LOCUS       sequence-239190-       15949 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Express NuMA(1-1985)-RFP-Nano from Rosa 26 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15949)
  AUTHORS   Okumura M, Natsume T, Kanemaki MT, Kiyomitsu T
  TITLE     Dynein-Dynactin-NuMA clusters generate cortical spindle-pulling 
            forces as a multi-arm ensemble.
  JOURNAL   Elife. 2018 May 31;7. pii: 36559. doi: 10.7554/eLife.36559.
  PUBMED    29848445
REFERENCE   2  (bases 1 to 15949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-05-31"; volume: "7. pii: 36559. doi"; pages: " 
            10.7554/eLife.36559"
COMMENT     Sequence Label: pTK711
FEATURES             Location/Qualifiers
     source          1..15949
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    1172..1253
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(1370..2116)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     promoter        complement(2135..2645)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     polyA_signal    2665..2713
                     /note="synthetic polyadenylation signal"
     misc_feature    2727..2818
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     2767..2786
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     promoter        2822..3189
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    2830..2848
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2866..2884
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2902..2920
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2938..2956
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2974..2992
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3010..3028
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3046..3064
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     regulatory      9159..9168
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             9165..9896
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
     CDS             9972..10301
                     /codon_start=1
                     /product="SspB from Haemophilus influenzae, with the 
                     mutations Y11K and A15E to disrupt the natural dimerization
                     of the protein"
                     /label=SspB nano
                     /note="dimerizes with iLID at high affinity to create iLID 
                     nano (Guntas et al., 2015)"
                     /translation="SSPKRPKLLREYYDWLVDNSFTPYLVVDATYLGVNVPVEYVKDGQ
                     IVLNLSASATGNLQLTNDFIQFNARFKGVSRELYIPMGAALAIYARENGDGVMFEPEEI
                     YDELNI"
     polyA_signal    10451..10532
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        10554..11064
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     CDS             11101..12120
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             11101..12120
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     primer_bind     complement(12430..12447)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    12436..12660
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(13761..13781)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(13761..13779)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(13800..13816)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13800..13816)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(13813..13835)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    13824..13840
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13848..13854,13855..13872,13873..13878))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13893..13914
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(14202..14790)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(14282..14301)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14961..15752,15753..15821))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     15584..15603
