LOCUS       sequence-239224-         879 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  pSKAP plasmid containing the unique DNA barcode ""AG"" to be used 
            for allelic exchange on bacterial chromosomes and subsequent 
            molecular identification..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Shaw JA, Bourret TJ
  TITLE     Digital PCR-based Competitive Index for High-throughput Analysis of 
            Fitness in Salmonella.
  JOURNAL   J Vis Exp. 2019 May 13;(147). doi: 10.3791/59630.
  PUBMED    31132072
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Vis 
            Exp."; date: "2019-05-13"; volume: "(147). doi"; pages: " 
            10.3791/59630"
COMMENT     Sequence Label: pSKAP_AG
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..52
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     37..53
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(143..190)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     complement(314..333)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 gmyttmatwa wagggcgatt gggtacgggc ccccctcgag gtcgacggta tcgataagct
       61 tgcatgcaga ttgcagcatt acacgtcttg agagtagtgt cctggaggag catgtgacga
      121 ttgtgtaggc tggagctgct tcgaagttcc tatactttct agagaatagg aacttcggaa
      181 taggaacttc aagatcccct tattagaaga actcgtcaag aaggcgatag aaggcgatgc
      241 gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc
      301 caagctcttc agcaatatca cgggtagcca acgctatgtc ctgatagcgg tccgccacac
      361 ccagccggcc acagtcgatg aatccagaaa agcggccatt ttccaccatg atattcggca
      421 agcaggcatc gccatgggtc acgacgagat cctcgccgtc gggcatgcgc gccttgagcc
      481 tggcgaacag ttcggctggc gcgagcccct gatgctcttc gtccagatca tcctgatcga
      541 caagaccggc ttccatccga gtacgtgctc gctcgatgcg atgtttcgct tggtggtcga
      601 atgggcaggt agccggatca agcgtatgca gccgccgcat tgcatcagcc atgatggata
      661 ctttctcggc aggagcaagg tgagatgaca ggagatcctg ccccggcact tcgcccaata
      721 gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac agctgcgcaa ggaacgcccg
      781 tcgtggccag ccacgatagc cgcgctgcct cgtcctgcag ttcattcagg gcaccggaca
      841 ggtcggtctt gacaaaaaga accgggcgcc ccctgcgct
//
