LOCUS       sequence-239629-       11976 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Mammalian expression plasmid for Env from the B41 HIV-1 isolate; QES
            mutant with enhanced binding to PG16.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11976)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 11976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-B41gp160-V200E
FEATURES             Location/Qualifiers
     source          1..11976
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(711..729)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        797..901
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(902..970,971..1762)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1120..1139)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1933..2521
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2422..2441
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2952..3097
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3140..4177
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4175..4194)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4219..4267
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4921..5300
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5301..5504
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5455..5475
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5501..5525
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             7138..7149
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     polyA_signal    8164..8298
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8188..8207)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8242..8261
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(8441..8463)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9023..10812
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 tcctgctcct gttccaccgt gggtcccttt gcagccaatg caacttggac gtttttgggg
       61 tctccggaca ccatctctat gtcttggccc tgatcctgag ccgcccgggg ctcctggtct
      121 tccgcctcct cgtcctcgtc ctcttccccg tcctcgtcca tggttatcac cccctcttct
      181 ttgaggtcca ctgccgccgg agccttctgg tccagatgtg tctcccttct ctcctaggcc
      241 atttccaggt cctgtacctg gcccctcgtc agacatgatt cacactaaaa gagatcaata
      301 gacatcttta ttagacgacg ctcagtgaat acagggagtg cagactcctg ccccctccaa
      361 cagccccccc accctcatcc ccttcatggt cgctgtcaga cagatccagg tctgaaaatt
      421 ccccatcctc cgaaccatcc tcgtcctcat caccaattac tcgcagcccg gaaaactccc
      481 gctgaacatc ctcaagattt gcgtcctgag cctcaagcca ggcctcaaat tcctcgtccc
      541 cctttttgct ggacggtagg gatggggatt ctcgggaccc ctcctcttcc tcttcaaggt
      601 caccagacag agatgctact ggggcaacgg aagaaaagct gggtgcggcc tgtgaggatc
      661 agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat
      721 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
      781 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
      841 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
      901 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
      961 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     1021 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     1081 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     1141 ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     1201 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     1261 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     1321 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     1381 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     1441 gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     1501 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     1561 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     1621 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     1681 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     1741 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     1801 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     1861 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     1921 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     1981 