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(15822..15926)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcgg
      661 aactctggtg ggaggggagg gtcagtgaaa ttggctctgg cgcgggcgtc ctcccaccct
      721 ccccttcctt cgggggagtc ggtttacccg ccgcctgctt gtcttcgaca cctgattggc
      781 tgtcgaagct gtgggaccgg gcccttgcta ctggctcgag tctcacatga gcgaaaccac
      841 tgcgcggggc gcgggggtgg cggggaggcg ggcgttggta cggtcctccc cgaggccgag
      901 cgccgcagtg tctggccccg cgcccctgcg caacgtggca ggaagcgcgc gctggaggcg
      961 ggggcgggct gccggccgag acttctggat ggcggcggcc gcggctccgc cccgggttcc
     1021 caccgcctga agggcgagac aagcccgacc tgctacaggc actcgtgggg gtgggggagg
     1081 agcgggggtc ggtccggctg gtttgtgggt gggaggcgct tgttctccaa aaaccggcgc
     1141 gaagctgagt cagtgagcga ggaagctcgg ggcagtgaaa aaaatgcttt atttgtgaaa
     1201 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
     1261 acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca
     1321 agtaaaacct ctacaaatgt ggtatggctg attatgatcc tctggagatt tacccgggga
     1381 gcatgtcaag gtcaaaatcg tcaagagcgt cagcaggcag catatcaagg tcaaagtcgt
     1441 caagggcatc ggctgggagc atgtctaagt caaaatcgtc aagggcgtcg gtcggcccgc
     1501 cgctttcgca ctttagctgt ttctccaggc cacatatgat tagttccagg ccgaaaagga
     1561 aggcaggttc ggctccctgc cggtcgaaca gctcaattgc ttgtttcaga agtgggggca
     1621 tagaatcggt ggtaggtgtc tctctttcct cttttgctac ttgatgctcc tgttcctcca
     1681 atacgcagcc cagtgtaaag tggcccacgg cggacagagc gtacagtgcg ttctccaggg
     1741 agaagccttg ctgacacagg aacgcgagct gattttccag ggtttcgtac tgtttctctg
     1801 ttgggcgggt gccgagatgc actttagccc cgtcgcgatg tgagaggaga gcacagcggt
     1861 atgacttggc gttgttccgc agaaagtctt gccatgactc gccttccagg gggcaggagt
     1921 gggtatgatg cctgtccagc atctcgattg gcagggcatc gagcagggcc cgcttgttct
     1981 tcacgtgcca gtacagggta ggctgctcaa ctcccagctt ttgagcgagt ttccttgtcg
     2041 tcaggccttc gataccgact ccattgagta attccagagc agagtttatg actttgctct
     2101 tgtccagtct agacatggta attcgatgat ccccctgggg agagaggtcg gtgattcggt
     2161 caacgaggga gccgactgcc gacgtgcgct ccggaggctt gcagaatgcg gaacaccgcg
     2221 cgggcaggaa cagggcccac actaccgccc cacaccccgc ctcccgcacc gccccttccc
     2281 ggccgctgct ctcggcgcgc cctgctgagc agccgctatt ggccacagcc catcgcggtc
     2341 ggcgcgctgc cattgctccc tggcgctgtc cgtctgcgag ggtactagtg agacgtgcgg
     2401 cttccgtttg tcacgtccgg cacgccgcga accgcaagga accttcccga cttaggggcg
     2461 gagcaggaag cgtcgccggg gggcccacaa gggtagcggc gaagatccgg gtgacgctgc
     2521 gaacggacgt gaagaatgtg cgagacccag ggtcggcgcc gctgcgtttc ccggaaccac
     2581 gcccagagca gccgcgtccc tgcgcaaacc cagggctgcc ttggaaaagg cgcaacccca
     2641 accccgtgga attatcacct cgagaataaa atatctttat tttcattaca tctgtgtgtt
     2701 ggttttttgt gtgaatcgat agtactaaca tacgctctcc atcaaaacaa aacgaaacaa
     2761 aacaaactag caaaataggc tgtccccagt gcaagtgcag gtgccagaac atttctctct
     2821 cgagtttact ccctatcagt gatagagaac gtatgaagag tttactccct atcagtgata
     2881 gagaacgtat gcagacttta ctccctatca gtgatagaga acgtataagg agtttactcc
     2941 ctatcagtga tagagaacgt atgaccagtt tactccctat cagtgataga gaacgtatct
     3001 acagtttact ccctatcagt gatagagaac gtatatccag tttactccct atcagtgata
     3061 gagaacgtat aagctttgct tatgtaaacc agggcgccta taaaagagtg ctgatttttt
     3121 gagtaaactt caattccaca acacttttgt cttataccaa ctttccgtac cacttcctac
     3181 cctcgtaaag aattcgcggc cgcacgcgtg ccaccatgac actccacgcc acccgggggg
     3241 ctgcactcct ctcttgggtg aacagtctac acgtggctga ccctgtggag gctgtgctgc
     3301 agctccagga ctgcagcatc ttcatcaaga tcattgacag aatccatggc actgaagagg
     3361 gacagcaaat cttgaagcag ccggtgtcag agagactgga ctttgtgtgc agttttctgc
     3421 agaaaaatcg aaaacatccc tcttccccag aatgcctggt atctgcacag aaggtgctag
     3481 agggatcaga gctggaactg gcgaagatga ccatgctgct cttataccac tctaccatga
