LOCUS       sequence-241881-         930 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B 
            Flag-Cas7 driven by hUbC promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Pickar-Oliver A, Black JB, Lewis MM, Mutchnick KJ, Klann TS, 
            Gilcrest KA, Sitton MJ, Nelson CE, Barrera A, Bartelt LC, Reddy TE, 
            Beisel CL, Barrangou R, Gersbach CA
  TITLE     Targeted transcriptional modulation with type I CRISPR-Cas systems 
            in human cells.
  JOURNAL   Nat Biotechnol. 2019 Sep 23. pii: 10.1038/s41587-019-0235-7. doi: 
            10.1038/s41587-019-0235-7.
  PUBMED    31548729
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Sep 23. pii: 10.1038/s41587-019-0235-7. doi: 
            10.1038/s41587-019-0235-7."
COMMENT     Sequence Label: pLenti-LmoCas7
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..27
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             34..54
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 atggactaca aggacgatga tgacaagatg gcccccaaga agaaacggaa agtccgcggc
       61 atgaagaaca agggactggc aatgactatc attttccaag ccgaaagcgc caattacggc
      121 gaatccctgg ggaacatttc aagcctgaag aagatctcta ggaacaacgg agatcagtac
      181 acctacatct cccgccaagc gatccggtac aatcttatgg atcagatcgg agaaaaggaa
      241 gcccctgtga aggctgaagg cggcggagac aaaaaggtca tccagttcct gtccgaggcc
      301 acgatcaccg acttcccgga actcgacttt ttcggctacc tgaaaaccga aaagggttcc
      361 gggggacaga agcgcagcgc caaagtccga ctgtcgaacg ccatctcgct ggagactttt
      421 aagggcgacc tggatttcct cactaacaag ggacaggccg acaagctgaa cgaaaacatg
      481 aacattgcgc aggcagagat ccacaagtcc tactaccgct acaccatcac catcgacctc
      541 gaccagatcg gtatcgacgg ggagattgct ctggacaaca aggaaaaggc ccggagagtg
      601 aagaagctga tggacactgt ggcgttcctc tatcgggaca ttaggggcag acgggaggac
      661 ctcaagcccc tgttcgtgat cggtggagtg tatgacgtga agaacccggt gttccaaaac
      721 attgtggatg tggccgacaa caatattgtg atcaagaaca ttaaggattt gctgacctac
      781 gaggacatca aggagaacac cagagtcggc atcattgatg ggcagttcgc caactccgac
      841 gaagtgaaag tcgagctgaa ggccgagtcc gtgccagctt tcttcaacga catcaaggga
      901 aagattgatg cgtactacga gagcaactga
//
