LOCUS       sequence-23968-e         805 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Henzler-Wildman KA, Thai V, Lei M, Ott M, Wolf-Watz M, Fenn T, 
            Pozharski E, Wilson MA, Petsko GA, Karplus M, Hubner CG, Kern D
  TITLE     Intrinsic motions along an enzymatic reaction trajectory.
  JOURNAL   Nature. 2007 Dec 6. 450(7171):838-44.
  PUBMED    18026086
REFERENCE   2  (bases 1 to 805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2007 Dec 6. 450(7171):838-44."
COMMENT     Sequence Label: peT3a-AqAdk_MVGDH
FEATURES             Location/Qualifiers
     source          1..805
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..52)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             689..711
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ctnctttcgg gctttgttag cagccggatc ctcagtggtg gtggtggtgg tgattgccat
       61 ctcctataac ctctaaaact tgcctgtaaa cctcctcaac aggtttactt gcgtctatta
      121 ttcttaatat acctttcttc ttgtaatatt caatgagggg agcggtttgc tctctgtaaa
      181 cctcgagtct ctttttaatg acttcgggtt tgtcgtcttc cctttgaata accttacaac
      241 cgggaggcgg tgggttgtac ttcacgtggt aaacctctcc cgtttcggga tttatccttc
      301 ttcctgaaag cctttctatt accacctcgt cgggaacttc aaagaggaga acatggtcaa
      361 ctttaagtcc tttcttttca agcatctcgt caagggcctc tgcctgtttt acggttctgg
      421 gaaagccgtc aaatatgacg tttccgtgct tcggaaatac ttcctctatt aaagctatta
      481 ttaagtcatc gggaacgagt tctccccttt ccatgcattc tttggctttt ttaccgaggg
      541 gtgttccctt ctgaacggct tcccttaaga tatctcctgt ggatatgtgg acaaagccct
      601 tctcttggnn gnnncctnnn nnnctgagtt ccttttcctg gcaccgggag gtcctaggaa
      661 aacccagtat catgcctacc atatggcctc tccttcttaa agttaaacaa aattatttct
      721 agagggaacc gtgtgnctcc taaggngcga naaantccgg naacagactg atctcancgg
      781 agacggggcg accgggcaag gcgtg
//