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     2041 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     2101 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     2161 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     2221 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     2281 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     2341 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     2401 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     2461 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     2521 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt gaagctgtcc
     2581 ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc
     2641 gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag
     2701 caagacgtag cccagcgcgt cggccccgag atgcgccgcg tgcggctgct ggagatggcg
     2761 gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat
     2821 tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccc tgcttcatcc
     2881 ccgtggcccg ttgctcgcgt ttgctggcgg tgtccccgga agaaatatat ttgcatgtct
     2941 ttagttctat gatgacacaa accccgccca gcgtcttgtc attggcgaat tcgaacacgc
     3001 agatgcagtc ggggcggcgc ggtccgaggt ccacttcgca tattaaggtg acgcgtgtgg
     3061 cctcgaacac cgagcgaccc tgcagcgacc cgcttaacag cgtcaacagc gtgccgcaga
     3121 tcccgggggg caatgagata tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt
     3181 tctgatcgaa aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc
     3241 tcgtgctttc agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc
     3301 cgatggtttc tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat
     3361 tccggaagtg cttgacattg gggaattcag cgagagcctg acctattgca tctcccgccg
     3421 tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctgcagcc
     3481 ggtcgcggag gccatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg
     3541 cccattcgga ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat
     3601 tgctgatccc catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt
     3661 cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct
     3721 cgtgcacgcg gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt
     3781 cattgactgg agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt
     3841 ctggaggccg tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc
     3901 ggagcttgca ggatcgccgc ggctccgggc gtatatgctc cgcattggtc ttgaccaact
     3961 ctatcagagc ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga
     4021 cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc
     4081 ggccgtctgg accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag
     4141 cactcgtccg gatcgggaga tgggggaggc taactgaaac acggaaggag acaataccgg
     4201 aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggg tgttgggtcg
     4261 tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga
     4321 ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg
     4381 ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat agccactggc
     4441 cccgtgggtt agggacgggg tcccccatgg ggaatggttt atggttcgtg ggggttatta
     4501 ttttgggcgt tgcgtggggt caggtccacg actggactga gcagacagac ccatggtttt
     4561 tggatggcct gggcatggac cgcatgtact ggcgcgacac gaacaccggg cgtctgtggc
     4621 tgccaaacac ccccgacccc caaaaaccac cgcgcggatt tctggcgtgc caagctagtc
     4681 gaccaattct catgtttgac agcttatcat cgcagatccg ggcaacgttg ttgccattgc
     4741 tgcaggcgca gaactggtag gtatggaaga tctatacatt gaatcaatat tggcaattag
     4801 ccatattagt cattggttat atagcataaa tcaatattgg ctattggcca ttgcatacgt
     4861 tgtatctata tcataatatg tacatttata ttggctcatg tccaatatga ccgccatgtt
     4921 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
     4981 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
     5041 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
     5101 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     5161 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
     5221 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
     5281 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
     5341 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
     5401 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
