LOCUS       sequence-240348-       10511 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expresses mouse Type I procollagen alpha-1 chain (Col1a1) with eGFP 
            replacing exon 2-3 retaining N-propeptide minor triple helix and 
            N-terminal cleavage site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10511)
  AUTHORS   Omari S, Makareeva E, Roberts-Pilgrim A, Mirigian L, Jarnik M, Ott 
            C, Lippincott-Schwartz J, Leikin S
  TITLE     Noncanonical autophagy at ER exit sites regulates procollagen 
            turnover.
  JOURNAL   Proc Natl Acad Sci U S A. 2018 Oct 23;115(43):E10099-E10108. doi: 
            10.1073/pnas.1814552115. Epub 2018 Oct 4.
  PUBMED    30287488
REFERENCE   2  (bases 1 to 10511)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1814552115"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2018-10-23- 23"; volume: "115"; issue: "43"; pages: 
            "E10099-E10108"
COMMENT     Sequence Label: eGFP-pro-alpha-1(I)
FEATURES             Location/Qualifiers
     source          1..10511
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(778..780,781..1488)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELY"
     primer_bind     complement(820..841)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1081..1100)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1428..1449
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5868..5885)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5874..6098
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6144..6572
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6231..6250)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6441..6462
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6581..6601)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6586..6915
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6766..6901
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6828..6847
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6982..7776
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7036..7055)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7646..7665
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7950..8071
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7987..8006)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8041..8060
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8120..8136)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8120..8136)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8133..8155)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8144..8160
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8168..8174,8175..8192,8193..8198))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8213..8234
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8351..8368)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8522..9110)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8602..8621)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9281..10072,10073..10141))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9904..9923
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10142..10246)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10321..10340)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagcgttt aaacttaagc ttatgtctag ggtctagaca tgttcagctt tgtggacctc
      721 cggctcctgc tcctcttagg ggccactgcc ctcctgacgc atggccaaga agatatcgtg
      781 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
      841 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
      901 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
      961 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
     1021 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     1081 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     1141 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     1201 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
     1261 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
     1321 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     1381 agcacccagt ccaagctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     1441 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacga gtcagaagat
     1501 gtaggagtcg agggacccaa gggagaccct ggcccccaag gcccaagggg acccgttggc
     1561 ccccctggac gagatggcat ccctggacag cctggacttc ctggtcctcc tggtccccct
     1621 gggccccccg gaccccctgg ccttggagga aactttgctt cccagatgtc ctatggctat
     1681 gatgaaaaat cagctggagt ttccgtgcct ggccccatgg gtccttctgg tcctcgtggt
     1741 ctccctggcc cccctggtgc acctggtcca caaggtttcc aaggcccccc tggtgaacct
     1801 ggcgagcctg gcggttcagg tccaatgggt ccccgaggtc cccctggccc tcctggcaag
     1861 aatggagatg atggggaagc tggcaagccc ggccgtcctg gtgagcgtgg acctcctgga
     1921 cctcagggtg ctcgtggatt gcctggaaca gctggcctcc ctggaatgaa gggacaccga
     1981 ggcttcagtg gtttggatgg tgccaaagga gatgctggtc ctgctggtcc taagggagag