     3541 gctccaaaag tcccagggac tgggaacagt ttgaatataa aattcaggct gagttggctg
     3601 tcattcttaa atttgtgctg gaccatgagg acgggctaaa ccttaatgag gacctagaga
     3661 acttcctaca gaaagctcct gtgccttcta cctgttctag cacattccct gaagagctct
     3721 ccccacctag ccaccaggcc aagagggaga ttcgcttcct agagctacag aaggttgcct
     3781 cctcttccag tgggaacaac tttctctcag gttctccagc ttctcccatg ggtgatatcc
     3841 tgcagacccc acagttccag atgagacggc tgaagaagca gcttgctgat gagagaagta
     3901 atagggatga gctggagctg gagctagctg agaaccgcaa gctcctcacc gagaaggatg
     3961 cacagatagc catgatgcag cagcgcattg accgcctagc cctgctgaat gagaagcagg
     4021 cggccagccc actggagccc aaggagcttg aggagctgcg tgacaagaat gagagcctta
     4081 ccatgcggct gcatgaaacc ctgaagcagt gccaggacct gaagacagag aagagccaga
     4141 tggatcgcaa aatcaaccag ctttcggagg agaatggaga cctttccttt aagctgcggg
     4201 agtttgccag tcatctgcag cagctacagg atgccctcaa tgagctgacg gaggagcaca
     4261 gcaaggccac tcaggagtgg ctagagaagc aggcccagct ggagaaggag ctcagcgcag
     4321 ccctgcagga caagaaatgc cttgaagaga agaacgaaat ccttcaggga aaactttcac
     4381 agctggaaga acacttgtcc cagctgcagg ataacccacc ccaggagaag ggcgaggtgc
     4441 tgggtgatgt cttgcagctg gaaaccttga agcaagaggc agccactctt gctgcaaaca
     4501 acacacagct ccaagccagg gtagagatgc tggagactga gcgaggccag caggaagcca
     4561 agctgcttgc tgagcggggc cacttcgaag aagaaaagca gcagctgtct agcctgatca
     4621 ctgacctgca gagctccatc tccaacctca gccaggccaa ggaagagctg gagcaggcct
     4681 cccaggctca tggggcccgg ttgactgccc aggtggcctc tctgacctct gagctcacca
     4741 cactcaatgc caccatccag caacaggatc aagaactggc tggcctgaag cagcaggcca
     4801 aagagaagca ggcccagcta gcacagaccc tccaacagca agaacaggcc tcccagggcc
     4861 tccgccacca ggtggagcag ctaagcagta gcctgaagca gaaggagcag cagttgaagg
     4921 aggtagcgga gaagcaggag gcaactaggc aggaccatgc ccagcaactg gccactgctg
     4981 cagaggagcg agaggcctcc ttaagggagc gggatgcggc tctcaagcag ctggaggcac
     5041 tggagaagga gaaggctgcc aagctggaga ttctgcagca gcaacttcag gtggctaatg
     5101 aagcccggga cagtgcccag acctcagtga cacaggccca gcgggagaag gcagagctga
     5161 gccggaaggt ggaggaactc caggcctgtg ttgagacagc ccgccaggaa cagcatgagg
     5221 cccaggccca ggttgcagag ctagagttgc agctgcggtc tgagcagcaa aaagcaactg
     5281 agaaagaaag ggtggcccag gagaaggacc agctccagga gcagctccag gccctcaaag
     5341 agtccttgaa ggtcaccaag ggcagccttg aagaggagaa gcgcagggct gcagatgccc
     5401 tggaagagca gcagcgttgt atctctgagc tgaaggcaga gacccgaagc ctggtggagc
     5461 agcataagcg ggaacgaaag gagctggaag aagagagggc tgggcgcaag gggctggagg
     5521 ctcgattaca gcagcttggg gaggcccatc aggctgagac tgaagtcctg cggcgggagc
     5581 tggcagaggc catggctgcc cagcacacag ctgagagtga gtgtgagcag ctcgtcaaag
     5641 aagtagctgc ctggcgtgag cggtatgagg atagccagca agaggaggca cagtatggcg
     5701 ccatgttcca ggaacagctg atgactttga aggaggaatg tgagaaggcc cgccaggagc
     5761 tgcaggaggc aaaggagaag gtggcaggca tagaatccca cagcgagctc cagataagcc
     5821 ggcagcagaa caaactagct gagctccatg ccaacctggc cagagcactc cagcaggtcc
     5881 aagagaagga agtcagggcc cagaagcttg cagatgacct ctccactctg caggaaaaga
     5941 tggctgccac cagcaaagag gtggcccgct tggagacctt ggtgcgcaag gcaggtgagc
     6001 agcaggaaac agcctcccgg gagttagtca aggagcctgc gagggcagga gacagacagc
     6061 ccgagtggct ggaagagcaa cagggacgcc agttctgcag cacacaggca gcgctgcagg
     6121 ctatggagcg ggaggcagag cagatgggca atgagctgga acggctgcgg gccgcgctga
     6181 tggagagcca ggggcagcag caggaggagc gtgggcagca ggaaagggag gtggcgcggc
     6241 tgacccagga gcggggccgt gcccaggctg accttgccct ggagaaggcg gccagagcag
     6301 agcttgagat gcggctgcag aacgccctca acgagcagcg tgtggagttc gctaccctgc
     6361 aagaggcact ggctcatgcc ctgacggaaa aggaaggcaa ggaccaggag ttggccaagc
     6421 ttcgtggtct ggaggcagcc cagataaaag agctggagga acttcggcaa accgtgaagc
     6481 aactgaagga acagctggct aagaaagaaa aggagcacgc atctggctca ggagcccaat
