LOCUS       sequence-238031-        5657 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expresses Flag-tagged human CCDC11 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5657)
  TITLE     Expresses Flag-tagged human CCDC11 in mammalian cells
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5657)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Flag-hCCDC11
FEATURES             Location/Qualifiers
     source          1..5657
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..978
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
     promoter        1013..1031
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1013..1030
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             1069..1092
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    2687..2821
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2711..2730)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2765..2784
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2934..2954)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2934..2952)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2973..2989)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2973..2989)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2986..3008)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2997..3013
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3021..3027,3028..3045,3046..3051))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3066..3087
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3204..3221)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3375..3963)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3455..3474)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4134..4925,4926..4994))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4757..4776
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4995..5099)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5125..5580)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5262..5283)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5474..5493
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gccaattcaa tatggcgtat atggactcat gccaattcaa tatggtggat ctggacctgt
       61 gccaattcaa tatggcgtat atggactcgt gccaattcaa tatggtggat ctggacccca
      121 gccaattcaa tatggcggac ttggcaccat gccaattcaa tatggcggac ctggcactgt
      181 gccaactggg gaggggtcta cttggcacgg tgccaagttt gaggaggggt cttggccctg
      241 tgccaagtcc gccatattga attggcatgg tgccaataat ggcggccata ttggctatat
      301 gccaggatca atatataggc aatatccaat atggccctat gccaatatgg ctattggcca
      361 ggttcaatac tatgtattgg ccctatgcca tatagtattc catatatggg ttttcctatt
      421 gacgtagata gcccctccca atgggcggtc ccatatacca tatatggggc ttcctaatac
      481 cgcccatagc cactccccca ttgacgtcaa tggtctctat atatggtctt tcctattgac
      541 gtcatatggg cggtcctatt gacgtatatg gcgcctcccc cattgacgtc aattacggta
      601 aatggcccgc ctggctcaat gcccattgac gtcaatagga ccacccacca ttgacgtcaa
      661 tgggatggct cattgcccat tcatatccgt tctcacgccc cctattgacg tcaatgacgg
      721 taaatggccc acttggcagt acatcaatat ctattaatag taacttggca agtacattac
      781 tattggaagt acgccagggt acattggcag tactcccatt gacgtcaatg gcggtaaatg
      841 gcccgcgatg gctgccaagt acatccccat tgacgtcaat ggggaggggc aatgacgcaa
      901 atgggcgttc cattgacgta aatgggcggt aggcgtgcct aatgggaggt ctatataagc
      961 aatgctcgtt tagggaaccg ccattctgcc tggggacgtc ggagcaagct tgatttaggt
     1021 gacactatag aatacaagct acttgttctt tttgcaggat ccaccatgga ttacaaggat
     1081 gacgacgata agatctcgaa ttcaatgtac agccagcggt ttggcaccgt acagcgggag
     1141 gttaagggcc ccacccccaa agtggtgatc gtgagatcca agcctcctaa aggccaagga
     1201 gctgagcacc atctagaaag aatccgacgc agccatcaga agcataatgc tattttggct
     1261 tccattaagt caagtgagcg ggatcgcttg aaagctgagt gggaccagca caatgactgc
     1321 aagattttgg acagccttgt gcgagcaaga atcaaggatg ctgtgcaagg gtttatcatt
     1381 aacattgaag aaagacgaaa taagctacgt gagcttttag cattagaaga aaatgagtat
     1441 tttacagaaa tgcaattgaa gaaagaaacc attgaggaga aaaaagatag gatgagagag
     1501 aaaactaaat tactaaaaga gaagaatgaa aaagagaggc aggattttgt ggctgaaaag
     1561 ctagaccagc aattcaggga acgctgtgag gagctccgtg ttgaattgtt atctatccat
     1621 cagaagaagg tgtgtgagga gcggaaagca cagattgcat ttaatgagga gctgagcagg
     1681 caaaagctgg tggaagagca gatgttctcc aaactctggg aggaagaccg attagccaag
     1741 gaaaagcgag aagcccaaga ggcgaggaga cagaaagagc tgatggagaa cacacgcctg
     1801 gggctgaatg cccagatcac cagcatcaag gcacaaaggc aggcgacaca gctgctgaag
     1861 gaagaggagg cacgccttgt ggaaagtaac aacgcacaga ttaaacatga gaatgaacag
     1921 gatatgctaa agaaacagaa ggcaaagcag gaaactagga ccattttgca aaaagcccta
     1981 caagagagga tagaacatat tcagcaggaa tacagagacg aacaggactt gaacatgaag
     2041 ctcgtgcaaa gggcccttca agacttacag gaagaggcag ataaaaagaa acaaaaaaga
     2101 gaagatatga taagagaaca gaagatatac cataaatatt tggcacagag acgtgaggaa
     2161 gaaaaagctc aggagaaaga atttgacaga atattagagg aagacaaggc aaagaagttg
     2221 gctgagaagg acaaggagct gagacttgaa aaggaggcaa ggagacagct tgtggatgag
     2281 gtcatgtgta caagaaaact tcaagttcaa gaaaagttgc aacgagaagc taaagaacag
     2341 gaagaacgtg ctatggaaca gaaacacata aatgaaagtc ttaaagaact taactgtgaa
     2401 gagaaggaga attttgcaag acgccaacgt ttagcccagg agtacaggaa gcaacttcag
     2461 atgcaaatcg cctaccagca gcagtcccaa gaagcagaga aggaagagaa acgccgagag
     2521 tttgaagcag gtgtagcagc aaacaagatg tgtttggaca aggtccagga ggtcctgtcc
     2581 acccatcaag tgctgcctca aaacattcat cccatgcgca aggcatgccc cagtaagctt
     2641 ccaccgtagc tcgagcctct agaactatag tgagtcgtat tacgtagatc cagacatgat
     2701 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
     2761 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     2821 taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt
     2881 ttaattcgcg gccgcggcgc caatgcattg ggcccggtac ccagcttttg ttccctttag
     2941 tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     3001 tatccgctca caattccaca caacatacga gccgggagca taaagtgtaa agcctggggt
     3061 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     3121 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     3181 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     3241 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     3301 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     3361 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     3421 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     3481 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     3541 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     3601 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     3661 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     3721 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     3781 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     3841 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     3901 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     3961 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     4021 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     4081 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     4141 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     4201 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     4261 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     4321 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     4381 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     4441 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     4501 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     4561 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     4621 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     4681 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     4741 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     4801 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     4861 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     4921 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     4981 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     5041 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     5101 acatttcccc gaaaagtgcc acctaaattg taagcgttaa tattttgtta aaattcgcgt
     5161 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
     5221 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
     5281 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
     5341 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac
     5401 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
     5461 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
     5521 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
     5581 cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     5641 tattacgcca gtcgacc
//
