LOCUS       sequence-241706-       10171 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Lentiviral expression of SGK3__KRT9 fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10171)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 10171)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-SGK3__KRT9
FEATURES             Location/Qualifiers
     source          1..10171
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3970..3994)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5231..5251)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             5305..5346
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5367..5934
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5384..5936
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5528..5545)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5755..5774
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5948..6547
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5948..5967)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6444..6464
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6561..7149
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6614..6634)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7032..7043)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7224..7457
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7576..7594)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7662..7766
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7767..7835,7836..8627)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7985..8004)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8798..9386
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9287..9306
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9540..9557
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9797..9818
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9833..9838,9839..9856,9857..9863)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9871..9887
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9876..9898
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9895..9911
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9895..9911
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9961..9980
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagt tggcatgcaa agagatcaca ccatggacta caaggaaagc
     3481 tgcccaagtg taagcattcc cagctccgat gaacacagag agaaaaagaa gaggtttact
     3541 gtttataaag ttctggtttc agtgggaaga agtgaatggt ttgtcttcag gagatatgca
     3601 gagtttgata aactttataa cactttaaaa aaacagtttc ctgctatggc cctgaagatt
     3661 cctgccaaga gaatatttgg tgataatttt gatccagatt ttattaaaca aagacgagca
     3721 ggactaaacg aattcattca gaacctagtt aggtatccag aactttataa ccatccagat
     3781 gtcagagcat tccttcaaat ggacagtcca aaacaccagt cagatccatc tgaagatgag
     3841 gatgaaagaa gttctcagaa gctacactct acctcacaga acatcaacct gggaccgtct
     3901 ggaaatcctc atgccaaacc aactgacttt gatttcttaa aagttattgg aaaaggcagc
     3961 tttggcaagt cccagctttc ttgtacaaag tggttcattg tggacctgac agtgggcaac
     4021 aacaaaactc tcctggacat tgacaacact cgcatgacac tggatgactt caggataaag
     4081 tttgagatgg agcaaaacct gcggcaagga gtggatgctg acatcaatgg cctgcggcag
     4141 gtgctggaca atctgaccat ggagaagtct gacctggaga tgcagtatga gactctgcag
     4201 gaggagctga tggccctcaa gaagaatcat aaggaggaga tgagtcagct gactgggcag
     4261 aacagtggag atgtcaatgt ggagataaac gttgctcctg gcaaagatct caccaagacc
     4321 ctcaatgaca tgcgtcagga gtatgagcag ctcattgcta agaacagaaa ggacatcgag
     4381 aatcaatatg agactcagat aacccagatc gagcatgagg tatccagtag tggtcaggag
     4441 gtgcagtcca gtgccaagga ggtgacccag ctccggcacg gtgtccagga gttggagatt
     4501 gagctgcagt ctcagctcag caagaaagca gctctggaga agagcttgga agacacgaag
     4561 aaccgctact gtggccagct gcagatgatc caggagcaga tcagtaactt ggaggcccag
     4621 atcactgacg tccggcaaga gatcgagtgc cagaatcagg aatacagcct tctgctcagc
     4681 attaagatgc ggctggagaa ggaaatcgag acctaccaca acctccttga gggaggccag
     4741 gaagactttg aatcctccgg agctggaaaa attggccttg gaggtcgagg aggaagtgga
     4801 ggcagttatg gaagaggatc caggggagga agtggaggca gctatggtgg aggaggaagt
     4861 ggaggtggct atggtggagg aagtgggtcc aggggaggaa gtggaggcag ctacggtgga
     4921 ggaagtggtt ctggaggagg tagtggaggt ggctatggtg gaggaagtgg aggtggccat
     4981 agcggaggaa gtggaggtgg tcatagtgga ggaagtgggg gcaactatgg aggaggaagt
     5041 ggctctggag gaggaagtgg gggtggctat ggtggaggaa gtgggtccag gggaggaagt
     5101 ggaggcagcc atggtggagg aagtggtttt ggaggtgaaa gtggaggcag ctacggaggc
     5161 ggtgaagaag cgagtggaag tggtggcggc tacggaggag gaagcggaaa atcatcccat
     5221 tcctagcacc caacttttct atacaaagtg gttgatatcc agcacagtgg cggccgctcg
     5281 agtctagagg gcccgcggtt cgaaggtaag cctatcccta accctctcct cggtctcgat
     5341 tctacgcgta ccggttagta atgagatccc tccccccccc ctaacgttac tggccgaagc
     5401 cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct
     5461 tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt
     5521 ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct
     5581 ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc
     5641 ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag
     5701 gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc
     5761 tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga
     5821 tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt
     5881 ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataatatggc
     5941 cacacatatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag
     6001 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
     6061 tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
     6121 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac
     6181 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
     6241 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
     6301 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg
     6361 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
     6421 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
     6481 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
     6541 tgcctaaatc gatagatcct aatcaacctc tggattacaa aatttgtgaa agattgactg
     6601 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     6661 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     6721 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     6781 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     6841 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     6901 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     6961 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     7021 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     7081 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     7141 cctccccgcc tgagatcctt taagaccaat gacttacaag gcagctgtag atcttagcca
     7201 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct
     7261 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     7321 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     7381 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     7441 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc
     7501 aaagaaatga atatcagaga gtgagaggcc cgggttaatt aaggaaaggg ctagatcatt
     7561 cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     7621 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     7681 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     7741 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     7801 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     7861 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     7921 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     7981 ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc
     8041 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     8101 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     8161 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     8221 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     8281 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     8341 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     8401 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     8461 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     8521 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     8581 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     8641 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     8701 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     8761 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     8821 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     8881 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     8941 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     9001 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     9061 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     9121 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     9181 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     9241 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     9301 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     9361 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     9421 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     9481 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     9541 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     9601 gttggccgat tcattaatgc agcaagctca tggctgacta atttttttta tttatgcaga
     9661 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     9721 cctaggcttt tgcaaaaagc tccccgtggc acgacaggtt tcccgactgg aaagcgggca
     9781 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     9841 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     9901 acagctatga catgattacg aatttcacaa ataaagcatt tttttcactg cattctagtt
     9961 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag
    10021 ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctgt
    10081 ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta
    10141 tttgttaaat atgtactaca aacttagtag t
//