     5461 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
     5521 agatctctag aagctgggta ccagctgcta gcgccaccat gccaatgggt agtcttcagc
     5581 ctctcgccac cttgtatctg ctcggtatgc tggttgccag cgttctggct aacttgtggg
     5641 tgaccgtata ctacggggtc ccggtatgga aggaggcaaa cactacactc ttctgtgctt
     5701 ccgacgcaaa ggggtatgat acggagaagc ataatgtctg ggcgacacat gcatgtgtgc
     5761 ctacagaccc aaacccgcag gaagtcctcc ttggtaataa cgtgaccgag aactttaata
     5821 tgtggaaaaa taacatggta gagcagatgc atgaagacat tataagcttg tgggatcaat
     5881 ctcttaagcc gtgtgtgaag ctgacaccgc tttgcgtaac actcaattgt acggatttga
     5941 cgaaaaatag cactaccaat aactccaccg ctactaacaa taccacgaac aatagtaccg
     6001 aaaaaatgga gatggacaaa ggcgagatga agaattgctc ctttaatatc acaacaaata
     6061 ttcgagataa gatgcagaaa gaatacgctt tgctgtacaa gcttgacatc gtgccgatcg
     6121 acaatggcaa gaataacagc aacaccaatt atcgcctgat ttcatgtaat acctcagaga
     6181 ttacgcaatc atgtcccaaa gtgtcatttc agcccattcc tattcattat tgtgcacccg
     6241 ccggttttgc aatacttaag tgtaataata agacctttaa tggaagcggg ccttgtacta
     6301 atgtgtcttc cgtgcaatgc actcacggta tccgacctgt tgtttcaacc cagcttttgc
     6361 tgaacggaag tctcgctgaa gaagaaatcg tgattaggtc cgctaacttc tctgacaaca
     6421 cgaagactat aattgtccaa cttaaggaag cggtcgaaat aaactgtacg cgcccgaata
     6481 ataacacgcg aaaatcaatt aacatcgggc ctgggcgagc tttttatgct actggcaaca
     6541 taatagggga catccgccaa gctcattgta acatatcacg gacaaagtgg aataatacgt
     6601 tgacagaaat tgctaagaag ctgaacaagc agtacgagaa tagaacaata gcatttaatc
     6661 aatcctccgg aggcgatctt gagatcgtca tgcattcatt caactgcggt ggagaatttt
     6721 tctattgcaa cacttcccag ctgttcagag atacgtggaa caacacttgg aacggcatat
     6781 ggaacaacga tacggaggtc aacgagacta tcacacttcc gtgtagaatc aaacaaataa
     6841 tcaatatgtg gcaagaagtc gggaaggcga tgtatgcccc gccaatccgc ggccaaatca
     6901 attgctcatc aaatattacg ggactcctgc tcacccgcga cgggggagaa agcaacaaga
     6961 ccatagaggt cttccggccc ggtggcggag acatgaggga caactggagg tccgagctgt
     7021 ataagtacaa agttgttaag atagagcccc tcggggttgc tcccaccaaa gctaaaagaa
     7081 gagtggtgca acgagagaaa agagccgtcg gcttgggtgc cctcttcgtc ggatttttgg
     7141 gagctgcagg tagcacaatg ggtgctgcct ctataacgct gaccgtacaa gctcgacagc
     7201 ttcttagtgg tatcgttcaa caacaaaaca atctcttgcg agcaatagaa gcccaacagc
     7261 atttgcttca actgacagta tggggcgtta agcagttgca agcgcgggtc ttggcggtcg
     7321 aacgatacct gaaagaccag caactcctgg gcttgtgggg atgcagcggc aaacttatat
     7381 gtaccacagc tgtaccttgg aacaccagct ggagtaacaa gagttacgat tatatctggg
     7441 acaacatgac gtggatgcaa tgggagcgcg agattgataa ctatacagga gttatctaca
     7501 agctgattga ggaatcccag aaccaacagg agaaaaatga acaagaactt ttggccctgg
     7561 ataaatgggc aaatctgtgg aactggtttg acatcacaag atggctttgg tacattaaga
     7621 tttttattat gatcgtaggg ggtttggtag ggcttcggat cgtttttgct gtgctctcca
     7681 tcgtgaaccg agtcaggcag gggtactcac ctttgagcct gcaaacgcac tttcctgcgc
     7741 caagaggacc ggatcgacca gagggaacgg aggaggaagg tggtgaccgg gatcgggatc
     7801 gcagcactcc cctcgtggat gggttccttg caataatttg ggtagacctt aggagtttgt
     7861 ttcttttctc ctaccaccgc ctgagagacc tcctcctgat agtgacacgg attgtcgaac
     7921 tgcttggtcg cagaggttgg gaggccctta agtactggtg gaatcttctt cagtattggt
     7981 cccaagagct taggaactct gcggtctcct tgttcaatgc cacagcgatc gcagtggctg
     8041 agggaaccga ccgagtcatt gaggcggtcc aaagaattgg ccgggcaatt cttcacatac
     8101 ctcgaagaat aaggcagggc tttgagagag cactgcagta gtaactcgag gccggcaagg
     8161 ccggatccag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
     8221 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
     8281 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
     8341 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat
     8401 gatccggctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     8461 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     8521 cgtcagcggg tgttggcggg tgtcggggcg cagccatgag gtcgactcta gaggatcgat
     8581 ccccgccccg gacgaactaa acctgactac gacatctctg ccccttcttc gcggggcagt
     8641 gcatgtaatc ccttcagttg gttggtacaa cttgccaact gggccctgtt ccacatgtga
