LOCUS       sequence-239699-       11887 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Mammalian expression plasmid for Env from the AD8 HIV-1 isolate.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11887)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 11887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-AD8gp160
FEATURES             Location/Qualifiers
     source          1..11887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(751..769)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        837..941
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(942..1010,1011..1802)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1160..1179)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1973..2561
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2462..2481
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2992..3137
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3180..4217
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4215..4234)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4259..4307
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4961..5340
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5341..5544
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5495..5515
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5541..5565
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             7540..7584
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     polyA_signal    8171..8305
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8195..8214)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8249..8268
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(8448..8470)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9030..10819
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 ctgctcctgc ccctcctgct cctgcccctc ccgctcctgc tcctgctcct gttccaccgt
       61 gggtcccttt gcagccaatg caacttggac gtttttgggg tctccggaca ccatctctat
      121 gtcttggccc tgatcctgag ccgcccgggg ctcctggtct tccgcctcct cgtcctcgtc
      181 ctcttccccg tcctcgtcca tggttatcac cccctcttct ttgaggtcca ctgccgccgg
      241 agccttctgg tccagatgtg tctcccttct ctcctaggcc atttccaggt cctgtacctg
      301 gcccctcgtc agacatgatt cacactaaaa gagatcaata gacatcttta ttagacgacg
      361 ctcagtgaat acagggagtg cagactcctg ccccctccaa cagccccccc accctcatcc
      421 ccttcatggt cgctgtcaga cagatccagg tctgaaaatt ccccatcctc cgaaccatcc
      481 tcgtcctcat caccaattac tcgcagcccg gaaaactccc gctgaacatc ctcaagattt
      541 gcgtcctgag cctcaagcca ggcctcaaat tcctcgtccc cctttttgct ggacggtagg
      601 gatggggatt ctcgggaccc ctcctcttcc tcttcaaggt caccagacag agatgctact
      661 ggggcaacgg aagaaaagct gggtgcggcc tgtgaggatc agcttatcga tgataagctg
      721 tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta
      781 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
      841 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
      901 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
      961 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     1021 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     1081 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     1141 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag
     1201 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     1261 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     1321 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     1381 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     1441 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa
     1501 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     1561 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     1621 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     1681 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     1741 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     1801 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     1861 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     1921 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     1981 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     2041 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     2101 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     2161 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     2221 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     2281 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     2341 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     2401 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     2461 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     2521 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     2581 ttttacggtt cctggccttt tgctggcctt gaagctgtcc ctgatggtcg tcatctacct
     2641 gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca
     2701 taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt
     2761 cggccccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg
     2821 ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg
     2881 agtggtgaat ccgttagcga ggtgccgccc tgcttcatcc ccgtggcccg ttgctcgcgt
     2941 ttgctggcgg tgtccccgga agaaatatat ttgcatgtct ttagttctat gatgacacaa
     3001 accccgccca gcgtcttgtc attggcgaat tcgaacacgc agatgcagtc ggggcggcgc
     3061 ggtccgaggt ccacttcgca tattaaggtg acgcgtgtgg cctcgaacac cgagcgaccc
     3121 tgcagcgacc cgcttaacag cgtcaacagc gtgccgcaga tcccgggggg caatgagata
     3181 tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca
     3241 gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg
     3301 taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc
     3361 gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg cttgacattg
     3421 gggaattcag cgagagcctg acctattgca tctcccgccg tgcacagggt gtcacgttgc
     3481 aagacctgcc tgaaaccgaa ctgcccgctg ttctgcagcc ggtcgcggag gccatggatg
     3541 cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga ccgcaaggaa
     3601 tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc catgtgtatc
     3661 actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc
     3721 tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg gatttcggct
     3781 ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg agcgaggcga
     3841 tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg tggttggctt
     3901 gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc
     3961 ggctccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc ttggttgacg
     4021 gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag
     4081 ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg accgatggct
     4141 gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg gatcgggaga
     4201 tgggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg
     4261 gcaataaaaa gacagaataa aacgcacggg tgttgggtcg tttgttcata aacgcggggt
     4321 tcggtcccag ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc
     4381 ccgcgtttct tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc
     4441 agccaacgtc ggggcggcag gccctgccat agccactggc cccgtgggtt agggacgggg
     4501 tcccccatgg ggaatggttt atggttcgtg ggggttatta ttttgggcgt tgcgtggggt
     4561 caggtccacg actggactga gcagacagac ccatggtttt tggatggcct gggcatggac
     4621 cgcatgtact ggcgcgacac gaacaccggg cgtctgtggc tgccaaacac ccccgacccc
     4681 caaaaaccac cgcgcggatt tctggcgtgc caagctagtc gaccaattct catgtttgac
     4741 agcttatcat cgcagatccg ggcaacgttg ttgccattgc tgcaggcgca gaactggtag
     4801 gtatggaaga tctatacatt gaatcaatat tggcaattag ccatattagt cattggttat
     4861 atagcataaa tcaatattgg ctattggcca ttgcatacgt tgtatctata tcataatatg
     4921 tacatttata ttggctcatg tccaatatga ccgccatgtt gacattgatt attgactagt
     4981 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
     5041 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
     5101 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
     5161 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
     5221 ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     5281 accttacggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     5341 gtgatgcggt tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt
     5401 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
