LOCUS       sequence-240950-        7718 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Targeting expression to neuronal and glial cell types in mice, 
            non-human primates, and human retina.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7718)
  AUTHORS   Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz 
            P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, 
            Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, 
            Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang 
            XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B
  TITLE     Targeting neuronal and glial cell types with synthetic promoter AAVs
            in mice, non-human primates and humans.
  JOURNAL   Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2.
  PUBMED    31285614
REFERENCE   2  (bases 1 to 7718)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2."
COMMENT     Sequence Label: 6_pAAV-ProA37-CatCh-GFP-WPRE
FEATURES             Location/Qualifiers
     source          1..7718
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   12..141
                     /label=AAV2 ITR
     regulatory      2162..2171
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2168..3094
                     /codon_start=1
                     /product="N-terminal fragment of channelrhodopsin-2 from 
                     the alga Chlamydomonas reinhardtii (Nagel et al., 2003)"
                     /label=ChR2
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGAV"
     CDS             join(3098..3100,3101..3103,3104..3817)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3098..3100,3101..3103,3104..3814)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3143..3164)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3404..3423)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3751..3772
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3845..4433
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3898..3918)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4316..4327)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4465..4941
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4664..4683)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   4981..5121
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4981..5110
                     /label=AAV2 ITR
     rep_origin      5196..5651
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5283..5302)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5493..5514
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5668..5687)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5787..5809
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5847..5865)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5933..6037
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6038..6106,6107..6898)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6256..6275)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7069..7657
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7558..7577
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgagcc gagttagctc tcttacagct
      181 gtgtggttcc attgtcatta ataagtgcga gtctctccta cttcctctgt gacttttgct
      241 tcttactcat tttacaagat caccacgtgt cttccctggg ttctaagcct taattggtca
      301 ctggtgtgta cagggcactt ctcaacctgt gggtcacgat ccttccacag gggccacata
      361 tcagatatcc tgcctatcag gtatttacga tacgattcct aacagtagca ctattacagg
      421 tatgaagtag cagtgaaaag aattttatgg ttgggggtcg ccaccacatg aggagccgta
      481 ttaggaaggt tgagaacggc tggtctaagg catatagctg atgatgtcaa aagacatatc
      541 atccatgggg gacagctgtt gctccttccg tcttctcctt cgctgggtcc tgggcccttg
      601 gcttccagca ggctgtgagg taaatgtggg atatgaagct gtatcctgag tcaattttac
      661 ctgacaagcc aagggaagga agcagatggt caggaagggg ccagccctca ggagacttcc
      721 ttcctcccca gctaggctac acctcatcat ttacttcagc ctccccagat agcgccacca
      781 gccgggggga catgcaagct agagggtgac atttcatgcc caaacccagg gaagtgagtc
      841 gcccagctag ctgaggccag tgtgtgctag cgttctgctt gcttctccca ctctccacac
      901 agtggcctct ctacagggca cctctacagg gcgtccaact cagccggcag cacattgcct
      961 ggcctcggct ggcctccatg atacctcatt cttgcatttc cacgtgaaca tcgagttttg
     1021 gggactgccc cctctgcttt aggatgctgc ccatcagcag aactattctg gtcaaagtgt
     1081 gatcctaccc atcagaccct cccgtgtttc aggcggtctg aggctttgtc agaactctgt
     1141 ggaatctccc tctgggaggc aggctctccc tccgattgat tgatggctcc cagccatctc
     1201 cttctctcac cttgagactt gggatggggt gaagtcgggg gagtcccagt ttcttggtcc
     1261 actgtttcaa gagtctgttg gctagaggga agggcacaag cttctcttca gagaaatctt
     1321 tattcctgca gccaggttca tccccgcact gccccttggc tgtaaaatcc ccagcccttg
     1381 tttcagtaca aagtccagtc tctctgcctt aacttagtag agaaaatgct ctggttagaa
     1441 cagtcttgaa taaatatgac attttagcag cttgacacac caaacaggtg cctaaggcgc
     1501 ccaaggtacc ttgatggtct tgtctttgtt gttcaagatg cggtagcctt cggaagagat
     1561 acctggcagt gcaagtccac attaggacgc agggaaaaga ggggcgtgtg ggtggggggt
     1621 gcggggggcg ggtgtctacg aatagctgca gcccaggatt ggccctgtag atcctcaagg
     1681 ctggtcagat cttttccctt catatcccag ctggttggaa gcaccctggc tgctgccaaa
     1741 ctgctcccca cagaccccag aggggcggct gcccgcagcc tggtttctaa tttcaaaatc
     1801 acgccacgtt ctgcaaacgt tgtgtgtttt aggtcaaata aacaactctg caaacatgta
     1861 aataagctgc tctgcactga ttgatcctgg gagagggtca gcgagggagg gaaggggagg
     1921 gagggaggga gggaccgaag ggggggaagg cagcggaaag taggggcagg cggtgagatt
     1981 ccacagctga gccaccacct cccgcccagg ccgaatgaag atgcacaatt tggattggag
     2041 gggaagggtc aggagctgct gacctttggg ctcaggccca ggccgctggc cacagtagct
     2101 cttggtccag tcggcgccgg aggtgtatca agcgctcatc ccgccctctc cagtcggatc
     2161 cgccaccatg gattatggag gcgccctgag tgccgttggg cgcgagctgc tatttgtaac
     2221 gaacccagta gtcgtcaatg gctctgtact tgtgcctgag gaccagtgtt actgcgcggg
     2281 ctggattgag tcgcgtggca caaacggtgc ccaaacggcg tcgaacgtgc tgcaatggct
     2341 tgctgctggc ttctccatcc tactgcttat gttttacgcc taccaaacat ggaagtcaac
