LOCUS       sequence-238770-        9649 bp    DNA     circular SYN 15-APR-2025
DEFINITION  LYS2-MET17 integrating (YIp) vector for stable genomic integration 
            in the BY4741 background..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9649)
  AUTHORS   Flagg MP, Kao A, Hampton RY
  TITLE     ICE plasmids: combining the ease of yeast recombination cloning with
            the stability of genomic integration.
  JOURNAL   Yeast. 2019 May 10. doi: 10.1002/yea.3400.
  PUBMED    31074531
REFERENCE   2  (bases 1 to 9649)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2019
            May 10. doi: 10.1002/yea.3400."
COMMENT     Sequence Label: YIp LYS2-MET17
FEATURES             Location/Qualifiers
     source          1..9649
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     120..139
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     373..390
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    507..528
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(543..548,549..566,567..573)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    581..597
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     586..608
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     605..621
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     605..621
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             617..997
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLSI
                     PSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWTRPVAAH"
     primer_bind     640..660
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        642..660
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    673..780
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     697..713
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     697..713
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(747..763)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(748..764)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(788..807)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(789..807)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(817..834)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(817..833)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(826..848)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      974..1429
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1061..1080)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1271..1292
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1486..1725
                     /gene="S. cerevisiae LYS2"
                     /label=LYS2 promoter
     CDS             1726..5904
                     /codon_start=1
                     /gene="S. cerevisiae LYS2"
                     /product="alpha-aminoadipate reductase, required for lysine
                     biosynthesis"
                     /label=LYS2
                     /note="yeast auxotrophic marker"
                     /translation="MTNEKVWIEKLDNPTLSVLPHDFLRPQQEPYTKQATYSLQLPQLD
                     VPHDSFSNKYAVALSVWAALIYRVTGDDDIVLYIANNKILRFNIQPTWSFNELYSTINN
                     ELNKLNSIEANFSFDELAEKIQSCQDLERTPQLFRLAFLENQDFKLDEFKHHLVDFALN
                     LDTSNNAHVLNLIYNSLLYSNERVTIVADQFTQYLTAALSDPSNCITKISLITASSKDS
                     LPDPTKNLGWCDFVGCIHDIFQDNAEAFPERTCVVETPTLNSDKSRSFTYRDINRTSNI
                     VAHYLIKTGIKRGDVVMIYSSRGVDLMVCVMGVLKAGATFSVIDPAYPPARQTIYLGVA
                     KPRGLIVIRAAGQLDQLVEDYINDELEIVSRINSIAIQENGTIEGGKLDNGEDVLAPYD
                     HYKDTRTGVVVGPDSNPTLSFTSGSEGIPKGVLGRHFSLAYYFNWMSKRFNLTENDKFT
