LOCUS       sequence-240319-        3819 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Lentiviral expression of PIK3CB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3819)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 3819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-PIK3CB
FEATURES             Location/Qualifiers
     source          1..3819
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             305..316
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(1187..1206)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1256..1272)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1256..1272)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1269..1291)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1280..1296
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1304..1310,1311..1328,1329..1334))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1349..1370
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1610..1627)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1781..2369)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1861..1880)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2540..3331,3332..3400))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3163..3182
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3401..3505)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3573..3591
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     LTR             join(3710..3819,1..124)
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
ORIGIN
        1 ggcttaagca gtgggttccc tagttagcca gagagctccc aggctcagat ctggtctaac
       61 cagagagacc cagtacaagc aaaaagcaga tcttgtcttc gttgggagtg aattagccct
      121 tccagtcccc ccttttcttt taaaaagtgg ctaagatcta cagctgcctt gtaagtcatt
      181 ggtcttaaag gatctcaggc ggggaggcgg cccaaaggga gatccgactc gtctgagggc
      241 gaaggcgaag acgcggaaga ggccgcagag ccggcagcag gccgcgggaa ggaaggtccg
      301 ctggattgag ggccgaaggg acgtagcaga aggacgtccc gcgcagaatc caggtggcaa
      361 cacaggcgag cagccaagga aaggacgatg atttccccga caacaccacg gaattgtcag
      421 tgcccaacag ccgagcccct gtccagcagc gggcaaggca agctttattg aggcttaagc
      481 agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac
      541 ccagtacagg caaaaagcag ctgcttatat gcaggatctg agggctcgcc actccccagt
      601 cccgcccagg ccacgcctcc ctggaaagtc cccagcggaa agtcccttgt agcaagctcg
      661 atatcagcag ttcttgaagt actccggatg cagctctcgg gccacgtgat gaaatgctag
      721 gcggctgtca aacctccact ctaacacttc tctctccggg tcatccatcc catgcaggct
      781 cacagggtgt aacaagctgg tgttctctcc tttattggcc tcttctacct tatctggctc
      841 aactggtact agcttgtagc accatccaaa ggtcagtgga tatctgaccc ctggccctgg
      901 tgtgtagttc tgctaatcag ggaagtagcc ttgtgtgtgg tagatccaca gatcaaggat
      961 atcttgtctt ctttgggagt gaattagccc ttccaactac taagtttgta gtacatattt
     1021 aacaaataca atttctttaa aatgaaaata attcagagga atcacaggtt tagagtaaat
     1081 gaaaccacag gtaattggca gtggtaatag ggtatggggt gggaagtttg ggatgatttt
     1141 ggttagcttg agttatccag ttgatccaga catgataaga tacattgatg agtttggaca
     1201 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaattcgta atcatgtcat
     1261 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     1321 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     1381 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca cggggagctt tttgcaaaag
     1441 cctaggcctc caaaaaagcc tcctcactac ttctggaata gctcagaggc cgaggcggcc
     1501 tcggcctctg cataaataaa aaaaattagt cagccatgag cttgctgcat taatgaatcg
     1561 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     1621 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     1681 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     1741 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     1801 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     1861 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     1921 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     1981 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     2041 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     2101 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     2161 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     2221 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     2281 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     2341 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     2401 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     2461 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     2521 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     2581 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     2641 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     2701 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     2761 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     2821 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     2881 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     2941 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     3001 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     3061 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     3121 gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg ggataatacc gcgccacata
     3181 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     3241 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     3301 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     3361 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     3421 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     3481 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
     3541 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
     3601 ttcaagaatg atctagccct ttccttaatt aacccgggcc tctcactctc tgatattcat
     3661 ttctttgcaa gttataaata ctgaataata agatgacatg aactactact gctagagatt
     3721 ttccacactg actaaaaggg tctgagggat ctctagttac cagagtcaca caacagacgg
     3781 gcacacacta cttgaagcac tcaaggcaag ctttattga
//
