LOCUS       sequence-241736-       11860 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Lentiviral expression of KCNQ5__ALK fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11860)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 11860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-KCNQ5__ALK
FEATURES             Location/Qualifiers
     source          1..11860
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3843..3867)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6920..6940)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             6994..7035
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    7056..7623
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    7073..7625
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7217..7234)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7444..7463
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7637..8236
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7637..7656)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8133..8153
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8250..8838
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8303..8323)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8721..8732)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8913..9146
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9265..9283)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9351..9455
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9456..9524,9525..10316)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9674..9693)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10487..11075
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10976..10995
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11229..11246
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11486..11507
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11522..11527,11528..11545,11546..11552)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11560..11576
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11565..11587
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11584..11600
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11584..11600
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11650..11669
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagt tggcatgccc cgccaccacg cgggaggaga ggagggcggc
     3481 gccgccgggc tctgggtgaa gagcggcgca gcggcggcgg cggcgggcgg ggggcgcttg
     3541 ggcagcggca tgaaggatgt ggagtcgggc cggggcaggg tgctgctgaa ctcggcagcc
     3601 gccaggggcg acggcctgct actgctgggc acccgcgcgg ccacgcttgg tggcggcggc
     3661 ggtggcctga gggagagccg ccggggcaag cagggggccc ggatgagcct gctggggaag
     3721 ccgctctctt acacgagtag ccagagctgc cggcgcaacg tcaagtaccg gcgggtgcag
     3781 aactacctgt acaacgtgct ggagagaccc cgcggctggg cgttcatcta ccacgctttc
     3841 gttcccagct ttcttgtaca aagtggttcg ttctggctgc agatggtcgc atggtgggga
     3901 caaggatcca gagccatcgt ggcttttgac aatatctcca tcagcctgga ctgctacctc
     3961 accattagcg gagaggacaa gatcctgcag aatacagcac ccaaatcaag aaacctgttt
     4021 gagagaaacc caaacaagga gctgaaaccc ggggaaaatt caccaagaca gacccccatc
     4081 tttgacccta cagttcattg gctgttcacc acatgtgggg ccagcgggcc ccatggcccc
     4141 acccaggcac agtgcaacaa cgcctaccag aactccaacc tgagcgtgga ggtggggagc
     4201 gagggccccc tgaaaggcat ccagatctgg aaggtgccag ccaccgacac ctacagcatc
     4261 tcgggctacg gagctgctgg cgggaaaggc gggaagaaca ccatgatgcg gtcccacggc
     4321 gtgtctgtgc tgggcatctt caacctggag aaggatgaca tgctgtacat cctggttggg
     4381 cagcagggag aggacgcctg ccccagtaca aaccagttaa tccagaaagt ctgcattgga
     4441 gagaacaatg tgatagaaga agaaatccgt gtgaacagaa gcgtgcatga gtgggcagga
     4501 ggcggaggag gagggggtgg agccacctac gtatttaaga tgaaggatgg agtgccggtg
     4561 cccctgatca ttgcagccgg aggtggtggc agggcctacg gggccaagac agacacgttc
     4621 cacccagaga gactggagaa taactcctcg gttctagggc taaacggcaa ttccggagcc
     4681 gcaggtggtg gaggtggctg gaatgataac acttccttgc tctgggccgg aaaatctttg
     4741 caggagggtg ccaccggagg acattcctgc ccccaggcca tgaagaagtg ggggtgggag
     4801 acaagagggg gtttcggagg gggtggaggg gggtgctcct caggtggagg aggcggagga
     4861 tatataggcg gcaatgcagc ctcaaacaat gaccccgaaa tggatgggga agatggggtt
     4921 tccttcatca gtccactggg catcctgtac accccagctt taaaagtgat ggaaggccac
     4981 ggggaagtga atattaagca ttatctaaac tgcagtcact gtgaggtaga cgaatgtcac
     5041 atggaccctg aaagccacaa ggtcatctgc ttctgtgacc acgggacggt gctggctgag
     5101 gatggcgtct cctgcattgt gtcacccacc ccggagccac acctgccact ctcgctgatc
     5161 ctctctgtgg tgacctctgc cctcgtggcc gccctggtcc tggctttctc cggcatcatg
     5221 attgtgtacc gccggaagca ccaggagctg caagccatgc agatggagct gcagagccct
     5281 gagtacaagc tgagcaagct ccgcacctcg accatcatga ccgactacaa ccccaactac
     5341 tgctttgctg gcaagacctc ctccatcagt gacctgaagg aggtgccgcg gaaaaacatc
     5401 accctcattc ggggtctggg ccatggcgcc tttggggagg tgtatgaagg ccaggtgtcc
     5461 ggaatgccca acgacccaag ccccctgcaa gtggctgtga agacgctgcc tgaagtgtgc
     5521 tctgaacagg acgaactgga tttcctcatg gaagccctga tcatcagcaa attcaaccac
     5581 cagaacattg ttcgctgcat tggggtgagc ctgcaatccc tgccccggtt catcctgctg
     5641 gagctcatgg cggggggaga cctcaagtcc ttcctccgag agacccgccc tcgcccgagc
     5701 cagccctcct ccctggccat gctggacctt ctgcacgtgg ctcgggacat tgcctgtggc
     5761 tgtcagtatt tggaggaaaa ccacttcatc caccgagaca ttgctgccag aaactgcctc
     5821 ttgacctgtc caggccctgg aagagtggcc aagattggag acttcgggat ggcccgagac
     5881 atctacaggg cgagctacta tagaaaggga ggctgtgcca tgctgccagt taagtggatg
     5941 cccccagagg ccttcatgga aggaatattc acttctaaaa cagacacatg gtcctttgga
     6001 gtgctgctat gggaaatctt ttctcttgga tatatgccat accccagcaa aagcaaccag
     6061 gaagttctgg agtttgtcac cagtggaggc cggatggacc cacccaagaa ctgccctggg
     6121 cctgtatacc ggataatgac tcagtgctgg caacatcagc ctgaagacag gcccaacttt
     6181 gccatcattt tggagaggat tgaatactgc acccaggacc cggatgtaat caacaccgct
     6241 ttgccgatag aatatggtcc acttgtggaa gaggaagaga aagtgcctgt gaggcccaag
     6301 gaccctgagg gggttcctcc tctcctggtc tctcaacagg caaaacggga ggaggagcgc
     6361 agcccagctg ccccaccacc tctgcctacc acctcctctg gcaaggctgc aaagaaaccc
