcatct gtgcttcatt
     2641 ttgtagaaca aaaatgcaac gcgagagcgc taatttttca aacaaagaat ctgagctgca
     2701 tttttacaga acagaaatgc aacgcgaaag cgctatttta ccaacgaaga atctgtgctt
     2761 catttttgta aaacaaaaat gcaacgcgag agcgctaatt tttcaaacaa agaatctgag
     2821 ctgcattttt acagaacaga aatgcaacgc gagagcgcta ttttaccaac aaagaatcta
     2881 tacttctttt ttgttctaca aaaatgcatc ccgagagcgc tatttttcta acaaagcatc
     2941 ttagattact ttttttctcc tttgtgcgct ctataatgca gtctcttgat aactttttgc
     3001 actgtaggtc cgttaaggtt agaagaaggc tactttggtg tctattttct cttccataaa
     3061 aaaagcctga ctccacttcc cgcgtttact gattactagc gaagctgcgg gtgcattttt
     3121 tcaagataaa ggcatccccg attatattct ataccgatgt ggattgcgca tactttgtga
     3181 acagaaagtg atagcgttga tgattcttca ttggtcagaa aattatgaac ggtttcttct
     3241 attttgtctc tatatactac gtataggaaa tgtttacatt ttcgtattgt tttcgattca
     3301 ctctatgaat agttcttact acaatttttt tgtctaaaga gtaatactag agataaacat
     3361 aaaaaatgta gaggtcgagt ttagatgcaa gttcaaggag cgaaaggtgg atgggtaggt
     3421 tatataggga tatagcacag agatatatag caaagagata cttttgagca atgtttgtgg
     3481 aagcggtatt cgcaatattt tagtagctcg ttacagtccg gtgcgttttt ggttttttga
     3541 aagtgcgtct tcagagcgct tttggttttc aaaagcgctc tgaagttcct atactttcta
     3601 gctagagaat aggaacttcg gaataggaac ttcaaagcgt ttccgaaaac gagcgcttcc
     3661 gaaaatgcaa cgcgagctgc gcacatacag ctcactgttc acgtcgcacc tatatctgcg
     3721 tgttgcctgt atatatatat acatgagaag aacggcatag tgcgtgttta tgcttaaatg
     3781 cgtacttata tgcgtctatt tatgtaggat gaaaggtagt ctagtacctc ctgtgatatt
     3841 atcccattcc atgcggggta tcgtatgctt ccttcagcac taccctttag ctgttctata
     3901 tgctgccact cctcaattgg attagtctca tccttcaatg ctatcatttc ctttgatatt
     3961 ggatcgatcc gatgataagc tgtcaaacat gagaattggg taataactga tataattaaa
     4021 ttgaagctct aatttgtgag tttagtatac atgcatttac ttataataca gttttttagt
     4081 tttgctggcc gcatcttctc aaatatgctt cccagcctgc ttttctgtaa cgttcaccct
     4141 ctaccttagc atcccttccc tttgcaaata gtcctcttcc aacaataata atgtcagatc
     4201 ctgtagagac cacatcatcc acggttctat actgttgacc caatgcgtct cccttgtcat
     4261 ctaaacccac accgggtgtc ataatcaacc aatcgtaacc ttcatctctt ccacccatgt
     4321 ctctttgagc aataaagccg ataacaaaat ctttgtcgct cttcgcaatg tcaacagtac
     4381 ccttagtata ttctccagta gatagggagc ccttgcatga caattctgct aacatcaaaa
     4441 ggcctctagg ttcctttgtt acttcttctg ccgcctgctt caaaccgcta acaatacctg
     4501 ggcccaccac accgtgtgca ttcgtaatgt ctgcccattc tgctattctg tatacacccg
     4561 cagagtactg caatttgact gtattaccaa tgtcagcaaa ttttctgtct tcgaagagta
     4621 aaaaattgta cttggcggat aatgccttta gcggcttaac tgtgccctcc atggaaaaat
     4681 cagtcaagat atccacatgt gtttttagta aacaaatttt gggacctaat gcttcaacta
     4741 actccagtaa ttccttggtg gtacgaacat ccaatgaagc acacaagttt gtttgctttt
     4801 cgtgcatgat attaaatagc ttggcagcaa caggactagg atgagtagca gcacgttcct
     4861 tatatgtagc tttcgacatg atttatcttc gtttcctgca tgtttttgtt ctgtgcagtt
     4921 gggttaagaa tactgggcaa tttcatgttt cttcaacact acatatgcgt atatatacca
     4981 atctaagtct gtgctccttc cttcgttctt ccttctgttc ggagattacc gaatcaaaaa
     5041 aatttcaagg aaaccgaaat caaaaaaaag aataaaaaaa aaatgatgaa ttgaaaagct
     5101 aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     5161 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     5221 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     5281 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     5341 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     5401 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     5461 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     5521 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     5581 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     5641 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     5701 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     5761 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     5821 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     5881 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     5941 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     6001 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     6061 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     6121 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     6181 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     6241 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     6301 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     6361 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     6421 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     6481 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     6541 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     6601 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     6661 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     6721 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     6781 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     6841 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     6901 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     6961 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     7021 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     7081 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     7141 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     7201 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     7261 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     7321 aacagctatg accatgatta cgccaagctt accgcatcag gaaattgtaa gcgttaatat
     7381 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
     7441 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
     7501 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
     7561 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
     7621 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
     7681 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
     7741 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
     7801 gccgctacag ggcgcgtcca ttcgccaagc ttcctgaaac ggagaaacat aaacaggcat
     7861 tgctgggatc acccatacat cactctgttt tgcctgacct tttccggtaa tttgaaaaca
     7921 aacccggtct cgaagcggag atccggcgat aattaccgca gaaataaacc catacacgag
     7981 acgtagaacc agccgcacat ggccggagaa actcctgcga gaatttcgta aactcgcgcg
     8041 cattgcatct gtatttccta atgcggcact tccaggcctc gatcgagacc gtttatccat
     8101 tgcttttttg ttgtcttttt ccctcgttca cagaaagtct gaagaagcta tagtagaact
     8161 atgagctttt tttgtttctg ttttcctttt tttttttttt acctctgtgg aaattgttac
     8221 tctcacactc tttagttcgt ttgtttgttt tgtttattcc aattatgacc ggtgacgaaa
     8281 cgtggtcgat ggtgggtacc gcttatgctc ccctccatta gtttcgatta tataaaaagg
     8341 ccaaatattg tattattttc aaatgtccta tcattatcgt ctaacatcta atttctctta
     8401 aattttttct ctttctttcc tataacacca atagtgaaaa tctttttttc ttctata
//