     6541 ctgaggctgc tggcaggaca gagccaacag gccccaagct ggaagcactg cgggcagagg
     6601 tgagcaagct ggaacagcaa tgccagaagc agcaggagca ggctgacagc ctggaacgca
     6661 gcctcgaggc tgagcgggcc tcccgggctg agcgggacag tgctctggag actctgcagg
     6721 gccagttaga ggagaaggcc caggagctag ggcacagtca gagtgcctta gcctcggccc
     6781 aacgggagtt ggctgccttc cgcaccaagg tacaagacca cagcaaggct gaagatgagt
     6841 ggaaggccca ggtggcccgg ggccggcaag aggctgagag gaaaaatagc ctcatcagca
     6901 gcttggagga ggaggtgtcc atcctgaatc gccaggtcct ggagaaggag ggggagagca
     6961 aggagttgaa gcggctggtg atggccgagt cagagaagag ccagaagctg gaggagaggc
     7021 tgcgcctgct gcaggcagag acagccagca acagtgccag agctgcagaa cgcagctctg
     7081 ctctgcggga ggaggtgcag agcctccggg aggaggctga gaaacagcgg gtggcttcag
     7141 agaacctgcg gcaggagctg acctcacagg ctgagcgtgc ggaggagctg ggccaagaat
     7201 tgaaggcgtg gcaggagaag ttcttccaga aagagcaggc cctctccacc ctgcagctcg
     7261 agcacaccag cacacaggcc ctggtgagtg agctgctgcc agctaagcac ctctgccagc
     7321 agctgcaggc cgagcaggcc gctgccgaga aacgccaccg tgaggagctg gagcagagca
     7381 agcaggccgc tgggggactg cgggcagagc tgctgcgggc ccagcgggag cttggggagc
     7441 tgattcctct gcggcagaag gtggcagagc aggagcgaac agctcagcag ctgcgggcag
     7501 agaaggccag ctatgcagag cagctgagca tgctgaagaa ggcgcatggc ctgctggcag
     7561 aggagaaccg ggggctgggt gagcgggcca accttggccg gcagtttctg gaagtggagt
     7621 tggaccaggc ccgggaaaag tatgtccaag agttggcagc cgtacgtgct gatgctgaga
     7681 cccgtctggc tgaggtgcag cgagaagcac agagcactgc ccgggagctg gaggtgatga
     7741 ctgccaagta tgagggtgcc aaggtcaagg tcctggagga gaggcagcgg ttccaggaag
     7801 agaggcagaa actcactgcc caggtggaag aactgagtaa gaaactggct gactctgacc
     7861 aagccagcaa ggtgcagcag cagaagctga aggctgtcca ggctcaggga ggcgagagcc
     7921 agcaggaggc ccagcgcttc caggcccagc tgaatgaact gcaagcccag ttgagccaga
     7981 aggagcaggc agctgagcac tataagctgc agatggagaa agccaaaaca cattatgatg
     8041 ccaagaagca gcagaaccaa gagctgcagg agcagctgcg gagcctggag cagctgcaga
     8101 aggaaaacaa agagctgcga gctgaagctg aacggctggg ccatgagcta cagcaggctg
     8161 ggctgaagac caaggaggct gaacagacct gccgccacct tactgcccag gtgcgcagcc
     8221 tggaggcaca ggttgcccat gcagaccagc agcttcgaga cctgggcaaa ttccaggtgg
     8281 caactgatgc tttaaagagc cgtgagcccc aggctaagcc ccagctggac ttgagtattg
     8341 acagcctgga tctgagctgc gaggagggga ccccactcag tatcaccagc aagctgcctc
     8401 gtacccagcc agacggcacc agcgtccctg gagaaccagc ctcacctatc tcccagcgcc
     8461 tgccccccaa ggtagaatcc ctggagagtc tctacttcac tcccatccct gctcggagtc
     8521 aggcccccct ggagagcagc ctggactccc tgggagacgt cttcctggac tcgggtcgta
     8581 agacccgctc cgctcgtcgg cgcaccacgc agatcatcaa catcaccatg accaagaagc
     8641 tagatgtgga agagccagac agcgccaact catcgttcta cagcacgcgg tctgctcctg
     8701 cttcccaggc tagcctgcga gccacctcct ctactcagtc tctagctcgc ctgggttctc
     8761 ccgattatgg caactcagcc ctgctcagct tgcctggcta ccgccccacc actcgcagtt
     8821 ctgctcgtcg ttcccaggcc ggggtgtcca gtggggcccc tccaggaagg aacagcttct
     8881 acatgggcac ttgccaggat gagcctgagc agctggatga ctggaaccgc attgcagagc
     8941 tgcagcagcg caatcgagtg tgccccccac atctgaagac ctgctatccc ctggagtcca
     9001 ggccttccct gagcctgggc accatcacag atgaggagat gaaaactgga gacccccaag
     9061 agaccctgcg ccgagccagc atgcagccaa tccagatagc cgagggcact ggcatcacca
     9121 cccggcaggg aggtggatct acgcgtggag gtggatctgc caccatggtg tctaagggcg
     9181 aagagctgat taaggagaac atgcacatga agctgtacat ggagggcacc gtgaacaacc
     9241 accacttcaa gtgcacatcc gagggcgaag gcaagcccta cgagggcacc cagaccatga
     9301 gaatcaaggt ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg gctaccagct
     9361 tcatgtacgg cagcagaacc ttcatcaacc acacccaggg catccccgac ttctttaagc
     9421 agtccttccc tgagggcttc acatgggaga gagtcaccac atacgaagac gggggcgtgc
     9481 tgaccgctac ccaggacacc agcctccagg acggctgcct catctacaac gtcaagatca
