LOCUS       sequence-240925-        3444 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Gateway entry clone of Drosophila EBP50 cDNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3444)
  TITLE     Vilmos Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3444)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: EBP50-pDONR221
FEATURES             Location/Qualifiers
     source          1..3444
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     19..41
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     33..50
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     34..50
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    66..165
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1066..1165)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1182..1201)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1183..1201)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1206..1222)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1206..1222)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1324..1343)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1335..2144
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1402..1421)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2291..2879
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2780..2799
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3033..3050
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3209..3236)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3328..3414)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 cgctagcatg gatgttttcc cagtcacgac gttgtaaaac gacggccagt cttaagctcg
       61 ggccccaaat aatgatttta ttttgactga tagtgacctg ttcgttgcaa caaattgatg
      121 agcaatgctt ttttataatg ccaactttgt acaaaaaagc aggcttcacc atgtccacgc
      181 ccacttcccc gaagacgccc acaccgccca ctttgccacc gggcgtgacc aaaacatgtc
      241 acattgtgaa aaggcccgat ttcgatggct atggtttcaa tttgcattcg gagaaggtga
      301 aaccaggaca gtttattggc aaagtagatg cggattctcc ggcagaggca gccggcctga
      361 aggagggcga tcgcatcctg gaggtcaacg gggtgtccat tggcagcgag acccacaagc
      421 aggtggttgc caggatcaag gccattgcga atgaagtccg cttgctgctc atcgatgtgg
      481 atggcaaggc cttggaggtg aaaccggcat ctccgccagc cgctgcgtgc aatggaaacg
      541 gtagtgccag tcagaatgga tacgagggca ccaaacagga gatgcccgga gcaagtgcca
      601 atatcagtag catcagtatg gtgagcacca agcgatcctc aaatgccagc agcattcaga
      661 gcggcagtac catgaatgcc tccgatttgg atgtggtcga taggggaata ccggcagtcg
      721 ctgctccggt ggctatcacc ccgcctcccg ttcaaaatgg aagtaaaccc tcatcgccga
      781 ttaataataa cactttgatg agcacaccgc caccgccgtc cgctactaag gctggcatca
      841 acaacaatgg cagtgtttat aacaccaatg gaaatggtac aaatggcatg accacaccca
      901 ctacaccacc cccaccgacc agtggctata aggcgggcac cttgcattta ccaatgacgg
      961 ccgccgaaat gcgcgccaaa ttggcatcca agaagaagta cgatcccaag aacgagagtg
     1021 tggacctcaa gaagaagttc gacatcattc agaagctcgc aagcgaccca gctttcttgt
     1081 acaaagttgg cattataaga aagcattgct tatcaatttg ttgcaacgaa caggtcacta
     1141 tcagtcaaaa taaaatcatt atttgccatc cagctgatat cccctatagt gagtcgtatt
     1201 acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca
     1261 ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac ataaacagta
     1321 atacaagggg tgttatgagc catattcaac gggaaacgtc gaggccgcga ttaaattcca
     1381 acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg
     1441 cgacaatcta tcgcttgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca
     1501 aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat
     1561 ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca
     1621 ccactgcgat ccccggaaaa acagcattcc aggtattaga agaatatcct gattcaggtg
     1681 aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta
     1741 attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata
     1801 acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag
     1861 tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc actcatggtg
     1921 atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg
     1981 gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg
     2041 agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata
     2101 tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagaa ttggttaatt
     2161 ggttgtaaca ctggcagagc attacgctga cttgacggga cggcgcaagc tcatgaccaa
     2221 aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca
     2281 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     2341 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     2401 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     2461 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     2521 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     2581 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     2641 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     2701 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     2761 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     2821 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     2881 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     2941 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     3001 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     3061 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     3121 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg
     3181 ctagccagga agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt
     3241 agtttgatgc ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt
     3301 cacaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt tcaccgacaa
     3361 acaacagata aaacgaaagg cccagtcttc cgactgagcc tttcgtttta tttgatgcct
     3421 ggcagttccc tactctcgcg ttaa
//
