LOCUS       sequence-238123-        9178 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Express M.EcoGII fused to LaminB1 with detabilization domain - 
            inducible.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9178)
  AUTHORS   Sobecki M, Souaid C, Boulay J, Guerineau V, Noordermeer D, Crabbe L
  TITLE     MadID, a Versatile Approach to Map Protein-DNA Interactions, 
            Highlights Telomere-Nuclear Envelope Contact Sites in Human Cells.
  JOURNAL   Cell Rep. 2018 Dec 4;25(10):2891-2903.e5. doi: 
            10.1016/j.celrep.2018.11.027.
  PUBMED    30517874
REFERENCE   2  (bases 1 to 9178)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2018.11"; journalName: "Cell Rep"; date: 
            "2018-12-4- 4"; volume: "25"; issue: "10"; pages: "2891-2903.e5"
COMMENT     Sequence Label: pRetroX-PTuner DD-linker-M.EcoGII-v5-Lamin B1
FEATURES             Location/Qualifiers
     source          1..9178
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    475..832
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     intron          895..1098
                     /label=EF-1-alpha intron A
                     /note="truncated intron upstream of the start codon of 
                     human EF-1-alpha"
     primer_bind     1055..1075
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     exon            1099..1128
                     /label=EF-1-alpha exon
                     /note="truncated exon 2 from human EF-1-alpha, beginning 
                     with a GT-to-CC mutation that reduces splicing efficiency"
     CDS             join(1188..1190,1191..1511)
                     /codon_start=1
                     /product="destabilization domain that can be stabilized by 
                     Shield1 in the ProteoTuner(TM) system"
                     /label=DD
                     /note="L106P mutant of FKBP12"
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKPE"
     CDS             2679..2720
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    join(4499..5073,5074..5076,5077..5085)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4666..4683)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4893..4912
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5105..5701
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5105..5124)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5601..5621
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5761..6354
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(6370..6386)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6370..6386)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6383..6405)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6394..6410
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6418..6424,6425..6442,6443..6448))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6463..6484
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6601..6618)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6772..7360)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6852..6871)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7531..8322,8323..8391))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8392..8496)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8564..8582
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8620..8642)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8742..8761
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8955..8977
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8969..8986
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8970..8986
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             join(9119..9178,1..410)
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
ORIGIN
        1 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
       61 ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag
      121 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg
      181 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgggctcaat
      241 aaaagagccc acaacccctc actcggcgcg ccagtcctcc gattgactga gtcgcccggg
      301 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggcct cgctgttcct
      361 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca tttgggggct
      421 cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg aggtaagctg
      481 gccagcaact tatctgtgtc tgtccgattg tctagtgtct atgactgatt ttatgcgcct
      541 gcgtcggtac tagttagcta actagctctg tatctggcgg acccgtggtg gaactgacga
      601 gttcggaaca cccggccgca accctgggag acgtcccagg gacttcgggg gccgtttttg
      661 tggcccgacc tgagtccaaa aatcccgatc gttttggact ctttggtgca ccccccttag
      721 aggagggata tgtggttctg gtaggagacg agaacctaaa acagttcccg cctccgtctg
      781 aatttttgct ttcggtttgg gaccgaagcc gcgccgcgcg tcttgtctgc tgcagcatcg
      841 ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc tgaaaatacg cgtgtcgagc