     9541 gaggggtgaa cttcccatcc aacggccctg tgatgcagaa gaaaacactc ggctgggagg
     9601 ccaacaccga gatgctgtac cccgctgacg gcggcctgga aggcagaacc gacatggccc
     9661 tgaagctcgt gggcgggggc cacctgatct gcaacttcaa gaccacatac agatccaaga
     9721 aacccgctaa gaacctcaag atgcccggcg tctactatgt ggaccacaga ctggaaagaa
     9781 tcaaggaggc cgacaaagag acctacgtcg agcagcacga ggtggctgtg gccagatact
     9841 gcgacctccc tagcaaactg gggcacaaac ttaatggcat ggacgagctg tacaagtccg
     9901 gactcagatc tcgagctcaa gcttcgaacg aattcggcat tgatctgagc ggcctgaccc
     9961 tgcaggaatt cagctccccg aaacgcccta agctgctgcg tgaatattac gattggctgg
    10021 ttgataacag ctttacccca tatctggtgg tggatgccac atacctgggc gtgaacgtgc
    10081 ccgtggagta tgtgaaagac ggtcagatcg tgctgaatct gtctgcaagt gcgaccggca
    10141 acctgcaact gacaaatgat tttatccagt tcaacgcccg ctttaagggc gtgtctcgtg
    10201 aactgtatat cccgatgggt gccgctctgg ccatttacgc tcgcgagaac ggcgatggtg
    10261 tgatgttcga accagaagaa atctatgacg agctgaatat tggttaaacc ggtggatcca
    10321 gatctctgca gcatatgtct agaggatcat aatcagccat accacatttg tagaggtttt
    10381 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
    10441 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
    10501 aaatttcaca aataaagcat ttttttcact gccgacgtca ggtggcactt ttcggggttg
    10561 gggttgcgcc ttttccaagg cagccctggg tttgcgcagg gacgcggctg ctctgggcgt
    10621 ggttccggga aacgcagcgg cgccgaccct gggtctcgca cattcttcac gtccgttcgc
    10681 agcgtcaccc ggatcttcgc cgctaccctt gtgggccccc cggcgacgct tcctgctccg
    10741 cccctaagtc gggaaggttc cttgcggttc gcggcgtgcc ggacgtgaca aacggaagcc
    10801 gcacgtctca ctagtaccct cgcagacgga cagcgccagg gagcaatggc agcgcgccga
    10861 ccgcgatggg ctgtggccaa tagcggctgc tcagcagggc gcgccgagag cagcggccgg
    10921 gaaggggcgg tgcgggaggc ggggtgtggg gcggtagtgt gggccctgtt cctgcccgcg
    10981 cggtgttccg cattctgcaa gcctccggag cgcacgtcgg cagtcggctc cctcgttgac
    11041 cgaatcaccg acctctctcc ccagggggat ccaccggagc ttaccatgga tagatccgga
    11101 aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc
    11161 tccgacctga tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga
    11221 gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa agatcgttat
    11281 gtttatcggc actttgcatc ggccgcgctc ccgattccgg aagtgcttga cattggggaa
    11341 ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac
    11401 ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg cggaggccat ggatgcgatc
    11461 gctgcggccg atcttagcca gacgagcggg ttcggcccat tcggaccgca aggaatcggt
    11521 caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt gtatcactgg
    11581 caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg
    11641 ctttgggccg aggactgccc cgaagtccgg cacctcgtgc acgcggattt cggctccaac
    11701 aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga ggcgatgttc
    11761 ggggattccc aatacgaggt cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg
    11821 gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc gccgcggctc
    11881 cgggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt tgacggcaat
    11941 ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg
    12001 actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga tggctgtgta
    12061 gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccgagggc aaaggaatag
    12121 agtagatgcc gaccgaacaa gagctgattt cgagaacgcc tcagccagca actcgcgcga
    12181 gcctagcaag gcaaatgcga gagaacggcc ttacgcttgg tggcacagtt ctcgtccaca
    12241 gttcgctaag ctcgctcggc tgggtcgcgg gagggccggt cgcagtgatt caggcccttc
    12301 tggattgtgt tggtccccag ggcacgattg tcatgcccac gcactcgggt gatctgactg
    12361 atcccgcaga ttggagatcg ccgcccgtgc ctgccgattg ggtgcagatc tctagagctc
    12421 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
    12481 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
    12541 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
