LOCUS       sequence-238136-         833 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Level 0 Golden-Gate compatible vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Petit-Houdenot Y, Langner T, Harant A, Win J, Kamoun S
  TITLE     A Clone Resource of Magnaporthe oryzae Effectors That Share Sequence
            and Structural Similarities Across Host-Specific Lineages.
  JOURNAL   Mol Plant Microbe Interact. 2020 Aug;33(8):1032-1035. doi: 
            10.1094/MPMI-03-20-0052-A. Epub 2020 Jun 22.
  PUBMED    32460610
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1094/MPMI-03-20-0052-A"; journalName: "Mol Plant Microbe 
            Interact"; date: "2020-08"; volume: "33"; issue: "8"; pages: 
            "1032-1035"
COMMENT     Sequence Label: pUC57-Kan_FR13_00108711
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(501..517)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(501..517)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(514..536)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    525..541
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(549..555,556..573,574..579))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    594..615
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(732..749)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ttggtctcta atgggtgatg aatctattgg ttctacttct ccaaggagac caggttctcc
       61 aactgatcca ggttctttgt tggctgctgg tggttctcca ggttctagat ctgctccagc
      121 tactgatggt gctttgccac caagagatca aggtatgatt ggttttggtc caattccatc
      181 tgctagaaat agaaaaaaat ttccacaacc aaatattgtt ccaggtccag atggtactgc
      241 tgctcatcca gaaggtccaa tgtgtattat tactttggtt agaccatttg gtagatctga
      301 atctttgcca ttgagagctg gtgttgaagg tattccagat ggttggtctt ggaaatggag
      361 aaaagctcca tatgctcaag ataaaaaaaa atctagagtt tttggtggtt tgggtagatt
      421 tggtagaact tctcaaagaa ctggttgagc ttagagacca agttccatcc caatggcgcg
      481 ccgagcttgg ctcgagcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
      541 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
      601 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
      661 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
      721 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
      781 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgc
//
