LOCUS       sequence-239059-        7912 bp    DNA     circular SYN 14-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7912)
  AUTHORS   Beilina A, Van Der Brug M, Ahmad R, Kesavapany S, Miller DW, Petsko 
            GA, Cookson MR
  TITLE     Mutations in PTEN-induced putative kinase 1 associated with 
            recessive parkinsonism have differential effects on protein 
            stability.
  JOURNAL   Proc Natl Acad Sci U S A. 2005 Apr 19. 102(16):5703-8.
  PUBMED    15824318
REFERENCE   2  (bases 1 to 7912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2005 Apr 19. 102(16):5703-8."
COMMENT     Sequence Label: pcDNA-DEST53 PINK1 N-GFP
FEATURES             Location/Qualifiers
     source          1..7912
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(905..907,908..910,911..1621)
                     /codon_start=1
                     /product="Cycle 3 GFP (Crameri et al., 1996)"
                     /label=Cycle 3 GFP
                     /note="mammalian codon-optimized"
                     /translation="MASKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(938..966)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1561..1581
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     protein_bind    1643..1667
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1685..3427
                     /codon_start=1
                     /product="human PTEN-induced putative kinase 1, a 
                     mitochondria serine/threonine protein kinase"
                     /label=PINK1
                     /translation="MAVRQALGRGLQLGRALLLRFTGKPGRAYGLGRPGPAAGCVRGER
                     PGWAAGPGAEPRRVGLGLPNRLRFFRQSVAGLAARLQRQFVVRAWGCAGPCGRAVFLAF
                     GLGLGLIEEKQAESRRAVSACQEIQAIFTQKSKPGPDPLDTRRLQGFRLEEYLIGQSIG
                     KGCSAAVYEATMPTLPQNLEVTKSTGLLPGRGPGTSAPGEGQERAPGAPAFPLAIKMMW
                     NISAGSSSEAILNTMSQELVPASRVALAGEYGAVTYRKSKRGPKQLAPHPNIIRVLRAF
                     TSSVPLLPGALVDYPDVLPSRLHPEGLGHGRTLFLVMKNYPCTLRQYLCVNTPSPRLAA
                     MMLLQLLEGVDHLVQQGIAHRDLKSDNILVELDPDGCPWLVIADFGCCLADESIGLQLP
                     FSSWYVDRGGNGCLMAPEVSTARPGPRAVIDYSKADAWAVGAIAYEIFGLVNPFYGQGK
                     AHLESRSYQEAQLPALPESVPPDVRQLVRALLQREASKRPSARVAANVLHLSLWGEHIL
                     ALKNLKLDKMVGWLLQQSAATLLANRLTEKCCVETKMKMLFLANLECETLCQAALLLCS
                     WRAAL"
     protein_bind    complement(3447..3471)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(3503..3520)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3509..3733
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3779..4207
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3866..3885)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4076..4097
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4216..4236)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4221..4550
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4401..4536
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4463..4482
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4617..5411
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4671..4690)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5281..5300
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5585..5706
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5622..5641)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5676..5695
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5755..5771)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5755..5771)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5768..5790)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5779..5795
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5803..5809,5810..5827,5828..5833))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5848..5869
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5986..6003)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6157..6745)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6237..6256)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6916..7707,7708..7776))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7539..7558
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7777..7881)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctaga
      901 caccatggcc agcaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt
      961 agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgctac
     1021 atacggaaag cttaccctta aatttatttg cactactgga aaactacctg ttccatggcc
     1081 aacacttgtc actactttct cttatggtgt tcaatgcttt tcccgttatc cggatcatat
     1141 gaaacggcat gactttttca agagtgccat gcccgaaggt tatgtacagg aacgcactat
     1201 atctttcaaa gatgacggga actacaagac gcgtgctgaa gtcaagtttg aaggtgatac
     1261 ccttgttaat cgtatcgagt taaaaggtat tgattttaaa gaagatggaa acattctcgg
     1321 acacaaactc gagtacaact ataactcaca caatgtatac atcacggcag acaaacaaaa
     1381 gaatggaatc aaagctaact tcaaaattcg ccacaacatt gaagatggat ccgttcaact
     1441 agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa
     1501 ccattacctg tcgacacaat ctgccctttc gaaagatccc aacgaaaagc gtgaccacat
     1561 ggtccttctt gagtttgtaa ctgctgctgg gattacacat ggcatggatg agctctacaa
     1621 aagcggttcc ggaccggatc aaacaagttt gtacaaaaaa gcaggcttga aggaattcgg
     1681 taccatggcg gtgcgacagg cgctgggccg cggcctgcag ctgggtcgag cgctgctgct
     1741 gcgcttcacg ggcaagcccg gccgggccta cggcttgggg cggccgggcc cggcggcggg
     1801 ctgtgtccgc ggggagcgtc caggctgggc cgcaggaccg ggcgcggagc ctcgcagggt
     1861 cgggctcggg ctccctaacc gtctccgctt cttccgccag tcggtggccg ggctggcggc
     1921 gcggttgcag cggcagttcg tggtgcgggc ctggggctgc gcgggccctt gcggccgggc
     1981 agtctttctg gccttcgggc tagggctggg cctcatcgag gaaaaacagg cggagagccg
     2041 gcgggcggtc tcggcctgtc aggagatcca ggcaattttt acccagaaaa gcaagccggg
     2101 gcctgacccg ttggacacga gacgcttgca gggctttcgg ctggaggagt atctgatagg
     2161 gcagtccatt ggtaagggct gcagtgctgc tgtgtatgaa gccaccatgc ctacattgcc
     2221 ccagaacctg gaggtgacaa agagcaccgg gttgcttcca gggagaggcc caggtaccag
     2281 tgcaccagga gaagggcagg agcgagctcc gggggcccct gccttcccct tggccatcaa
     2341 gatgatgtgg aacatctcgg caggttcctc cagcgaagcc atcttgaaca caatgagcca