      901 ttttggagta cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac
      961 actgagtggg tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa
     1021 tttgcccttt ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt
     1081 ttttttcttc catttcagcc gtcgtgaaaa ctacccctaa aagccaaaaa aatcgaattc
     1141 ccaaacttaa gcttggtacc gagctcggat ctcacgtggg cccgcggatg ggagtgcagg
     1201 tggaaaccat ctccccagga gacgggcgca ccttccccaa gcgcggccag acctgtgtgg
     1261 tgcactacac cgggatgctt gaagatggaa agaaagtcga ttcctcccgg gacagaaaca
     1321 agccctttaa gtttatgcta ggcaagcagg aggtgatccg aggctgggaa gaaggggttg
     1381 cccagatgag tgtgggtcag agagccaaac tgactatatc tccagattat gcctatggtg
     1441 ccactgggca cccaggcatc atcccaccac atgccactct cgtcttcgat gtggagcttc
     1501 taaaaccgga accgcggccg cagatctatc gatggatcct atccggactc agatctcgag
     1561 ctcaagcttc gaattctgca gtcgacggta ccgcgggcag cgctgccaac ctgccgggga
     1621 tccgtatgct taatactgta aaaatatcca gttgtgagtt aatcaacgcc gactgcctgg
     1681 aatttatccg gtcgttaccc gaaaattctg ttgacctgat agtcacggac ccgccgtact
     1741 ttaaagtgaa gcccgagggc tgggataacc agtggaaggg cgacgatgat tacctgaagt
     1801 ggctggacca gtgtctggcg cagttctggc gggtgctgaa acctgccgga agtctttacc
     1861 tgttctgtgg ccatcgcctg gcatctgaca ttgaaatcat gatgcgtgaa cgcttcagtg
     1921 tgctgaacca tattatctgg gcaaagccgt ccggacgctg gaacgggtgc aacaaggaaa
     1981 gcctgcgggc gtatttcccc gccacagagc gcattctgtt cgcggaacat tatcaggggc
     2041 cgtatcgccc gaaagatgcc gggtatgagg cgaagggcag ggcactgaaa cagcatgtga
     2101 tggccccgct gattgcttac tttcgtgatg cgcgcgctgc cctggggata acggcaaaac
     2161 agattgcaga tgccacagga aagaaaaaca tggtgccgca ctggttcagt gccagtcagt
     2221 ggcagctacc gaacgaaagc gattatctga aattacagtc gctgtttgcc cgggtggcag
     2281 aagagaaaca tcagcgcggg gaactggaaa agccacacca ccagctggtc agcacataca
     2341 gtgagctgaa ccggaagtat atggaactgc tgagtgaata taaaaatttg cggcggtatt
     2401 tcggtgtgac ggtgcaggtg ccgtacaccg atgtgtggac gtataaaccg gtgcagtact
     2461 atccagggaa acatccgtgc gaaaaaccgg cagaaatgct gcagcagata atcagcgcaa
     2521 gtagtcgtcc tggtgatctg gttgcggatt ttttcatggg gtcgggttca acggtaaaag
     2581 cggcgatggc actggggcgt cgtgcgattg gtgttgagct ggagaccgga cgttttgagc
     2641 agacagtcag ggaagttcag gatttaatcg ttccatgggg taagcctatc cctaaccctc
     2701 tcctcggtct cgattctacg cgtaccggca tggcgactgc gacccccgtg ccgccgcgga
     2761 tgggcagccg cgctggcggc cccaccacgc cgctgagccc cacgcgcctg tcgcggctcc
     2821 aggagaagga ggagctgcgc gagctcaatg accggctggc ggtgtacatc gacaaggtgc
     2881 gcagcctgga gacggagaac agcgcgctgc agctgcaggt gacggagcgc gaggaggtgc
     2941 gcggccgtga gctcaccggc ctcaaggcgc tctacgagac cgagctggcc gacgcgcgac
     3001 gcgcgctcga cgacacggcc cgcgagcgcg ccaagctgca gatcgagctg ggcaagtgca
     3061 aggcggaaca cgaccagctg ctcctcaact atgctaagaa ggaatctgat cttaatggcg
     3121 cccagatcaa gcttcgagaa tatgaagcag cactgaattc gaaagatgca gctcttgcta
     3181 ctgcacttgg tgacaaaaaa agtttagagg gagatttgga ggatctgaag gatcagattg
     3241 cccagttgga agcctcctta gctgcagcca aaaaacagtt agcagatgaa actttactta
     3301 aagtagattt ggagaatcgt tgtcagagcc ttactgagga cttggagttt cgcaaaagca
     3361 tgtatgaaga ggagattaac gagaccagaa ggaagcatga aacgcgcttg gtagaggtgg
     3421 attctgggcg tcaaattgag tatgagtaca agctggcgca agcccttcat gagatgagag
     3481 agcaacatga tgcccaagtg aggctgtata aggaggagct ggagcagact taccatgcca
     3541 aacttgagaa tgccagactg tcatcagaga tgaatacttc tactgtcaac agtgccaggg
     3601 aagaactgat ggaaagccgc atgagaattg agagcctttc atcccagctt tctaatctac
     3661 agaaagagtc tagagcatgt ttggaaagga ttcaagaatt agaggacttg cttgctaaag
     3721 aaaaagacaa ctctcgtcgc atgctgacag acaaagagag agagatggcg gaaataaggg
     3781 atcaaatgca gcaacagctg aatgactatg aacagcttct tgatgtaaag ttagccctgg
     3841 acatggaaat cagtgcttac aggaaactct tagaaggcga agaagagagg ttgaagctgt
     3901 ctccaagccc ttcttcccgt gtgacagtat cccgagcatc ctcaagtcgt agtgtacgta
     3961 caactagagg aaagcggaag agggttgatg tggaagaatc agaggcgagt agtagtgtta
     4021 gcatctctca ttccgcctca gccactggaa atgtttgcat cgaagaaatt gatgttgatg
     4081 ggaaatttat ccgcttgaag aacacttctg aacaggatca accaatggga ggctgggaga
     4141 tgatcagaaa aattggagac acatcagtca gttataaata tacctcaaga tatgtgctga
     4201 aggcaggcca gactgttaca atttgggctg caaacgctgg tgtcacagcc agccccccaa
     4261 ctgacctcat ctggaagaac cagaactcgt ggggcactgg cgaagatgtg aaggttatat
     4321 tgaaaaattc tcagggagag gaggttgctc aaagaagtac agtctttaaa acaaccatac
     4381 ctgaagaaga ggaggaggag gaagaagcag ctggagtggt tgttgaggaa gaacttttcc
     4441 accagcaggg aaccccaaga gcatccaata gaagctgtgc aattatgtaa ccatggcgcc
     4501 cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg
     4561 cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga
     4621 aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa
     4681 tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa
     4741 caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct
