LOCUS       sequence-239626-       11603 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Mammalian expression plasmid for Env from the Q842.d12 HIV-1 
            isolate; C-terminal truncation; QES mutant with enhanced binding to 
            PG16.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11603)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 11603)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-Q842-QES.i04.c05-754*
FEATURES             Location/Qualifiers
     source          1..11603
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(710..728)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        796..900
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(901..969,970..1761)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1119..1138)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1932..2520
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2421..2440
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2951..3096
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3139..4176
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4174..4193)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4218..4266
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4920..5299
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5300..5503
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5454..5474
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5500..5524
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     polyA_signal    7794..7928
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7818..7837)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7872..7891
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(8071..8093)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8653..10442
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 cctgctcctg ttccaccgtg ggtccctttg cagccaatgc aacttggacg tttttggggt
       61 ctccggacac catctctatg tcttggccct gatcctgagc cgcccggggc tcctggtctt
      121 ccgcctcctc gtcctcgtcc tcttccccgt cctcgtccat ggttatcacc ccctcttctt
      181 tgaggtccac tgccgccgga gccttctggt ccagatgtgt ctcccttctc tcctaggcca
      241 tttccaggtc ctgtacctgg cccctcgtca gacatgattc acactaaaag agatcaatag
      301 acatctttat tagacgacgc tcagtgaata cagggagtgc agactcctgc cccctccaac
      361 agccccccca ccctcatccc cttcatggtc gctgtcagac agatccaggt ctgaaaattc
      421 cccatcctcc gaaccatcct cgtcctcatc accaattact cgcagcccgg aaaactcccg
      481 ctgaacatcc tcaagatttg cgtcctgagc ctcaagccag gcctcaaatt cctcgtcccc
      541 ctttttgctg gacggtaggg atggggattc tcgggacccc tcctcttcct cttcaaggtc
      601 accagacaga gatgctactg gggcaacgga agaaaagctg ggtgcggcct gtgaggatca
      661 gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata
      721 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
      781 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
      841 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
      901 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
      961 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     1021 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     1081 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     1141 cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     1201 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     1261 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     1321 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     1381 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     1441 cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     1501 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     1561 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     1621 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     1681 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     1741 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     1801 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     1861 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     