LOCUS       sequence-241024-        2453 bp    DNA     circular SYN 14-APR-2025
DEFINITION  GBPart - Minimal Promoter for expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Sarrion-Perdigones A, Chang L, Gonzalez Y, Gallego-Flores T, Young 
            DW, Venken KJT
  TITLE     Examining multiple cellular pathways at once using multiplex 
            hextuple luciferase assaying.
  JOURNAL   Nat Commun. 2019 Dec 13;10(1):5710. doi: 10.1038/s41467-019-13651-y.
  PUBMED    31836712
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-12-13"; volume: "10(1):5710. doi"; pages: " 
            10.1038/s41467-019-13651-y"
COMMENT     Sequence Label: pMiniP
FEATURES             Location/Qualifiers
     source          1..2453
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     66..87
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     243..260
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     244..260
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     267..286
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        267..285
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(365..396)
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     promoter        complement(479..497)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(480..497)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(513..529)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(513..529)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(644..1232)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(724..743)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        1347..1449
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1450..2109
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1516..1535)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      join(2222..2453,1..224)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2309..2328)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tagtgggcca gcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
       61 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
      121 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
      181 aaaatttaac gcgaatttta acaaaatatt aacgcttaca attttaacat tgtagaaacg
      241 catgtaaaac gacggccagt gaattgtaat acgactcact atagggtgaa ttccgcttag
      301 gaccttagat attgatctca aggtctcgca ttggtggctt taccaacagt accggattgc
      361 caagctggaa gtcgagcttc cattatatac cctctagtgt ctaagcttgg ccgccgaggc
      421 cagatttgat atcctcgagg gacgagacca ctcatcgccc aatcactagt gaattcgttc
      481 tatagtgtca cctaaatacc atagagccgt tggtcatagc tgtttcctgt ccaggcatca
      541 tgacacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
      601 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
      661 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
      721 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
      781 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
      841 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
      901 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
      961 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     1021 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     1081 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     1141 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     1201 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     1261 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     1321 ggatcttcac ctagatcctt ttgtgatgat cggcacgtaa gaggttccaa ctttcaccat
     1381 aatgaaataa gatcactacc gggcgtattt tttgagttat cgagattttc aggagctaag
     1441 gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat
     1501 cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt
     1561 cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg
     1621 gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaattccg tatggcaatg
     1681 aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag
     1741 caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta
     1801 cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg
     1861 tttattgaga atatgttttt cgtgtcagcc aatccctggg tgagtttcac cagttttgat
     1921 ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat
     1981 acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat
     2041 ggcttccatg tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc
     2101 ggggcgtaat ttttttaagg cagttattgg tgcccttaaa cgcctggttg ctacgcctga
     2161 ataagtgata ataagcggat gaatggcaga aattcgatga taagctgtca aacatgatgt
     2221 tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     2281 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     2341 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     2401 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acg
//