     4801 gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg
     4861 ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg
     4921 ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca
     4981 catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg
     5041 acgtggtttt cctttgaaaa acacgatgat aagcttgcca caacccacaa ggagacgacc
     5101 ttccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tcccccgggc
     5161 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgaccc
     5221 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     5281 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
     5341 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     5401 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     5461 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     5521 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     5581 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     5641 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     5701 caccggtgcg gcttcgagga taaaataaaa gattttattt agtctccaga aaaagggggg
     5761 aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc
     5821 atggaaaaat acataactga gaatagagaa gttcagatca aggtcaggaa cagatggaac
     5881 agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc
     5941 aagaacagat ggaacagctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg
     6001 ccccggctca gggccaagaa cagatggtcc ccagatgcgg tccagccctc agcagtttct
     6061 agagaaccat cagatgtttc cagggtgccc caaggacctg aaatgaccct gtgccttatt
     6121 tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc cccgagctca
     6181 ataaaagagc ccacaacccc tcactcgggg cgccagtcct ccgattgact gagtcgcccg
     6241 ggtacccgtg tatccaataa accctcttgc agttgcatcc gacttgtggt ctcgctgttc
     6301 cttgggaggg tctcctctga gtgattgact acccgtcagc gggggtcttt cattgaattc
     6361 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     6421 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     6481 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     6541 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     6601 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     6661 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     6721 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     6781 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     6841 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     6901 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     6961 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     7021 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     7081 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     7141 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     7201 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     7261 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     7321 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     7381 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     7441 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     7501 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     7561 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     7621 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     7681 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     7741 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     7801 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     7861 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     7921 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     7981 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     8041 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     8101 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     8161 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     8221 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     8281 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     8341 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     8401 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     8461 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     8521 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
     8581 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
     8641 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
     8701 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt
     8761 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     8821 catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
     8881 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
     8941 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca agcttatgtg
     9001 aaagacccca cctgtaggtt tggcaagcta gcttaagtaa cgccattttg caaggcatgg
     9061 aaaaatacat aactgagaat agaaaagttc agatcaaggt caggaacaga tggaacagct
     9121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaa
//