                     MLSGIAHDPIQRDMFTPLFLGAQLYVPTQDDIGTPGRLAEWMSKYGCTVTHLTPAMGQL
                     LTAQATTPFPKLHHAFFVGDILTKRDCLRLQTLAENCRIVNMYGTTETQRAVSYFEVKS
                     KNDDPNFLKKLKDVMPAGKGMLNVQLLVVNRNDRTQICGIGEIGEIYVRAGGLAEGYRG
                     LPELNKEKFVNNWFVEKDHWNYLDKDNGEPWRQFWLGPRDRLYRTGDLGRYLPNGDCEC
                     CGRADDQVKIRGFRIELGEIDTHISQHPLVRENITLVRKNADNEPTLITFMVPRFDKPD
                     DLSKFQSDVPKEVETDPIVKGLIGYHLLSKDIRTFLKKRLASYAMPSLIVVMDKLPLNP
                     NGKVDKPKLQFPTPKQLNLVAENTVSETDDSQFTNVEREVRDLWLSILPTKPASVSPDD
                     SFFDLGGHSILATKMIFTLKKKLQVDLPLGTIFKYPTIKAFAAEIDRIKSSGGSSQGEV
                     VENVTANYAEDAKKLVETLPSSYPSREYFVEPNSAEGKTTINVFVTGVTGFLGSYILAD
                     LLGRSPKNYSFKVFAHVRAKDEEAAFARLQKAGITYGTWNEKFASNIKVVLGDLSKSQF
                     GLSDEKWMDLANTVDIIIHNGALVHWVYPYAKLRDPNVISTINVMSLAAVGKPKFFDFV
                     SSTSTLDTEYYFNLSDKLVSEGKPGILESDDLMNSASGLTGGYGQSKWAAEYIIRRAGE
                     RGLRGCIVRPGYVTGASANGSSNTDDFLLRFLKGSVQLGKIPDIENSVNMVPVDHVARV
                     VVATSLNPPKENELAVAQVTGHPRILFKDYLYTLHDYGYDVEIESYSKWKKSLEASVID
                     RNEENALYPLLHMVLDNLPESTKAPELDDRNAVASLKKDTAWTGVDWSNGIGVTPEEVG
                     IYIAFLNKVGFLPPPTHNDKLPLPSIELTQAQISLVASGAGARGSSAAA"
     primer_bind     complement(6305..6324)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6424..6446
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6484..6502)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             6759..8093
                     /codon_start=1
                     /gene="S. cerevisiae MET17"
                     /gene_synonym="MET15; MET25"
                     /product="O-acetylserine and O-acetylhomoserine 
                     sulfhydrylase, required for sulfur amino acid synthesis"
                     /label=MET17
                     /translation="MPSHFDTVQLHAGQENPGDNAHRSRAVPIYATTSYVFENSKHGSQ
                     LFGLEVPGYVYSRFQNPTSNVLEERIAALEGGAAALAVSSGQAAQTLAIQGLAHTGDNI
                     VSTSYLYGGTYNQFKISFKRFGIEARFVEGDNPEEFEKVFDERTKAVYLETIGNPKYNV
                     PDFEKIVAIAHKHGIPVVVDNTFGAGGYFCQPIKYGADIVTHSATKWIGGHGTTIGGII
                     VDSGKFPWKDYPEKFPQFSQPAEGYHGTIYNEAYGNLAYIVHVRTELLRDLGPLMNPFA
                     SFLLLQGVETLSLRAERHGENALKLAKWLEQSPYVSWVSYPGLASHSHHENAKKYLSNG
                     FGGVLSFGVKDLPNADKETDPFKLSGAQVVDNLKLASNLANVGDAKTLVIAPYFTTHKQ
                     LNDKEKLASGVTKDLIRVSVGIEFIDDIIADFQQSFETVFAGQKP"
     promoter        8144..8248
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8249..8317,8318..9109)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8467..8486)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9280..9649,1..219)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
       61 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
      121 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
      181 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
      241 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
      301 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
      361 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
      421 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
      481 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
      541 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
      601 cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg
      661 gaacaaaagc tggagctcca ccgcggtggc ggccgctcta gaactagtgg atcccccggg
      721 ctgcaggaat tcgatatcaa gcttatcgat accgtcgacc tcgagggggg gcccggtacc