     6421 acagctgcag agatctctgt tcgagtccct agagggccgg ccgtggaagg gggacacgtg
     6481 aatatggcat tctctcagtc caaccctcct tcggagttgc acaaggtcca cggatccaga
     6541 aacaagccca ccagcttgtg gaacccaacg tacggctcct ggtttacaga gaaacccacc
     6601 aaaaagaata atcctatagc aaagaaggag ccacacgaca ggggtaacct ggggctggag
     6661 ggaagctgta ctgtcccacc taacgttgca actgggagac ttccgggggc ctcactgctc
     6721 ctagagccct cttcgctgac tgccaatatg aaggaggtac ctctgttcag gctacgtcac
     6781 ttcccttgtg ggaatgtcaa ttacggctac cagcaacagg gcttgccctt agaagccgct
     6841 actgcccctg gagctggtca ttacgaggat accattctga aaagcaagaa tagcatgaac
     6901 cagcctgggc cctgacaccc aacttttcta tacaaagtgg ttgatatcca gcacagtggc
     6961 ggccgctcga gtctagaggg cccgcggttc gaaggtaagc ctatccctaa ccctctcctc
     7021 ggtctcgatt ctacgcgtac cggttagtaa tgagatccct cccccccccc taacgttact
     7081 ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata
     7141 ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt
     7201 cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa
     7261 gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag
     7321 cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca
     7381 cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc
     7441 aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat
     7501 tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta
     7561 aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga
     7621 taatatggcc acacatatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
     7681 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
     7741 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac
     7801 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt
     7861 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat
     7921 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc
     7981 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca
     8041 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg
     8101 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg
     8161 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg
     8221 caagcccggt gcctaaatcg atagatccta atcaacctct ggattacaaa atttgtgaaa
     8281 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     8341 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     8401 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     8461 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     8521 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     8581 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     8641 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     8701 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     8761 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     8821 tttgggccgc ctccccgcct gagatccttt aagaccaatg acttacaagg cagctgtaga
     8881 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     8941 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     9001 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     9061 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     9121 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt
     9181 ataacttgca aagaaatgaa tatcagagag tgagaggccc gggttaatta aggaaagggc
     9241 tagatcattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     9301 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     9361 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     9421 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     9481 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     9541 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     9601 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     9661 cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac
     9721 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     9781 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     9841 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     9901 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     9961 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
    10021 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
    10081 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
    10141 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
    10201 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
    10261 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
    10321 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
    10381 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
    10441 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
    10501 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
    10561 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
    10621 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
    10681 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
    10741 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
    10801 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
    10861 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
    10921 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
    10981 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
    11041 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
    11101 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
    11161 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
    11221 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
    11281 tccccgcgcg ttggccgatt cattaatgca gcaagctcat ggctgactaa ttttttttat
    11341 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
    11401 ttttggaggc ctaggctttt gcaaaaagct ccccgtggca cgacaggttt cccgactgga
    11461 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
    11521 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
    11581 acacaggaaa cagctatgac atgattacga atttcacaaa taaagcattt ttttcactgc
    11641 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga
    11701 taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca
    11761 attacctgtg gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa
    11821 gaaattgtat ttgttaaata tgtactacaa acttagtagt
//
