LOCUS       sequence-238038-        2164 bp    DNA     circular SYN 15-APR-2025
DEFINITION  MoClo golden gate assembly AB part for pL-lacO1 (LacI-repressed 
            promoter, as used in DOI:10.1038/35002125). Please see Supplemental 
            Documents for annotated Genbank file..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2164)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: P30m
FEATURES             Location/Qualifiers
     source          1..2164
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     11..29
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(33..76)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    79..100
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     protein_bind    145..161
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    186..206
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(212..229)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(383..971)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(463..482)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1142..1933,1934..2002))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1765..1784
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2003..2107)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aacctataaa aataggcgta tcacgaggca gaatttcaga taaaaaaaat ccttagcttt
       61 cgctaaggat gatttctgga attcgcggcc gcttctagag actagtgggt ctcaggagat
      121 aaatgtgagc ggataacatt gacattgtga gcggataaca agatactgag cacatactag
      181 agacctacta gtagcggccg ctgcaggctt cctcgctcac tgactcgctg cgctcggtcg
      241 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
      301 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
      361 aaaaggccgc gttgctggcg tttttccaca ggctccgccc ccctgacgag catcacaaaa
      421 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
      481 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
      541 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
      601 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
      661 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
      721 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
      781 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
      841 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
      901 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
      961 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     1021 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     1081 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     1141 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     1201 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     1261 ccagtgctgc aatgataccg cgcgacccac gctcaccggc tccagattta tcagcaataa
     1321 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     1381 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     1441 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     1501 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     1561 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     1621 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     1681 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     1741 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     1801 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     1861 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     1921 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     1981 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     2041 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     2101 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
     2161 catt
//