      781 caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg
      841 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
      901 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
      961 gaatggcgaa tggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     1021 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     1081 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     1141 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     1201 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     1261 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     1321 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     1381 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc ctgatgcggt
     1441 attttctcct tacgcatctg tgcggtattt cacaccgcat agatcaattc cacttgcaat
     1501 tacataaaaa attccggcgg tttttcgcgt gtgactcaat gtcgaaatac ctgcctaatg
     1561 aacatgaaca tcgcccaaat gtatttgaag acccgctggg agaagttcaa gatatataag
     1621 taacaagcag ccaatagtat aaaaaaaaat ctgagtttat tacctttcct ggaatttcag
     1681 tgaaaaactg ctaattatag agagatatca cagagttact cactaatgac taacgaaaag
     1741 gtctggatag agaagttgga taatccaact ctttcagtgt taccacatga ctttttacgc
     1801 ccacaacaag aaccttatac gaaacaagct acatattcgt tacagctacc tcagctcgat
     1861 gtgcctcatg atagtttttc taacaaatac gctgtcgctt tgagtgtatg ggctgcattg
     1921 atatatagag taaccggtga cgatgatatt gttctttata ttgcgaataa caaaatctta
     1981 agattcaata ttcaaccaac gtggtcattt aatgagctgt attctacaat taacaatgag
     2041 ttgaacaagc tcaattctat tgaggccaat ttttcctttg acgagctagc tgaaaaaatt
     2101 caaagttgcc aagatctgga aaggacccct cagttgttcc gtttggcctt tttggaaaac
     2161 caagatttca aattagacga gttcaagcat catttagtgg actttgcttt gaatttggat
     2221 accagtaata atgcgcatgt tttgaactta atttataaca gcttactgta ttcgaatgaa
     2281 agagtaacca ttgttgcgga ccaatttact caatatttga ctgctgcgct aagcgatcca
     2341 tccaattgca taactaaaat ctctctgatc accgcatcat ccaaggatag tttacctgat
     2401 ccaactaaga acttgggctg gtgcgatttc gtggggtgta ttcacgacat tttccaggac
     2461 aatgctgaag ccttcccaga gagaacctgt gttgtggaga ctccaacact aaattccgac
     2521 aagtcccgtt ctttcactta tcgcgacatc aaccgcactt ctaacatagt tgcccattat
     2581 ttgattaaaa caggtatcaa aagaggtgat gtagtgatga tctattcttc taggggtgtg
     2641 gatttgatgg tatgtgtgat gggtgtcttg aaagccggcg caaccttttc agttatcgac
     2701 cctgcatatc ccccagccag acaaaccatt tacttaggtg ttgctaaacc acgtgggttg
     2761 attgttatta gagctgctgg acaattggat caactagtag aagattacat caatgatgaa
     2821 ttggagattg tttcaagaat caattccatc gctattcaag aaaatggtac cattgaaggt
     2881 ggcaaattgg acaatggcga ggatgttttg gctccatatg atcactacaa agacaccaga
     2941 acaggtgttg tagttggacc agattccaac ccaaccctat ctttcacatc tggttccgaa
     3001 ggtattccta agggtgttct tggtagacat ttttccttgg cttattattt caattggatg
     3061 tccaaaaggt tcaacttaac agaaaatgat aaattcacaa tgctgagcgg tattgcacat
     3121 gatccaattc aaagagatat gtttacacca ttatttttag gtgcccaatt gtatgtccct
     3181 actcaagatg atattggtac accgggccgt ttagcggaat ggatgagtaa gtatggttgc
     3241 acagttaccc atttaacacc tgccatgggt caattactta ctgcccaagc tactacacca
     3301 ttccctaagt tacatcatgc gttctttgtg ggtgacattt taacaaaacg tgattgtctg
     3361 aggttacaaa ccttggcaga aaattgccgt attgttaata tgtacggtac cactgaaaca
     3421 cagcgtgcag tttcttattt cgaagttaaa tcaaaaaatg acgatccaaa ctttttgaaa
     3481 aaattgaaag atgtcatgcc tgctggtaaa ggtatgttga acgttcagct actagttgtt
     3541 aacaggaacg atcgtactca aatatgtggt attggcgaaa taggtgagat ttatgttcgt
     3601 gcaggtggtt tggccgaagg ttatagagga ttaccagaat tgaataaaga aaaatttgtg
     3661 aacaactggt ttgttgaaaa agatcactgg aattatttgg ataaggataa tggtgaacct
