LOCUS       sequence-237961-        4290 bp    DNA     circular SYN 04-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4290)
  AUTHORS   Young JA, Sermwittayawong D, Kim HJ, Nandu S, An N, 
            Erdjument-Bromage H, Tempst P, Coscoy L, Winoto A
  TITLE     Fas-associated death domain (FADD) and the E3 ubiquitin-protein 
            ligase TRIM21 interact to negatively regulate virus-induced 
            interferon production.
  JOURNAL   J Biol Chem. 2011 Feb 25;286(8):6521-31. Epub 2010 Dec 23.
  PUBMED    21183682
REFERENCE   2  (bases 1 to 4290)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 4, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2011-02-25"; volume: "286(8)"; pages: "6521-31. Epub 
            2010 Dec 23"
COMMENT     Sequence Label: pCI-His-hUbi
FEATURES             Location/Qualifiers
     source          1..4290
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          91..223
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     206..225
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        268..286
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             295..312
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             313..537
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="QIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFA
                     GKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             313..537
                     /codon_start=1
                     /gene="Homo sapiens UBC"
                     /product="human ubiquitin C"
                     /label=ubiquitin
                     /translation="QIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFA
                     GKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     polyA_signal    586..707
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(597..616)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     651..670
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      888..1343
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(975..994)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1185..1206
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(1410..1429)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1529..1551
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1589..1607)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1675..1779
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1832..1900,1901..2692)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2050..2069)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2863..3451
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3352..3371
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        3662..4041
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4042..4245
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4196..4216
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4242..4266
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 tatcacagtt aaattgctaa cgcagtcagt gcttctgaca caacagtctc gaacttaagc
       61 tgcagaagtt ggtcgtgagg cactgggcag gtaagtatca aggttacaag acaggtttaa
      121 ggagaccaat agaaactggg cttgtcgaga cagagaagac tcttgcgttt ctgataggca
      181 cctattggtc ttactgacat ccactttgcc tttctctcca caggtgtcca ctcccagttc
      241 aattacagct cttaaggcta gagtacttaa tacgactcac tataggctag catgcaccac
      301 catcaccatc atcagatctt cgtgaagacc ctgactggta agaccatcac tctcgaagtg
      361 gagccgagtg acaccattga gaatgtcaag gcaaagatcc aagacaagga aggcatccct
      421 cctgaccagc agaggttgat ctttgctggg aaacagctgg aagatggacg caccctgtct
      481 gactacaaca tccagaaaga gtccaccctg cacctggtac tccgtctcag aggtgggtga
      541 ggtacctcta gagtcgaccc gggcggccgc ttcgagcaga catgataaga tacattgatg
      601 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
      661 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
      721 gcattcattt tatgtttcag gttcaggggg agatgtggga ggttttttaa agcaagtaaa
      781 acctctacaa atgtggtaaa atcgataagg atccgggctg gcgtaatagc gaagaggccc
      841 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggacg cgccctgtag
      901 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
      961 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     1021 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     1081 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     1141 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     1201 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     1261 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     1321 caaaatatta acgcttacaa tttcctgatg cggtattttc tccttacgca tctgtgcggt
     1381 atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     1441 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     1501 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     1561 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     1621 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     1681 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     1741 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagnacaat aaccctgata
     1801 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     1861 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     1921 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     1981 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     2041 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     2101 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     2161 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     2221 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     2281 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     2341 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     2401 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     2461 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     2521 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     2581 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     2641 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     2701 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     2761 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     2821 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     2881 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     2941 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     3001 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     3061 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     3121 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     3181 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     3241 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     3301 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     3361 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     3421 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     3481 cctggccttt tgctggcctt ttgctcacat ggctcgacag atcttcaata ttggccatta
     3541 gccatattat tcattggtta tatagcataa atcaatattg gctattggcc attgcatacg
     3601 ttgtatctat atcataatat gtacatttat attggctcat gtccaatatg accgccatgt
     3661 tggcattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc
     3721 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     3781 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     3841 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     3901 caagtgtatc atatgccaag tccgccccct attgacgtca atgacggtaa atggcccgcc
     3961 tggcattatg cccagtacat gaccttacgg gactttccta cttggcagta catctacgta
     4021 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acaccaatgg gcgtggatag
     4081 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     4141 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaata accccgcccc gttgacgcaa
     4201 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgt
     4261 cagatcacta gaagctttat tgcggtagtt
//