     2401 ctgcggctgg gaggagatct atgtgtgcgc tatcgagatg gtcaaggtga ttctcgagtt
     2461 cttcttcgag tttaagaacc cgtccatgct gtatctagcc acaggccacc gcgtccagtg
     2521 gttgcgttac gccgagtggc ttctcacctg cccggtcatt tgcattcacc tgtcaaacct
     2581 gacgggcttg tccaacgact acagcaggcg caccatgggt ctgcttgtgt ctgatattgg
     2641 cacaattgtg tggggcgcca cttccgccat ggccaccgga tacgtcaagg tcatcttctt
     2701 ctgcctgggt ctgtgttatg gtgctaacac gttctttcac gctgccaagg cctacatcga
     2761 gggttaccac accgtgccga agggccggtg tcgccaggtg gtgactggca tggcttggct
     2821 cttcttcgta tcatggggta tgttccccat cctgttcatc ctcggccccg agggcttcgg
     2881 cgtcctgagc gtgtacggct ccaccgtcgg ccacaccatc attgacctga tgtcgaagaa
     2941 ctgctggggt ctgctcggcc actacctgcg cgtgctgatc cacgagcata tcctcatcca
     3001 cggcgacatt cgcaagacca ccaaattgaa cattggtggc actgagattg aggtcgagac
     3061 gctggtggag gacgaggccg aggctggcgc ggtacccatg gtgagcaagg gcgaggagct
     3121 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     3181 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     3241 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
     3301 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     3361 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     3421 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     3481 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     3541 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     3601 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     3661 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
     3721 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     3781 cgggatcact ctcggcatgg acgagctgta caagtgataa gaattcgata tcaagcttat
     3841 cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     3901 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     3961 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     4021 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     4081 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     4141 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     4201 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
     4261 gctcgcctat gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     4321 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     4381 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata
     4441 ccgagcgctg ctcgagagat ctacgggtgg catccctgtg acccctcccc agtgcctctc
     4501 ctggccctgg aagttgccac tccagtgccc accagccttg tcctaataaa attaagttgc
     4561 atcattttgt ctgactaggt gtccttctat aatattatgg ggtggagggg ggtggtatgg
     4621 agcaaggggc aagttgggaa gacaacctgt agggcctgcg gggtctattg ggaaccaagc
     4681 tggagtgcag tggcacaatc ttggctcact gcaatctccg cctcctgggt tcaagcgatt
     4741 ctcctgcctc agcctcccga gttgttggga ttccaggcat gcatgaccag gctcagctaa
     4801 tttttgtttt tttggtagag acggggtttc accatattgg ccaggctggt ctccaactcc
     4861 taatctcagg tgatctaccc accttggcct cccaaattgc tgggattaca ggcgtgaacc
     4921 actgctccct tccctgtcct tctgattttg taggtaacca cgtgcggacc gagcggccgc
     4981 aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg
     5041 ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc
     5101 gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt tctccttacg catctgtgcg
     5161 gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag
     5221 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     5281 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     5341 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     5401 aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     5461 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     5521 actcaaccct atctcgggct attcttttga tttataaggg attttgccga tttcggccta
     5581 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     5641 gtttacaatt ttatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     5701 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     5761 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     5821 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     5881 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     5941 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     6001 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     6061 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     6121 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     6181 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     6241 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     6301 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     6361 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     6421 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     6481 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     6541 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     6601 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     6661 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     6721 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     6781 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     6841 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     6901 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     6961 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     7021 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     7081 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     7141 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     7201 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     7261 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     7321 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     7381 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     7441 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     7501 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     7561 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     7621 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     7681 acggttcctg gccttttgct ggccttttgc tcacatgt
//