     8701 cacggggggg gaccaaacac aaaggggttc tctgactgta gttgacatcc ttataaatgg
     8761 atgtgcacat ttgccaacac tgagtggctt tcatcctgga gcagactttg cagtctgtgg
     8821 actgcaacac aacattgcct ttatgtgtaa ctcttggctg aagctcttac accaatgctg
     8881 ggggacatgt acctcccagg ggcccaggaa gactacggga ggctacacca acgtcaatca
     8941 gaggggcctg tgtagctacc gataagcgga ccctcaagag ggcattagca atagtgttta
     9001 taaggccccc ttgttaaccc taaacgggta gcatatgctt cccgggtagt agtatatact
     9061 atccagacta accctaattc aatagcatat gttacccaac gggaagcata tgctatcgaa
     9121 ttagggttag taaaagggtc ctaaggaaca gcgatatctc ccaccccatg agctgtcacg
     9181 gttttattta catggggtca ggattccacg agggtagtga accattttag tcacaagggc
     9241 agtggctgaa gatcaaggag cgggcagtga actctcctga atcttcgcct gcttcttcat
     9301 tctccttcgt ttagctaata gaataactgc tgagttgtga acagtaaggt gtatgtgagg
     9361 tgctcgaaaa caaggtttca ggtgacgccc ccagaataaa atttggacgg ggggttcagt
     9421 ggtggcattg tgctatgaca ccaatataac cctcacaaac cccttgggca ataaatacta
     9481 gtgtaggaat gaaacattct gaatatcttt aacaatagaa atccatgggg tggggacaag
     9541 ccgtaaagac tggatgtcca tctcacacga atttatggct atgggcaaca cataatccta
     9601 gtgcaatatg atactggggt tattaagatg tgtcccaggc agggaccaag acaggtgaac
     9661 catgttgtta cactctattt gtaacaaggg gaaagagagt ggacgccgac agcagcggac
     9721 tccactggtt gtctctaaca cccccgaaaa ttaaacgggg ctccacgcca atggggccca
     9781 taaacaaaga caagtggcca ctcttttttt tgaaattgtg gagtgggggc acgcgtcagc
     9841 ccccacacgc cgccctgcgg ttttggactg taaaataagg gtgtaataac ttggctgatt
     9901 gtaaccccgc taaccactgc ggtcaaacca cttgcccaca aaaccactaa tggcaccccg
     9961 gggaatacct gcataagtag gtgggcgggc caagataggg gcgcgattgc tgcgatctgg
    10021 aggacaaatt acacacactt gcgcctgagc gccaagcaca gggttgttgg tcctcatatt
    10081 cacgaggtcg ctgagagcac ggtgggctaa tgttgccatg ggtagcatat actacccaaa
    10141 tatctggata gcatatgcta tcctaatcta tatctgggta gcataggcta tcctaatcta
    10201 tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaattta
    10261 tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta
    10321 tatctgggta gtatatgcta tcctaatctg tatccgggta gcatatgcta tcctaataga
    10381 gattagggta gtatatgcta tcctaattta tatctgggta gcatatacta cccaaatatc
    10441 tggatagcat atgctatcct aatctatatc tgggtagcat atgctatcct aatctatatc
    10501 tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc
    10561 tgggtagtat atgctatcct aatttatatc tgggtagcat aggctatcct aatctatatc
    10621 tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct aatctgtatc
    10681 cgggtagcat atgctatcct catgcatata cagtcagcat atgataccca gtagtagagt
    10741 gggagtgcta tcctttgcat atgccgccac ctcccaaggg ggcgtgaatt ttcgctgctt
    10801 gtccttttcc tgctggttgc tcccattctt aggtgaattt aaggaggcca ggctaaagcc
    10861 gtcgcatgtc tgattgctca ccaggtaaat gtcgctaatg ttttccaacg cgagaaggtg
    10921 ttgagcgcgg agctgagtga cgtgacaaca tgggtatgcc caattgcccc atgttgggag
    10981 gacgaaaatg gtgacaagac agatggccag aaatacacca acagcacgca tgatgtctac
    11041 tggggattta ttctttagtg cgggggaata cacggctttt aatacgattg agggcgtctc
    11101 ctaacaagtt acatcactcc tgcccttcct caccctcatc tccatcacct ccttcatctc
    11161 cgtcatctcc gtcatcaccc tccgcggcag ccccttccac cataggtgga aaccagggag
    11221 gcaaatctac tccatcgtca aagctgcaca cagtcaccct gatattgcag gtaggagcgg
    11281 gctttgtcat aacaaggtcc ttaatcgcat ccttcaaaac ctcagcaaat atatgagttt
    11341 gtaaaaagac catgaaataa cagacaatgg actcccttag cgggccaggt tgtgggccgg
    11401 gtccaggggc cattccaaag gggagacgac tcaatggtgt aagacgacat tgtggaatag
    11461 caagggcagt tcctcgcctt aggttgtaaa gggaggtctt actacctcca tatacgaaca
    11521 caccggcgac ccaagttcct tcgtcggtag tcctttctac gtgactccta gccaggagag
    11581 ctcttaaacc ttctgcaatg ttctcaaatt tcgggttgga acctccttga ccacgatgct
    11641 ttccaaacca ccctcctttt ttgcgcctgc ctccatcacc ctgaccccgg ggtccagtgc
    11701 ttgggccttc tcctgggtca tctgcggggc cctgctctat cgctcccggg ggcacgtcag
    11761 gctcaccatc tgggccacct tcttggtggt attcaaaata atcggcttcc cctacagggt
    11821 ggaaaaatgg ccttctacct ggagggggcc tgcgcggtgg agacccggat gatgatgact
    11881 gactactggg actcctgggc ctcttttctc cacgtccacg acctctcccc ctggctcttt
    11941 cacgacttcc ccccctggct ctttcacgtc ctctac
//