     5461 tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg
     5521 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatctctag aagctgggta
     5581 ccagctgcta gcgccaccat gccaatgggt tcactgcaac cacttgcgac gctgtacctc
     5641 ttgggcatgc tggtggcatc cgttctggca gttgagaatc tctgggtcac tgtttactac
     5701 ggcgtgcccg tatggaagga agccacgacg acattgttct gtgcgagcga tgcgaaggca
     5761 tacgacactg aggtccacaa tgtctgggca acacacgcgt gcgtccctac cgaccccaat
     5821 ccccaggaag tggtccttga aaatgtcact gagaatttta atatgtggaa gaataatatg
     5881 gttgagcaga tgcatgagga catcatttct ctgtgggatc agtccctcaa accttgtgtc
     5941 aagttgacgc ctctgtgtgt aaccctcaac tgcaccgatt tgaggaatgt aaccaatatc
     6001 aacaattcta gtgaaggtat gaggggagag atcaaaaact gctccttcaa cataaccacc
     6061 tccattcgag acaaggtaaa aaaggattac gctctctttt atagattgga cgtggtaccc
     6121 attgacaatg ataacaccag ttataggctg ataaactgta atacctcaac gattacacaa
     6181 gcttgtccta aagtatcatt cgaacccatt ccgatccatt actgtacgcc ggccggtttc
     6241 gccatcctta aatgtaagga taagaaattc aatggtactg gcccgtgcaa aaatgtatct
     6301 acggtccagt gtacacacgg cataagaccc gtagtatcta cgcaactcct gcttaacggg
     6361 agcctggccg aagaggaagt tgtaatcaga agtagtaact tcactgataa tgccaagaac
     6421 atcattgtgc agttgaaaga gtcagtcgag atcaactgta ccaggccgaa caataataca
     6481 cgaaagtcaa tacacatagg gcccggaagg gcattctaca ccactgggga tataatcgga
     6541 gatatacgcc aagcgcattg caatatcagt aggaccaagt ggaacaatac gctgaaccaa
     6601 atcgctacaa agcttaagga acaatttggg aataacaaga caatagtatt taatcaatca
     6661 tctgggggcg acccggagat agtcatgcat tcatttaatt gcggcggaga atttttttat
     6721 tgtaattcca cacagctttt taatagcaca tggaatttca acggaacatg gaatctgaca
     6781 cagtccaatg gtacggaggg gaatgacaca attacccttc cctgtcgcat taaacagata
     6841 attaatatgt ggcaagaagt cggaaaggcg atgtatgctc ctcctattcg agggcagata
     6901 aggtgctcat caaatatcac cggcctcata ctgactcggg acggcggaaa taatcacaac
     6961 aatgacactg agacatttcg cccaggtgga ggtgatatgc gggataactg gcgctccgaa
     7021 ttgtacaagt acaaagttgt caagattgag ccactcggag tggccccgac aaaggctaag
     7081 aggcgggtcg tacaaaggga gaaacgcgcg gtaggaacca ttggagccat gttcttgggt
     7141 ttcttgggcg cagcgggatc caccatgggg gcggcctcta tcacactgac cgtgcaggca
     7201 agactcctct tgagcggcat cgtgcaacag caaaacaatc tcttgcgcgc tattgaggct
     7261 caacagcacc tgcttcaact taccgtatgg ggcatcaaac aattgcaggc tcgggtcttg
     7321 gctgtcgaga ggtatttgcg ggatcagcaa cttttgggta tctggggttg ttccgggaag
     7381 ctcatctgca caacggctgt cccatggaat gccagttggt ccaacaagac cctggacatg
     7441 atctggaata acatgacttg gatggagtgg gagagagaaa tcgacaacta cacgggactc
     7501 atctatacct tgattgagga gtctcagaac caacaagaga agaacgagca ggaactgctg
     7561 gagttggaca agtgggccag tctgtggaat tggttcgata taaccaactg gctttggtac
     7621 atcaaaatat ttataatgat cgttggtggt ctgattggtc ttaggattgt tttcaccgtc
     7681 ctgagcattg tcaaccgcgt gaggcagggt tattctcccc tttcctttca aactcacttg
     7741 cccgccccta gggggccaga ccgccctgag gggattgaag aggagggcgg cgaccgcgac
     7801 cgagatagga gtgtgcggct cgtggatggg tttctggccc tcttctggga tgaccttcgg
     7861 tccctctgtc ttttctccta tcatcgcctg agggatcttc ttttgattgt agctaggatc
     7921 gtcgagctgt tggggcgccg gggatgggag gcccttaaat actggtggaa tttgttgcaa
     7981 tattggtccc aagagctcag gaacagtgcg gtgtctcttt tgaacgcgac agccatcgca
     8041 gtagccgagg gaaccgaccg agttatagag atagtccaac ggatctaccg agctatcctc
     8101 cacatcccaa cgagaattcg acaaggcctt gagcggctcc ttctttagta actcgaggcc
     8161 ggcaaggccg gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag
     8221 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
     8281 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
     8341 tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc
     8401 tgattatgat ccggctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     8461 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
     8521 cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaggtc gactctagag
     8581 gatcgatccc cgccccggac gaactaaacc tgactacgac atctctgccc cttcttcgcg
     8641 gggcagtgca tgtaatccct tcagttggtt ggtacaactt gccaactggg ccctgttcca