     3721 tggagacaat tctggttagg tccaagagat agattgtaca gaacgggtga tttaggtcgt
     3781 tatctaccaa acggtgactg tgaatgttgc ggtagggctg atgatcaagt taaaattcgt
     3841 gggttcagaa tcgaattagg agaaatagat acgcacattt cccaacatcc attggtaaga
     3901 gaaaacatta ctttagttcg caaaaatgcc gacaatgagc caacattgat cacatttatg
     3961 gtcccaagat ttgacaagcc agatgacttg tctaagttcc aaagtgatgt tccaaaggag
     4021 gttgaaactg accctatagt taagggctta atcggttacc atcttttatc caaggacatc
     4081 aggactttct taaagaaaag attggctagc tatgctatgc cttccttgat tgtggttatg
     4141 gataaactac cattgaatcc aaatggtaaa gttgataagc ctaaacttca attcccaact
     4201 cccaagcaat taaatttggt agctgaaaat acagtttctg aaactgacga ctctcagttt
     4261 accaatgttg agcgcgaggt tagagactta tggttaagta tattacctac caagccagca
     4321 tctgtatcac cagatgattc gtttttcgat ttaggtggtc attctatctt ggctaccaaa
     4381 atgattttta ccttaaagaa aaagctgcaa gttgatttac cattgggcac aattttcaag
     4441 tatccaacga taaaggcctt tgccgcggaa attgacagaa ttaaatcatc gggtggatca
     4501 tctcaaggtg aggtcgtcga aaatgtcact gcaaattatg cggaagacgc caagaaattg
     4561 gttgagacgc taccaagttc gtacccctct cgagaatatt ttgttgaacc taatagtgcc
     4621 gaaggaaaaa caacaattaa tgtgtttgtt accggtgtca caggatttct gggctcctac
     4681 atccttgcag atttgttagg acgttctcca aagaactaca gtttcaaagt gtttgcccac
     4741 gtcagggcca aggatgaaga agctgcattt gcaagattac aaaaggcagg tatcacctat
     4801 ggtacttgga acgaaaaatt tgcctcaaat attaaagttg tattaggcga tttatctaaa
     4861 agccaatttg gtctttcaga tgagaagtgg atggatttgg caaacacagt tgatataatt
     4921 atccataatg gtgcgttagt tcactgggtt tatccatatg ccaaattgag ggatccaaat
     4981 gttatttcaa ctatcaatgt tatgagctta gccgccgtcg gcaagccaaa gttctttgac
     5041 tttgtttcct ccacttctac tcttgacact gaatactact ttaatttgtc agataaactt
     5101 gttagcgaag ggaagccagg cattttagaa tcagacgatt taatgaactc tgcaagcggg
     5161 ctcactggtg gatatggtca gtccaaatgg gctgctgagt acatcattag acgtgcaggt
     5221 gaaaggggcc tacgtgggtg tattgtcaga ccaggttacg taacaggtgc ctctgccaat
     5281 ggttcttcaa acacagatga tttcttattg agatttttga aaggttcagt ccaattaggt
     5341 aagattccag atatcgaaaa ttccgtgaat atggttccag tagatcatgt tgctcgtgtt
     5401 gttgttgcta cgtctttgaa tcctcccaaa gaaaatgaat tggccgttgc tcaagtaacg
     5461 ggtcacccaa gaatattatt caaagactac ttgtatactt tacacgatta tggttacgat
     5521 gtcgaaatcg aaagctattc taaatggaag aaatcattgg aggcgtctgt tattgacagg
     5581 aatgaagaaa atgcgttgta tcctttgcta cacatggtct tagacaactt acctgaaagt
     5641 accaaagctc cggaactaga cgataggaac gccgtggcat ctttaaagaa agacaccgca
     5701 tggacaggtg ttgattggtc taatggaata ggtgttactc cagaagaggt tggtatatat
     5761 attgcatttt taaacaaggt tggattttta cctccaccaa ctcataatga caaacttcca
     5821 ctgccaagta tagaactaac tcaagcgcaa ataagtctag ttgcttcagg tgctggtgct
     5881 cgtggaagct ccgcagcagc ttaaggttga gcattacgta tgatatgtcc atgtacaata
     5941 attaaatatg aattaggaga aagacttagc ttcttttcgg gtgatgtcac ttaaaaactc
     6001 cgagaataat atataataag agaataaaat attagttatt gaataagaac tgtaaatcag
     6061 ctggcgttag tctgctaatg gcagcttcat cttggtttat tgtagcatga atcatatttg
     6121 cctttttttc ctgtaattca atgattcttg cttctatact atcctcaatg caaaaccttg
     6181 tgatcttcac aggtcgatac tgaccaattc tatgaactct atcaccactt tgccattcaa
     6241 cactagggtt ccaccatggg tctaaaatga atacttgcga agctgatcta tggtgcactc
     6301 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
     6361 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     6421 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
     6481 agggcctcgt gatacgccta tttttatagg ttaatgtcat gatagccatc ctcatgaaaa
     6541 ctgtgtaaca taataaccga agtgtcgaaa aggtggcacc ttgtccaatt gaacacgctc