1921 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     1981 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     2041 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     2101 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     2161 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     2221 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     2281 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     2341 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     2401 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     2461 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     2521 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttg aagctgtccc
     2581 tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg
     2641 ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc
     2701 aagacgtagc ccagcgcgtc ggccccgaga tgcgccgcgt gcggctgctg gagatggcgg
     2761 acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt
     2821 gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccct gcttcatccc
     2881 cgtggcccgt tgctcgcgtt tgctggcggt gtccccggaa gaaatatatt tgcatgtctt
     2941 tagttctatg atgacacaaa ccccgcccag cgtcttgtca ttggcgaatt cgaacacgca
     3001 gatgcagtcg gggcggcgcg gtccgaggtc cacttcgcat attaaggtga cgcgtgtggc
     3061 ctcgaacacc gagcgaccct gcagcgaccc gcttaacagc gtcaacagcg tgccgcagat
     3121 cccggggggc aatgagatat gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt
     3181 ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct
     3241 cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc
     3301 gatggtttct acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt
     3361 ccggaagtgc ttgacattgg ggaattcagc gagagcctga cctattgcat ctcccgccgt
     3421 gcacagggtg tcacgttgca agacctgcct gaaaccgaac tgcccgctgt tctgcagccg
     3481 gtcgcggagg ccatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc
     3541 ccattcggac cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt
     3601 gctgatcccc atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc
     3661 gcgcaggctc tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc
     3721 gtgcacgcgg atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc
     3781 attgactgga gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc
     3841 tggaggccgt ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg
     3901 gagcttgcag gatcgccgcg gctccgggcg tatatgctcc gcattggtct tgaccaactc
     3961 tatcagagct tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac
     4021 gcaatcgtcc gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg
     4081 gccgtctgga ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc
     4141 actcgtccgg atcgggagat gggggaggct aactgaaaca cggaaggaga caataccgga
     4201 aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacgggt gttgggtcgt
     4261 ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac
     4321 cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg
     4381 gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata gccactggcc
     4441 ccgtgggtta gggacggggt cccccatggg gaatggttta tggttcgtgg gggttattat
     4501 tttgggcgtt gcgtggggtc aggtccacga ctggactgag cagacagacc catggttttt
     4561 ggatggcctg ggcatggacc gcatgtactg gcgcgacacg aacaccgggc gtctgtggct
     4621 gccaaacacc cccgaccccc aaaaaccacc gcgcggattt ctggcgtgcc aagctagtcg
     4681 accaattctc atgtttgaca gcttatcatc gcagatccgg gcaacgttgt tgccattgct
     4741 gcaggcgcag aactggtagg tatggaagat ctatacattg aatcaatatt ggcaattagc
     4801 catattagtc attggttata tagcataaat caatattggc tattggccat tgcatacgtt
     4861 gtatctatat cataatatgt acatttatat tggctcatgt ccaatatgac cgccatgttg
     4921 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
     4981 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     5041 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     5101 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     5161 agtgtatcat atgccaagtc cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     5221 gcattatgcc cagtacatga ccttacggga ctttcctact tggcagtaca tctacgtatt
