LOCUS       sequence-23842-e         826 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Guo XV, Monteleone M, Klotzsche M, Kamionka A, Hillen W, Braunstein 
            M, Ehrt S, Schnappinger D
  TITLE     Silencing Mycobacterium smegmatis by using tetracycline repressors.
  JOURNAL   J Bacteriol. 2007 Jul . 189(13):4614-23.
  PUBMED    17483222
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Bacteriol. 2007 Jul . 189(13):4614-23."
COMMENT     Sequence Label: pME1mL1
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(374..401)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(491..508)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggggggnngg cgtangnant nanangtana tctccttcgc atgcggatcg tgctcatttc
       61 gggcggcgaa tctctcggcg tcgaaatgat gtatgccgtg ctggtcgtac acacgggtac
      121 tcccagagcc tatctatcac tgatagggag gacaaactct atcactgata gggagttctc
      181 ccgctcgtca gagacccttc tcacaccggg cctgtgcgtg cgaacgcaca gatcaacgcc
      241 gggatggcgt agagctcact gcgcagcacc gtggggatgc ggccgatcat cacgtcgcgg
      301 atcgtgccgc ctcccacggc cgtgacggtg ccgacgatcc aatatttcta gagtttaaac
      361 agtattaaac gcaaaaaggc catccgtcag gatggccttc tgcttagcta atcaactagt
      421 tctagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
      481 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
      541 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
      601 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
      661 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcnacgcctc aagtcagang
      721 tggcgaaacc cgacaggact ataaagatac caggcngttt ccccgtgaaa actccctcgt
      781 gngctctctt gttcnaacct tgccgcttac cggaaacntn tcccct
//