     2041 cccggcagtc ctggtgaaaa cggagctcct ggccagatgg gtccccgagg tctgcccggt
     2101 gagagaggtc gccctggacc tcctggcact gctggtgctc gcggtaacga tggtgctgtt
     2161 ggtgctgctg gaccccctgg tcccaccggc cccactggcc ctcctggctt ccctggtgca
     2221 gttggtgcta agggtgaagc tggtccccaa ggagctagag gctctgaagg tccccagggt
     2281 gtgcgtggtg agcccggacc ccctggccct gctggtgctg ccggccctgc tggaaaccct
     2341 ggtgctgatg gacaacctgg cgctaaaggt gccaatggtg ctcctggtat tgctggtgct
     2401 cctggcttcc ctggtgcccg aggcccctct ggaccccagg gccccagcgg ccctccaggt
     2461 cccaagggta acagtggtga acctggtgct cctggcaaca aaggagacac tggtgccaaa
     2521 ggagaacccg gtgctactgg agttcaaggt cccccaggcc ctgccggaga agaaggaaaa
     2581 cgaggagccc gtggtgagcc tggaccttcc ggactgcctg gacctcctgg cgagcgtggt
     2641 ggacctggta gccgtggttt ccctggtgct gatggtgttg ctggccccaa gggtccttcc
     2701 ggtgaacgtg gtgctcccgg acctgctggt cccaaaggtt ctcctggtga agctggtcgc
     2761 cccggtgaag ctggtctccc tggtgccaag ggtctcactg gcagtcctgg cagccctggt
     2821 cctgatggca aaaccggccc ccctggtccc gctggtcaag atggtcgccc tggacccgca
     2881 ggtcctcctg gagcccgtgg ccaggctggt gtgatgggat tccctggacc taagggtacc
     2941 gctggagaac ctggaaaggc tggagagcga ggccttcccg gaccccctgg cgctgttggt
     3001 cctgctggca aagatggaga agctggagct cagggagccc ctggccctgc tggtcctgct
     3061 ggtgagagag gtgaacaagg tcccgctggc tcccctggat tccagggtct tcctggtcct
     3121 gccggtcctc ctggtgaagc aggcaagcct ggtgaacagg gtgttcctgg agaccttggt
     3181 gcccctggac cctctggcgc aagaggcgag agaggtttcc ctggtgaacg tggtgtacaa
     3241 ggtcccccag gtcctgctgg tccccgagga aacaatggtg cccccggcaa cgatggtgcc
     3301 aagggtgata ctggtgcccc cggagctccc ggtagccagg gtgcccccgg tcttcaggga
     3361 atgcctggtg aacgtggtgc agctggtctt ccaggtccta agggtgacag aggtgatgct
     3421 ggtcccaaag gtgctgatgg ttctcctggt aaagatggtg cccgtggtct gactggtccc
     3481 attggtcctc ctggccctgc tggtgcccct ggtgacaagg gtgaagctgg tcccagtggt
     3541 cctcccggtc ccaccggagc ccgtggtgct cccggagacc gtggtgaggc tggtccccct
     3601 ggtcctgctg gctttgccgg cccccctggt gctgatggcc aacctggtgc gaaaggtgaa
     3661 cctggtgata ctggtgttaa aggtgatgct ggtcctcctg gccctgctgg tcctgctgga
     3721 ccccccggcc ccattggtaa cgttggtgct cctggaccca aaggtcctcg tggtgctgct
     3781 ggtccccctg gtgctactgg cttccctggt gctgctggcc gtgtcggtcc ccctggtccc
     3841 tctggaaatg ctggaccccc tggccctccc ggtcccgttg gcaaagaagg gggcaaaggt
     3901 ccccgtggtg agactggccc tgctggacgt cctggtgaag ttggtccccc aggtcccccc
     3961 ggtcctgctg gtgagaaagg atctcctggt gctgatggac ctgctggctc tcctggtacc
     4021 cctggacctc agggtattgc tggacaacgt ggtgtggtcg gtcttcccgg tcagagagga
     4081 gaaagaggct tccctggtct tcctggcccc tctggtgaac ctggcaaaca aggtccttct
     4141 ggatcaagtg gtgaacgcgg tccccctggc cccatggggc cccctggatt ggctggtccc
     4201 cctggtgaat ctggacgtga gggatcccct ggtgctgaag gctcccctgg aagggatggt
     4261 gctcccgggg ccaagggtga ccgtggtgag actggccctg ctggcccccc tggtgcccct
     4321 ggtgctcccg gtgctcccgg ccctgttggt cccgctggca agaatggcga tcgtggtgag
     4381 actggtcctg ctggtcctgc tggtcccatt ggccctgctg gtgcccgtgg ccctgctgga
     4441 ccccaaggcc cccgtggtga caagggtgag acaggcgaac aaggtgacag aggcataaag
     4501 ggtcatcgtg gcttctctgg tctccagggt cctcctggtt ctcctggttc tcctggtgaa
     4561 caaggcccct ctggagcttc aggtcctgca ggcccccggg gtccccctgg ctctgctggt
     4621 tctcctggca aagacggact caacggtctc cctggcccca ttggtccccc tggtcctcga
     4681 ggtcgcactg gtgacagcgg ccctgctggt ccccccggcc ctcctggacc ccctggccct
     4741 cctggacctc ccagtggcgg ttatgacttc agcttcctgc ctcagccacc tcaagagaag
     4801 tctcaagatg gtggccgcta ctaccgggcc gatgatgcta acgtggttcg tgaccgtgac
     4861 cttgaggtgg acaccaccct caagagcctg agtcagcaga ttgagaacat ccgcagcccc
     4921 gaaggcagcc gcaagaaccc tgcccgcaca tgccgcgacc tcaagatgtg ccactctgac
     4981 tggaagagcg gagagtactg gatcgaccct aaccaaggct gcaacctgga cgccatcaag
     5041 gtctactgca acatggagac aggtcagacc tgtgtgttcc ctactcagcc gtctgtgcct
     5101 cagaagaact ggtacatcag cccgaacccc aaggaaaaga agcacgtctg gtttggagag
     5161 agcatgaccg atggattccc gttcgagtac ggaagcgagg gctccgaccc cgccgatgtc
     5221 gctatccagc tgaccttcct gcgcctaatg tccaccgagg cctcccagaa catcacctat
     5281 cactgcaaga acagcgtagc ctacatggac cagcagactg gcaacctcaa gaaggccctg
     5341 ctcctccagg gatccaacga gatcgagctc agaggcgaag gcaacagtcg cttcacctac
     5401 agcacccttg tggacggctg cacgagtcac accggaactt ggggcaagac agtcatcgaa
     5461 tacaaaacca ccaagacctc ccgcctgccc atcatcgatg tggctccctt ggacattggt
     5521 gccccagacc aggaattcgg actagacatt ggccctgcct gcttcgtgta aactccctcc
     5581 accccaatct ggttccctcc cacccagccc acttttcccc aaccctggaa acagacgaac
     5641 aacccaaact caatttcccc caaaagccaa aaatatggga gataatttca catggacttt
     5701 ggaaaacatt ttttttcctt tgcattcacc tttcaaactt agtttttacc tttgaccaac
     5761 tgaacgtgac caaaaaccaa aagtgcattc aaccttacca aaaaagaaaa aaaaaaaaaa
     5821 agggcggccg ctcgagtcta gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc
     5881 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     5941 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     6001 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     6061 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
     6121 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     6181 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     6241 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     6301 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     6361 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     6421 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     6481 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     6541 tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg
     6601 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     6661 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     6721 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     6781 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     6841 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     6901 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag
     6961 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc
     7021 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
     7081 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
     7141 tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg
     7201 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
     7261 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
     7321 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
     7381 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
     7441 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
     7501 ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
     7561 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
     7621 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
     7681 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
     7741 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga
     7801 ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg
     7861 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
     7921 atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca
     7981 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     8041 gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct
     8101 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     8161 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     8221 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     8281 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     8341 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     8401 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     8461 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     8521 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     8581 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     8641 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     8701 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     8761 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     8821 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     8881 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     8941 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     9001 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     9061 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     9121 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     9181 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     9241 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     9301 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     9361 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     9421 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     9481 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     9541 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     9601 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     9661 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     9721 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     9781 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     9841 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     9901 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     9961 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
    10021 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
    10081 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
    10141 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
    10201 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
    10261 aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag
    10321 tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga
    10381 ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa
    10441 ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag
    10501 atatacgcgt t
//