     5281 agtcatcgct attaccatgg tgatgcggtt ttggcagtac accaatgggc gtggatagcg
     5341 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
     5401 gcaccaaaat caacgggact ttccaaaatg tcgtaataac cccgccccgt tgacgcaaat
     5461 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca
     5521 gatctctaga agctgggtac cagctgctag cgccaccatg ccaatgggat cactgcagcc
     5581 cttggcaacg ctgtacttgc ttggtatgct tgtggcatct gttcttgctg tcgataattt
     5641 gtgggtcaca gtgtactacg gagtacctgt atggaaagag gcggaaacta ctcttttttg
     5701 cgcgtcagac gcaaaggcat atgagactga aaagcataat gtatgggcta cccatgcgtg
     5761 cgtgcccaca gaccccaacc cccaagaaat ccatctggag aacgttactg aagaatttaa
     5821 tatgtggaag aataatatgg tggaacagat gcatacggac attatcagtc tgtgggatca
     5881 gtctctgaaa ccatgtgtta aactcaccga tctctgcgta accttggact gtaataacgt
     5941 gacgaataat ggaactagtg acatgaggga agagataaag aattgctcct tcaatatgac
     6001 aacagaattg cgagacaagc gacaaaaagt atattctctg ttctacaaat tggacctggt
     6061 gcagataaat gaggatcaag gtaacagctc caataacaag tatcgcctga taacttgcaa
     6121 cacgtctgcg attacccaag catgtcctaa gatgacattt gaacccattc cgattcatta
     6181 ttgcgcacct gcaggctatg ctatcctgaa gtgcaaagat gaagagttta acgggatcgg
     6241 cccgtgcaaa aacgtatcca ctgtccaatg cacccacggt attaagcctg ttgtgtccac
     6301 acagctcctt ctcaatgggt ctctggcaga gaaggaggtc aaaataagat gtgaaaatat
     6361 aacgaacaac gcaaaaccta tcattgtcca actggtgaac ccagttaaaa tcaattgcac
     6421 ccgacctaat aataatacga gaaagagtat ccacatcggc ccagggcagg ccttctacgc
     6481 tacaggcgat ataatcggag acataagaca ggcgcactgc aatgtaaatc gtacggaatg
     6541 gaataatact ctccaccagg tagttgagca gttgcgaaag cattttaata aaacgattaa
     6601 ctttgctaac tcaaccggag gggacttgga aataacaaca cactcattca attgcggggg
     6661 cgaatggttt tattgtaaca cgactaacct gtttaatagc acttggaacc atacggctag
     6721 tatgaactca accgaatcca atgacacaat catcctgccc tgtcgaataa aacaaatcat
     6781 caatatgtgg caaagagtgg gccaagcaat gtatgcaccc ccaataagag gtgtcataag
     6841 gtgtgaatcc aatataactg ggttgattct tactagggat ggaggaaaca cgaacagcac
     6901 ccgagaaact ttccgcccag gcgggggtga tatgagggat aattggcgat cagaacttta
     6961 caagtataag gtagttaaga tagaacccct cggcgttgct cccacaaaag ccaagagaag
     7021 ggttgtagaa cgcgaaaagc gggccgtagg gattggcgct gtattcatcg gatttctcgg
     7081 agcagctgga tctactatgg gtgcagcttc aataacattg acagttcaag cccggcaact
     7141 cctttcaggt attgtacagc agcaaagtaa cctcctgcaa gcgattgagg ctcagcagca
     7201 tctcctgaag cttacggtgt ggggtatcaa acagcttcaa gcgcgggtac tcgcggttga
     7261 gcgatacctt aaggaccaac aattgcttgg aatgtggggc tgcagcggta aacttatatg
     7321 tacaacatct gtgccgtgga acagttcctg gagtaacaaa tcacaaaatg agatttggga
     7381 caatatgacc tggttgcagt gggataaaga aatatcaaac tatacccaga tcatttacga
     7441 tctcttggag gagtctcaaa accagcagga gaaaaacgaa caggacctcc tcgcattgga
     7501 taaatgggcc aatttgtgga actggtttga catatctaac tggctttggt atataaagat
     7561 attcatcatg attgtagggg gtcttattgg tctgaggata gttttcgcag tcttgtcagt
     7621 cattaaccga gtgcggcagg gttattctcc cttgtcattt caaactcaca ctcctaatcc
     7681 gaggggactg gataggcctg agcgaataga ggaagagggc ggtgagcaag acaagaaccg
     7741 gtcaatcagg ctcgtgtctg gtttcctgtg ataactcgag gccggcaagg ccggatccag
     7801 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
     7861 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
     7921 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
     7981 aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat gatccggctg
     8041 cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
     8101 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
     8161 tgttggcggg tgtcggggcg cagccatgag gtcgactcta gaggatcgat ccccgccccg
     8221 gacgaactaa acctgactac gacatctctg ccccttcttc gcggggcagt gcatgtaatc
     8281 ccttcagttg gttggtacaa cttgccaact gggccctgtt ccacatgtga cacggggggg
     8341 gaccaaacac aaaggggttc tctgactgta gttgacatcc ttataaatgg atgtgcacat
     8401 ttgccaacac tgagtggctt tcatcctgga gcagactttg cagtctgtgg actgcaacac
