LOCUS       sequence-241702-         969 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Plant transformation vector for stable expression of mitochondria 
            targeted GFP1-10 in plant cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Sharma M, Kretschmer C, Lampe C, Stuttmann J, Klosgen RB
  TITLE     Targeting specificity of nuclear-encoded organelle proteins with a 
            self-assembling split-fluorescent protein toolkit.
  JOURNAL   J Cell Sci. 2019 Jun 3;132(11). pii: jcs.230839. doi: 
            10.1242/jcs.230839.
  PUBMED    31085714
REFERENCE   2  (bases 1 to 969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2019-06-3"; volume: "132(11). pii: jcs.230839. doi"; 
            pages: " 10.1242/jcs.230839"
COMMENT     Sequence Label: pLSU4mg10
FEATURES             Location/Qualifiers
     source          1..969
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             304..945
                     /codon_start=1
                     /product="fragment of superfolder GFP consisting of the 
                     first 10 beta-strands"
                     /label=GFP(1-10)
                     /note="associates with GFP11, the 11th beta-strand, to 
                     reconstitute fluorescent GFP (Cabantous et al., 2005; 
                     Kamiyama et al., 2016)"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATIGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     KYKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQTVLSKDPNEK"
ORIGIN
        1 atgcttcgag tagcaggtag aaggctttct tcttcagccg ctagatcttc atctaccttc
       61 tttacaagaa gctctttcac cgttaccgat gattcgtctc cggcaagatc tccttctccg
      121 tcactcacct cttcgtttct cgatcaaatc agaggtttct catctaattc ggtttctccc
      181 gcacatcagt tgggtttagt ctcagatctt ccagccacag tggctgctat taagaatccc
      241 agttcaaaaa ttgtatatga tgactccaac catgagcgtt atccacctgg agatcctagc
      301 gcaatgtcca aaggagaaga actgtttacc ggtgttgtgc caattttggt tgaactcgat
      361 ggtgatgtca acggacataa gttctcagtg agaggcgaag gagaaggtga cgccaccatt
      421 ggaaaattga ctcttaaatt catctgtact actggtaaac ttcctgtacc atggccgact
      481 ctcgtaacaa cgcttacgta cggagttcag tgcttttcga gatacccaga ccatatgaaa
      541 agacatgact tttttaagtc ggctatgcct gaaggttacg tgcaagaaag aacaatttcg
      601 ttcaaagatg atggaaaata taaaactaga gcagttgtta aatttgaagg agatactttg
      661 gttaaccgca ttgaactgaa aggaacagat tttaaagaag atggtaatat tcttggacac
      721 aaactcgaat acaattttaa tagtcataac gtatacatca ctgctgataa gcaaaagaac
      781 ggaattaaag cgaatttcac agtacgccat aatgtagaag atggcagtgt tcaacttgcc
      841 gaccattacc aacaaaacac ccctattgga gacggtccgg tacttcttcc tgataatcac
      901 tacctctcaa cacaaacagt cctgagcaaa gatccaaatg aaaaaggaac aggtggcggc
      961 ggaagttag
//