     8701 catgtgacac ggggggggac caaacacaaa ggggttctct gactgtagtt gacatcctta
     8761 taaatggatg tgcacatttg ccaacactga gtggctttca tcctggagca gactttgcag
     8821 tctgtggact gcaacacaac attgccttta tgtgtaactc ttggctgaag ctcttacacc
     8881 aatgctgggg gacatgtacc tcccaggggc ccaggaagac tacgggaggc tacaccaacg
     8941 tcaatcagag gggcctgtgt agctaccgat aagcggaccc tcaagagggc attagcaata
     9001 gtgtttataa ggcccccttg ttaaccctaa acgggtagca tatgcttccc gggtagtagt
     9061 atatactatc cagactaacc ctaattcaat agcatatgtt acccaacggg aagcatatgc
     9121 tatcgaatta gggttagtaa aagggtccta aggaacagcg atatctccca ccccatgagc
     9181 tgtcacggtt ttatttacat ggggtcagga ttccacgagg gtagtgaacc attttagtca
     9241 caagggcagt ggctgaagat caaggagcgg gcagtgaact ctcctgaatc ttcgcctgct
     9301 tcttcattct ccttcgttta gctaatagaa taactgctga gttgtgaaca gtaaggtgta
     9361 tgtgaggtgc tcgaaaacaa ggtttcaggt gacgccccca gaataaaatt tggacggggg
     9421 gttcagtggt ggcattgtgc tatgacacca atataaccct cacaaacccc ttgggcaata
     9481 aatactagtg taggaatgaa acattctgaa tatctttaac aatagaaatc catggggtgg
     9541 ggacaagccg taaagactgg atgtccatct cacacgaatt tatggctatg ggcaacacat
     9601 aatcctagtg caatatgata ctggggttat taagatgtgt cccaggcagg gaccaagaca
     9661 ggtgaaccat gttgttacac tctatttgta acaaggggaa agagagtgga cgccgacagc
     9721 agcggactcc actggttgtc tctaacaccc ccgaaaatta aacggggctc cacgccaatg
     9781 gggcccataa acaaagacaa gtggccactc ttttttttga aattgtggag tgggggcacg
     9841 cgtcagcccc cacacgccgc cctgcggttt tggactgtaa aataagggtg taataacttg
     9901 gctgattgta accccgctaa ccactgcggt caaaccactt gcccacaaaa ccactaatgg
     9961 caccccgggg aatacctgca taagtaggtg ggcgggccaa gataggggcg cgattgctgc
    10021 gatctggagg acaaattaca cacacttgcg cctgagcgcc aagcacaggg ttgttggtcc
    10081 tcatattcac gaggtcgctg agagcacggt gggctaatgt tgccatgggt agcatatact
    10141 acccaaatat ctggatagca tatgctatcc taatctatat ctgggtagca taggctatcc
    10201 taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc
    10261 taatttatat ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc
    10321 taatctatat ctgggtagta tatgctatcc taatctgtat ccgggtagca tatgctatcc
    10381 taatagagat tagggtagta tatgctatcc taatttatat ctgggtagca tatactaccc
    10441 aaatatctgg atagcatatg ctatcctaat ctatatctgg gtagcatatg ctatcctaat
    10501 ctatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat
    10561 ctatatctgg gtagtatatg ctatcctaat ttatatctgg gtagcatagg ctatcctaat
    10621 ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat
    10681 ctgtatccgg gtagcatatg ctatcctcat gcatatacag tcagcatatg atacccagta
    10741 gtagagtggg agtgctatcc tttgcatatg ccgccacctc ccaagggggc gtgaattttc
    10801 gctgcttgtc cttttcctgc tggttgctcc cattcttagg tgaatttaag gaggccaggc
    10861 taaagccgtc gcatgtctga ttgctcacca ggtaaatgtc gctaatgttt tccaacgcga
    10921 gaaggtgttg agcgcggagc tgagtgacgt gacaacatgg gtatgcccaa ttgccccatg
    10981 ttgggaggac gaaaatggtg acaagacaga tggccagaaa tacaccaaca gcacgcatga
    11041 tgtctactgg ggatttattc tttagtgcgg gggaatacac ggcttttaat acgattgagg
    11101 gcgtctccta acaagttaca tcactcctgc ccttcctcac cctcatctcc atcacctcct
    11161 tcatctccgt catctccgtc atcaccctcc gcggcagccc cttccaccat aggtggaaac
    11221 cagggaggca aatctactcc atcgtcaaag ctgcacacag tcaccctgat attgcaggta
    11281 ggagcgggct ttgtcataac aaggtcctta atcgcatcct tcaaaacctc agcaaatata
    11341 tgagtttgta aaaagaccat gaaataacag acaatggact cccttagcgg gccaggttgt
    11401 gggccgggtc caggggccat tccaaagggg agacgactca atggtgtaag acgacattgt
    11461 ggaatagcaa gggcagttcc tcgccttagg ttgtaaaggg aggtcttact acctccatat
    11521 acgaacacac cggcgaccca agttccttcg tcggtagtcc tttctacgtg actcctagcc
    11581 aggagagctc ttaaaccttc tgcaatgttc tcaaatttcg ggttggaacc tccttgacca
    11641 cgatgctttc caaaccaccc tccttttttg cgcctgcctc catcaccctg accccggggt
    11701 ccagtgcttg ggccttctcc tgggtcatct gcggggccct gctctatcgc tcccgggggc
    11761 acgtcaggct caccatctgg gccaccttct tggtggtatt caaaataatc ggcttcccct
    11821 acagggtgga aaaatggcct tctacctgga gggggcctgc gcggtggaga cccggatgat
    11881 gatgact
//