     2401 ggagctggtc ccagcgagcc gagtggcctt ggctggggag tatggagcag tcacttacag
     2461 aaaatccaag agaggtccca agcaactagc ccctcacccc aacatcatcc gggttctccg
     2521 cgccttcacc tcttccgtgc cgctgctgcc aggggccctg gtcgactacc ctgatgtgct
     2581 gccctcacgc ctccaccctg aaggcctggg ccatggccgg acgctgttcc tcgttatgaa
     2641 gaactatccc tgtaccctgc gccagtacct ttgtgtgaac acacccagcc cccgcctcgc
     2701 cgccatgatg ctgctgcagc tgctggaagg cgtggaccat ctggttcaac agggcatcgc
     2761 gcacagagac ctgaaatccg acaacatcct tgtggagctg gacccagacg gctgcccctg
     2821 gctggtgatc gcagattttg gctgctgcct ggctgatgag agcatcggcc tgcagttgcc
     2881 cttcagcagc tggtacgtgg atcggggcgg aaacggctgt ctgatggccc cagaggtgtc
     2941 cacggcccgt cctggcccca gggcagtgat tgactacagc aaggctgatg cctgggcagt
     3001 gggagccatc gcctatgaaa tcttcgggct tgtcaatccc ttctacggcc agggcaaggc
     3061 ccaccttgaa agccgcagct accaagaggc tcagctacct gcactgcccg agtcagtgcc
     3121 tccagacgtg agacagttgg tgagggcact gctccagcga gaggccagca agagaccatc
     3181 tgcccgagta gccgcaaatg tgcttcatct aagcctctgg ggtgaacata ttctagccct
     3241 gaagaatctg aagttagaca agatggttgg ctggctcctc caacaatcgg ccgccacttt
     3301 gttggccaac aggctcacag agaagtgttg tgtggaaaca aaaatgaaga tgctctttct
     3361 ggctaacctg gagtgtgaaa cgctctgcca ggcagccctc ctcctctgct catggagggc
     3421 agccctgtag ctcgagtgcg gccgcaaccc agctttcttg tacaaagtgg tgataattaa
     3481 ttaagataaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     3541 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     3601 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     3661 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     3721 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
     3781 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     3841 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     3901 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     3961 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     4021 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     4081 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     4141 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     4201 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     4261 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     4321 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     4381 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     4441 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
     4501 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
     4561 gagcttgtat atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga
     4621 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
     4681 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
     4741 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg
     4801 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
     4861 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
     4921 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
     4981 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
     5041 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
     5101 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg
     5161 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
     5221 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
     5281 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
     5341 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
     5401 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc
     5461 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     5521 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     5581 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     5641 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     5701 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     5761 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     5821 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     5881 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     5941 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     6001 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     6061 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     6121 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     6181 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     6241 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     6301 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     6361 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     6421 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     6481 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     6541 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     6601 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     6661 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     6721 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     6781 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     6841 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     6901 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     6961 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     7021 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     7081 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     7141 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     7201 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     7261 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     7321 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     7381 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     7441 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     7501 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     7561 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     7621 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     7681 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     7741 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     7801 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     7861 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tc
//