    12601 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
    12661 cttctgaggc ggaaagaacc agctggggct cgatgaggga gctgcggtgg aggaggtgga
    12721 gagaaggccg cacccttctg ggcaggggga ggggagtgcc gcaatacctt tatgggagtt
    12781 ctctgctgcc tcccgtcttg taaggaccgc cctgggcctg gaagaagccc tccctccttt
    12841 cctcctcgcg tgatctcgtc atcgcctcca tgtcgagtcg cttctcgatt atgggcggga
    12901 ttcttttgcc taggcttaag gggctaactt ggtccctggg cgttgccctg caggggagtg
    12961 agcagctgta agatttgagg ggcgactccg attagtttat cttcccacgg actagagttg
    13021 gtgtcgaggt tattgtaata agggtggggt agggaaatgg agcttagtca ttcacctggg
    13081 gctgatttta tgcaacgaga ctgcggatta tcactaatca tttttggagc atttttctag
    13141 agacagacat aaagcatgat cacctgagtt ttataccatt tgagaccctt gctgcaccac
    13201 caaagtgtag catcaggtta aatcttaata gaaaaatttt agcttttgct tgagaaacca
    13261 gtgcttccct ccctcaccct ctctccccag gctctctacc cctttgcatc cctaccaggc
    13321 atcttagcaa ctctcactca tacttgatcc cattttccat ttgttgtact tgctcctcca
    13381 gtattcagac atagcactag ctttctccct ctcttgatct tgggtagcct ggtgtctcgc
    13441 gaaaccagac agattggttc caccacaaat taaggcttga gctggggctt gactcttacc
    13501 cagcagtgct tttattcctc cctagttcac gttcttaaat gtttatcttg attttcattt
    13561 tatccttttt ccttagctgg gattctgtcc ctgaccgtct tcacagtcca ggtgatcttg
    13621 actactgctt tacagagaat tggatctgag gttaggcaac atctcccttt ttcttcctct
    13681 aaatacctct catttctgtt cttaccagtt agtaactgat ctcagatgcc tgtgtgatag
    13741 ctggtaccca gcttttgttc cctttagtga gggttaattg cgcgcttggc gtaatcatgg
    13801 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
    13861 gggagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
    13921 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
    13981 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
    14041 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
    14101 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
    14161 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
    14221 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
    14281 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
    14341 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
    14401 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
    14461 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
    14521 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
    14581 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
    14641 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
    14701 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
    14761 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
    14821 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
    14881 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
    14941 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
    15001 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
    15061 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
    15121 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
    15181 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
    15241 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
    15301 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
    15361 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
    15421 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
    15481 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
    15541 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
    15601 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
    15661 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
    15721 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
    15781 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
    15841 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
    15901 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccac
//