     6601 gatgaaaaaa ataagatata tataaggtta agtaaagcgt ctgttagaaa ggaagttttt
     6661 cctttttctt gctctcttgt cttttcatct actatttcct tcgtgtaata cagggtcgtc
     6721 agatacatag atacaattct attaccccca tccatacaat gccatctcat ttcgatactg
     6781 ttcaactaca cgccggccaa gagaaccctg gtgacaatgc tcacagatcc agagctgtac
     6841 caatttacgc caccacttct tatgttttcg aaaactctaa gcatggttcg caattgtttg
     6901 gtctagaagt tccaggttac gtctattccc gtttccaaaa cccaaccagt aatgttttgg
     6961 aagaaagaat tgctgcttta gaaggtggtg ctgctgcttt ggctgtttcc tccggtcaag
     7021 ccgctcaaac ccttgccatc caaggtttgg cacacactgg tgacaacatc gtttccactt
     7081 cttacttata cggtggtact tataaccagt tcaaaatctc gttcaaaaga tttggtatcg
     7141 aggctagatt tgttgaaggt gacaatccag aagaattcga aaaggtcttt gatgaaagaa
     7201 ccaaggctgt ttatttggaa accattggta atccaaagta caatgttccg gattttgaaa
     7261 aaattgttgc aattgctcac aaacacggta ttccagttgt cgttgacaac acatttggtg
     7321 ccggtggtta cttctgtcag ccaattaaat acggtgctga tattgtaaca cattctgcta
     7381 ccaaatggat tggtggtcat ggtactacta tcggtggtat tattgttgac tctggtaagt
     7441 tcccatggaa ggactaccca gaaaagttcc ctcaattctc tcaacctgcc gaaggatatc
     7501 acggtactat ctacaatgaa gcctacggta acttggcata catcgttcat gttagaactg
     7561 aactattaag agatttgggt ccattgatga acccatttgc ctctttcttg ctactacaag
     7621 gtgttgaaac attatctttg agagctgaaa gacacggtga aaatgcattg aagttagcca
     7681 aatggttaga acaatcccca tacgtatctt gggtttcata ccctggttta gcatctcatt
     7741 ctcatcatga aaatgctaag aagtatctat ctaacggttt cggtggtgtc ttatctttcg
     7801 gtgtaaaaga cttaccaaat gccgacaagg aaactgaccc attcaaactt tctggtgctc
     7861 aagttgttga caatttaaag cttgcctcta acttggccaa tgttggtgat gccaagacct
     7921 tagtcattgc tccatacttc actacccaca aacaattaaa tgacaaagaa aagttggcat
     7981 ctggtgttac caaggactta attcgtgtct ctgttggtat cgaatttatt gatgacatta
     8041 ttgcagactt ccagcaatct tttgaaactg ttttcgctgg ccaaaaacca tgagtgtgcg
     8101 taatgagttg tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     8161 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     8221 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     8281 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     8341 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     8401 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     8461 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     8521 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     8581 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     8641 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     8701 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     8761 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     8821 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     8881 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     8941 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     9001 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     9061 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     9121 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     9181 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     9241 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     9301 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     9361 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     9421 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     9481 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     9541 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     9601 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaa
//
