LOCUS       sequence-240066-         685 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  CRISPR-mediated repression of human islet enhancer containing 
            T2D-associated variant rs7732130 (ZBED3 GWAS locus).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 685)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR
            rs773213...
FEATURES             Location/Qualifiers
     source          1..685
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     26..45
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        125..200
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 gnngttttaa attatntttt nnatggacta tcatatgctt accgtaactt gaaagtattt
       61 cgatttcttg gctttatata tcttgtggaa aggacgaaac accgatgcca cagacaagtt
      121 actcgtttta gagctagaaa tagcaagtta aaataaggct agtccgttat caacttgaaa
      181 aagtggcacc gagtcggtgc ttttttgaat tcgctagcta ggtcttgaaa ggggttgggg
      241 ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc tgggcgtggt
      301 tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc cgttcgcagc
      361 gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc tgctccgccc
      421 ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac ggaagccgca
      481 cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc gcgccgaccg
      541 cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag cggccggnaa
      601 ggggcggtgc gggaggcggg gtgtgnnnnn ntagngngnn nnnngnnnnn gcnnnnnnng
      661 ngnnnncnnt nnnnnnnnnt nnntt
//