     8461 aacattgcct ttatgtgtaa ctcttggctg aagctcttac accaatgctg ggggacatgt
     8521 acctcccagg ggcccaggaa gactacggga ggctacacca acgtcaatca gaggggcctg
     8581 tgtagctacc gataagcgga ccctcaagag ggcattagca atagtgttta taaggccccc
     8641 ttgttaaccc taaacgggta gcatatgctt cccgggtagt agtatatact atccagacta
     8701 accctaattc aatagcatat gttacccaac gggaagcata tgctatcgaa ttagggttag
     8761 taaaagggtc ctaaggaaca gcgatatctc ccaccccatg agctgtcacg gttttattta
     8821 catggggtca ggattccacg agggtagtga accattttag tcacaagggc agtggctgaa
     8881 gatcaaggag cgggcagtga actctcctga atcttcgcct gcttcttcat tctccttcgt
     8941 ttagctaata gaataactgc tgagttgtga acagtaaggt gtatgtgagg tgctcgaaaa
     9001 caaggtttca ggtgacgccc ccagaataaa atttggacgg ggggttcagt ggtggcattg
     9061 tgctatgaca ccaatataac cctcacaaac cccttgggca ataaatacta gtgtaggaat
     9121 gaaacattct gaatatcttt aacaatagaa atccatgggg tggggacaag ccgtaaagac
     9181 tggatgtcca tctcacacga atttatggct atgggcaaca cataatccta gtgcaatatg
     9241 atactggggt tattaagatg tgtcccaggc agggaccaag acaggtgaac catgttgtta
     9301 cactctattt gtaacaaggg gaaagagagt ggacgccgac agcagcggac tccactggtt
     9361 gtctctaaca cccccgaaaa ttaaacgggg ctccacgcca atggggccca taaacaaaga
     9421 caagtggcca ctcttttttt tgaaattgtg gagtgggggc acgcgtcagc ccccacacgc
     9481 cgccctgcgg ttttggactg taaaataagg gtgtaataac ttggctgatt gtaaccccgc
     9541 taaccactgc ggtcaaacca cttgcccaca aaaccactaa tggcaccccg gggaatacct
     9601 gcataagtag gtgggcgggc caagataggg gcgcgattgc tgcgatctgg aggacaaatt
     9661 acacacactt gcgcctgagc gccaagcaca gggttgttgg tcctcatatt cacgaggtcg
     9721 ctgagagcac ggtgggctaa tgttgccatg ggtagcatat actacccaaa tatctggata
     9781 gcatatgcta tcctaatcta tatctgggta gcataggcta tcctaatcta tatctgggta
     9841 gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaattta tatctgggta
     9901 gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     9961 gtatatgcta tcctaatctg tatccgggta gcatatgcta tcctaataga gattagggta
    10021 gtatatgcta tcctaattta tatctgggta gcatatacta cccaaatatc tggatagcat
    10081 atgctatcct aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagcat
    10141 aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat
    10201 atgctatcct aatttatatc tgggtagcat aggctatcct aatctatatc tgggtagcat
    10261 atgctatcct aatctatatc tgggtagtat atgctatcct aatctgtatc cgggtagcat
    10321 atgctatcct catgcatata cagtcagcat atgataccca gtagtagagt gggagtgcta
    10381 tcctttgcat atgccgccac ctcccaaggg ggcgtgaatt ttcgctgctt gtccttttcc
    10441 tgctggttgc tcccattctt aggtgaattt aaggaggcca ggctaaagcc gtcgcatgtc
    10501 tgattgctca ccaggtaaat gtcgctaatg ttttccaacg cgagaaggtg ttgagcgcgg
    10561 agctgagtga cgtgacaaca tgggtatgcc caattgcccc atgttgggag gacgaaaatg
    10621 gtgacaagac agatggccag aaatacacca acagcacgca tgatgtctac tggggattta
    10681 ttctttagtg cgggggaata cacggctttt aatacgattg agggcgtctc ctaacaagtt
    10741 acatcactcc tgcccttcct caccctcatc tccatcacct ccttcatctc cgtcatctcc
    10801 gtcatcaccc tccgcggcag ccccttccac cataggtgga aaccagggag gcaaatctac
    10861 tccatcgtca aagctgcaca cagtcaccct gatattgcag gtaggagcgg gctttgtcat
    10921 aacaaggtcc ttaatcgcat ccttcaaaac ctcagcaaat atatgagttt gtaaaaagac
    10981 catgaaataa cagacaatgg actcccttag cgggccaggt tgtgggccgg gtccaggggc
    11041 cattccaaag gggagacgac tcaatggtgt aagacgacat tgtggaatag caagggcagt
    11101 tcctcgcctt aggttgtaaa gggaggtctt actacctcca tatacgaaca caccggcgac
    11161 ccaagttcct tcgtcggtag tcctttctac gtgactccta gccaggagag ctcttaaacc
    11221 ttctgcaatg ttctcaaatt tcgggttgga acctccttga ccacgatgct ttccaaacca
    11281 ccctcctttt ttgcgcctgc ctccatcacc ctgaccccgg ggtccagtgc ttgggccttc
    11341 tcctgggtca tctgcggggc cctgctctat cgctcccggg ggcacgtcag gctcaccatc
    11401 tgggccacct tcttggtggt attcaaaata atcggcttcc cctacagggt ggaaaaatgg
    11461 ccttctacct ggagggggcc tgcgcggtgg agacccggat gatgatgact gactactggg
    11521 actcctgggc ctcttttctc cacgtccacg acctctcccc ctggctcttt cacgacttcc
    11581 ccccctggct ctttcacgtc ctc
//
